LOCUS       sequence-445009-        5553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  promoter activity assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma Z, Qin H, Benveniste EN
  TITLE     Transcriptional suppression of matrix metalloproteinase-9 gene 
            expression by IFN-gamma and IFN-beta: critical role of STAT-1alpha.
  JOURNAL   J Immunol. 2001 Nov 1;167(9):5150-9.
  PUBMED    11673527
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2001-11-1"; volume: "167(9)"; pages: "5150-9"
COMMENT     Sequence Label: human MMP-9 promoter
FEATURES             Location/Qualifiers
     source          1..5553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    74..122
                     /note="synthetic polyadenylation signal"
     misc_feature    136..227
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     176..195
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      1051..1060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1057..2709
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1123..1141)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2750..2871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2761..2780)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2815..2834
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3119..3136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3290..3878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3370..3389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4049..4840,4841..4909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4672..4691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4910..5014)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5041..5496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5128..5147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5338..5359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac
       61 ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
      121 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
      181 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc gataggtacc
      241 gagctcttac gcgtgctagc ccctagaggc tgctactgtc ccctttactg ccctgaagat
      301 tcagcctgcg gaagacaggg ggttgcccca gtggaattcc ccagccttgc ctagcagagc
      361 ccattccttc cgcccccaga tgaagcaggg agaggaagct gagtcaaaga aggctgtcag
      421 ggagggaaaa agaggacaga gcctggagtg tggggagggg tttggggagg atatctgacc
      481 tgggaggggg tgttgcaaaa ggccaaggat gggccagggg gatcattagt ttcagaaaga
      541 agtctcaggg agtcttccat cactttccct tggctgacca ctggaggctt tcagaccaag
      601 ggatggggga tccctccagc ttcatccccc tccctccctt tcatacagtt cccacaagct
      661 ctgcagtttg caaaacccta cccctcccct gagggcctgc ggtttcctgc gggtctgggg
      721 tcttgcctga cttggcagtg gagactgcgg gcagtggaga gaggaggagg tggtgtaagc
      781 cctttctcat gctggtgctg ccacacacac acacacacac acacacacac acacacacac
      841 accctgaccc ctgagtcagc acttgcctgt caaggagggg tggggtcaca ggagcgcctc
      901 cttaaagccc ccacaacagc agctgcagtc agacacctct gccctcacca tgagtctctg
      961 gcagcccctg gtcctggtgc tcctggggat cctctagggg ctcgagatct gcgatctaag
     1021 taagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag
     1081 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
     1141 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
     1201 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
     1261 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
     1321 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
     1381 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
     1441 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
     1501 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
     1561 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
     1621 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
     1681 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
     1741 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
     1801 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
     1861 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg
     1921 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat
     1981 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc
     2041 aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct
     2101 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
     2161 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc
     2221 gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc
     2281 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa
     2341 gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag
     2401 gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt
     2461 gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
     2521 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
     2581 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
     2641 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc
     2701 gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
     2761 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2821 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2881 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     2941 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc
     3001 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     3061 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     3121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5041 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5101 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5161 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5221 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5281 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5341 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5401 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5461 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
     5521 actgttggga agggcgatcg gtgcgggcct ctt
//
