LOCUS       sequence-444525-       11515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-on lentiviral vector for controlled expression of GDNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Gutekunst CA, Tung JK, McDougal ME, Gross RE
  TITLE     C3 transferase gene therapy for continuous conditional RhoA 
            inhibition.
  JOURNAL   Neuroscience. 2016 Dec 17;339:308-318. doi: 
            10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13.
  PUBMED    27746349
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2016-12-17"; volume: "339:308-318. doi"; 
            pages: " 10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13"
COMMENT     Sequence Label: pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB
FEATURES             Location/Qualifiers
     source          1..11515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2029..2146
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2185..2684
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2220..2243)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2617..2636
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3382..3398
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3382..3398
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    3416..3983
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3434..3985
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3578..3595)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3805..3824
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3988..4608
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4061..4080)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4609..4629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4732..4926
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5065..5081)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5066..5082)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5096..5684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5149..5169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5567..5578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5822..5840,5841..5863,5864..5882,5883..5905,5906..5924,
                     5925..5947,5948..5966,5967..5989,5990..6008,6009..6031,
                     6032..6050,6051..6073,6074..6092)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             6131..6311
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6336..6354)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6337..6354)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6644..6663)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6877..6896)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6996..7018
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7056..7074)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7142..7246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7247..7315,7316..8107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7465..7484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8278..8866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8767..8786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9020..9037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9107..9127)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9112..9441
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9292..9427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10575..10640
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10770..10790
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11215..11349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11252..11271)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11306..11325
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     2041 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2101 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac
     2161 tagcctcgac gatggtcgag taccgggtag gggaggcgct tttcccaagg cagtctggag
     2221 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     2281 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     2341 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     2401 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     2461 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     2521 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     2581 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     2641 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctct agcgggatcc
     2701 cgtcgagaat tagcttcgcc accatgaagt tatgggatgt cgtggctgtc tgcctggtgc
     2761 tgctccacac cgcgtccgcc ttcccgctgc ccgccggtaa gaggcctccc gaggcgcccg
     2821 ccgaagaccg ctccctcggc cgccgccgcg cgcccttcgc gctgagcagt gactcaaata
     2881 tgccagagga ttatcctgat cagttcgatg atgtcatgga ttttattcaa gccaccatta
     2941 aaagactgaa aaggtcacca gataaacaaa tggcagtgct tcctagaaga gagcggaatc
     3001 ggcaggctgc agctgccaac ccagagaatt ccagaggaaa aggtcggaga ggccagaggg
     3061 gcaaaaaccg gggttgtgtc ttaactgcaa tacatttaaa tgtcactgac ttgggtctgg
     3121 gctatgaaac caaggaggaa ctgattttta ggtactgcag cggctcttgc gatgcagctg
     3181 agacaacgta cgacaaaata ttgaaaaact tatccagaaa tagaaggctg gtgagtgaca
     3241 aagtagggca ggcatgttgc agacccatcg cctttgatga tgacctgtcg tttttagatg
     3301 ataacctggt ttaccatatt ctaagaaagc attccgctaa aaggtgtgga tgtatctgag
     3361 gatcaattca cgcgtggtac ctctagaact agtggatccc ccgggctgca ggaattccgc
     3421 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     3481 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     3541 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     3601 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     3661 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     3721 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     3781 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     3841 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     3901 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     3961 tcctttgaaa aacacgatga taataccatg gctagattag ataaaagtaa agtgattaac
     4021 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
     4081 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
     4141 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     4201 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     4261 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     4321 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     4381 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     4441 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     4501 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     4561 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtcgcc aaaaaagaag
     4621 agaaaggtcg acggcggtgg tgctttgtct cctcagcact ctgctgtcac tcaaggaagt
     4681 atcatcaaga acaaggaggg catggatgct aagtcactaa ctgcctggtc ccggacactg
     4741 gtgaccttca aggatgtatt tgtggacttc accagggagg agtggaagct gctggacact
     4801 gctcagcaga tcgtgtacag aaatgtgatg ctggagaact ataagaacct ggtttccttg
     4861 ggttatcagc ttactaagcc agatgtgatc ctccggttgg agaagggaga agagccctgg
     4921 ctggtggaga gagaaattca ccaagagacc catcctgatt cagagactgc atttgaaatc
     4981 aaatcatcag tttaaggatc tcgactagct agtagctagc tagctagtcg agctcaagct
     5041 tcgaattcga tatcaagctt atcgcgatac cgtcgacctc gactagtcat atgataatca
     5101 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5161 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5221 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5281 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5341 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5401 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5461 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     5521 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5581 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5641 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggt acgtatggcc
     5701 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5761 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctc gagtttacca
     5821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     5881 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     5941 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     6001 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     6061 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcggatcg atctgctttt
     6121 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6181 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6241 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6301 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     6361 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     6421 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     6481 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     6541 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     6601 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     6661 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     6721 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     6781 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     6841 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     6901 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6961 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7021 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7081 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7141 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7201 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7261 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7321 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7381 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7441 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7501 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7561 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7621 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7681 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7741 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7801 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7861 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7921 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7981 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8041 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8101 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8161 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8221 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8281 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8341 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8401 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8461 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8521 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8581 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8641 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8701 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8761 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8821 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8881 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8941 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9001 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9061 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     9121 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9181 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9241 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9421 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     9481 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     9541 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     9601 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     9661 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     9721 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     9781 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     9841 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     9901 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     9961 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    10021 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    10081 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    10141 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    10201 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    10261 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    10321 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    10381 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    10441 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    10501 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    10561 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    10621 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    10681 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    10741 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    10801 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
    10861 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
    10921 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
    10981 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
    11041 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
    11101 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    11161 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    11221 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11281 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    11341 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    11401 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    11461 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtag
//
