LOCUS       sequence-44526-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6640 MSCV-P C-FlagHA 16E7-Kozak
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..89
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     105..121
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     149..168
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        149..167
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    197..221
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(516..540)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             572..595
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             611..637
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             611..637
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(685..708)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccctctgggt agccctttgt caccctaagc ctccgcctcc tcttcctcca tccgccccgt
       61 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
      121 ctccttctct aggcgccgga attagatcta atacgactca ctatagggag acccaagctg
      181 gctagttaag ctatcaacaa gtttgtacaa aaaagttggc accatgcatg gagatacacc
      241 tacattgcat gaatatatgt tagatttgca accagagaca actgatctct actgttatga
      301 gcaattaaat gacagctcag aggaggagga tgaaatagat ggtccagctg gacaagcaga
      361 accggacaga gcccattaca atattgtaac cttttgttgc aagtgtgact ctacgcttcg
      421 gttgtgcgta caaagcacac acgtagacat tcgtactttg gaagacctgt taatgggcac
      481 actaggaatt gtgtgcccca tctgttctca gaaaccacca actttcttgt acaaagtggt
      541 tgatctagag ggcccgcggt tcgaactcga ggactacaag gacgacgatg acaagtcggc
      601 cgctggagga tacccctacg acgtgcccga ctacgcctag aattctaccg ggtaggggag
      661 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      721 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      781 ccgtttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
      841 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
      901 tctcgtgcag atggacagca ccgctgagca atgg
//
