LOCUS       sequence-445289-        8728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Make lentivirus to express mCherry in syn1 expressing cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Sirois CL, Guo Y, Li M, Wolkoff NE, Korabelnikov T, Sandoval S, Lee 
            J, Shen M, Contractor A, Sousa AMM, Bhattacharyya A, Zhao X
  TITLE     CGG repeats in the human FMR1 gene regulate mRNA localization and 
            cellular stress in developing neurons.
  JOURNAL   Cell Rep. 2024 Jun 11;43(6):114330. doi: 
            10.1016/j.celrep.2024.114330.
  PUBMED    38865241
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-06-11"; volume: "43(6):114330. doi"; pages: " 
            10.1016/j.celrep.2024.114330"
COMMENT     Sequence Label: pLV-Syn1-mCherry
FEATURES             Location/Qualifiers
     source          1..8728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..91
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    224..448
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     437..454
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             480..713
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    785..1373
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             891..902
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1300..1320
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1379..2089)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1657..1676)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1926..1944
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2086..2095
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2122..2569)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    2636..2753
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3051..3095)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3280..3513
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4006..4131
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4178..4358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4376..4574)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4401..4421)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4576..4955
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5127..5146
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5221..5325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5326..5394,5395..6186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5544..5563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6357..6945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6846..6865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7099..7116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7233..7254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7269..7274,7275..7292,7293..7299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7307..7323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7312..7334
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7331..7347
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7331..7347
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7396..7517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7407..7426)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7461..7480
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7647..8021)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(8040..8087)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(8135..8464)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8149..8284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8203..8222)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8449..8469
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(join(8478..8728,1..178))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8588..8609)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
       61 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
      121 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg
      181 ggataccccc tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca
      241 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
      301 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
      361 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
      421 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct
      481 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
      541 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
      601 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
      661 agtacaagca aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc
      721 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagt
      781 cgacgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc
      841 ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc
      901 gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc
      961 caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga
     1021 gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg
     1081 gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg
     1141 gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc
     1201 tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa
     1261 gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca
     1321 tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg attctcgatt
     1381 acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt
     1441 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg
     1501 cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt
     1561 ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg
     1621 ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt
     1681 cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg
     1741 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
     1801 tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt
     1861 aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc
     1921 cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
     1981 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
     2041 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatg gtggcgaccg
     2101 ggggatccgg taccttctcg actgcgctct caggcacgac acgactcctc cgctgcccac
     2161 cgcagactga ggcagcgctg agtcgccggc gccgcagcgc agatggtcgc gcccgtgccc
     2221 ccctatctcg cgcctcgcgt ggtgcggtcc ggctgggccg gcggcggcgc ggacgcgacc
     2281 aaggtggccg ggaaggggag tttgcggggg accggcgagt gacgtcagcg cgccttcagt
     2341 gctgaggcgg cggtggcgcg cgccgccagg cgggggcgaa ggcactgtcc gcggtgctga
     2401 agctggcagt gcgcacgcgc ctcgccgcat cctgtttccc ctccccctct ctgatagggg
     2461 atgcgcaatt tggggaatgg gggttgggtg cttgtccagt gggtcggggt cggtcgtcag
     2521 gtaggcaccc ccaccccgcc tcatcctggt cctaaaaccc acttgcactc atacgcaggg
     2581 ccctcggtac cttaattaac caaactggat ctctgctgtc cctgtaataa acccgaaaat
     2641 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     2701 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga
     2761 tgaatactgc catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga
     2821 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
     2881 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
     2941 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
     3001 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     3061 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     3121 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     3181 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     3241 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     3301 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     3361 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     3421 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     3481 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     3541 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     3601 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     3661 agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa
     3721 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     3781 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     3841 tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag
     3901 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     3961 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     4021 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     4081 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     4141 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     4201 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     4261 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     4321 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa acgcgctgct
     4381 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     4441 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     4501 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     4561 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     4621 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     4681 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     4741 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     4801 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     4861 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     4921 tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc
     4981 gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc
     5041 ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc
     5101 agggagcaga tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5161 taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg
     5221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6001 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6061 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6181 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6241 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7201 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7321 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7381 ggtatacaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     7441 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     7501 gctgcaataa acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     7561 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     7621 gtggaatcga aatctcgtag cacgtgtcag tcctgctcct cggccacgaa gtgcacgcag
     7681 ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc
     7741 atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac
     7801 agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc
     7861 tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc
     7921 acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg
     7981 cgcgcggtga gcaccggaac ggcactggtc aacttggcca tggtttagtt cctcaccttg
     8041 tcgtattata ctatgccgat atactatgcc gatgattaat tgtcaacacg tgctgatcag
     8101 atccgaaaat ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa
     8161 agcctcctca ctacttctgg aatagctcag aggcagaggc ggcctcggcc tctgcataaa
     8221 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     8281 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     8341 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     8401 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     8461 acacattcca cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     8521 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     8581 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     8641 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     8701 tttggggtcg aggtgccgta aagcacta
//
