LOCUS       sequence-44447-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS295
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..71)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(56..72)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(685..703)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gagcggccgc actagtggat cccccgggct gcaggaattg gatatcaagc ttatcgatac
       61 cgtcgacctc gacaatgcca gaacttggat ttagcatatc aggtggtgtc gggggtagag
      121 gaaacccatt cagacctgat gatgatggta tatttgtaac aagggtacaa cctgaaggac
      181 cagcatcaaa attactgcag ccaggtgata aaattattca ggctaatggc tacagtttta
      241 taaatattga acatggacaa gcagtgtcct tgctaaaaac tttccagaat acagttgaac
      301 tcatcattgt acgagaagtt ggtaacggtg caaaacaaga gattcgagtg agggttgaaa
      361 aagacggtgg ttctggaggc gtttcttctg ttccgaccaa tctggaagtt gttgctgcga
      421 ccccgactag cctgctgatc agctgggatg cgtactacga ctctcatgtt tcttattacc
      481 gtatcacgta cggtgaaacc ggtggtaact ccccggttca ggaattcact gtacctggtt
      541 ccaagtctac tgctaccatc agcggcctga aaccgggtgt tgactatacc atcactgtat
      601 acgcacatta caactaccat tactactcta gcccaatctc gattaactac cgtacctcta
      661 gactcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
      721 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
      781 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
      841 acgcgtgtac
//
