LOCUS       sequence-44536-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH
  TITLE     Real-time observation of transcription initiation and elongation on 
            an endogenous yeast gene.
  JOURNAL   Science. 2011 Apr 22;332(6028):475-8.
  PUBMED    21512033
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-04-22"; volume: "332(6028)"; pages: "475-8"
COMMENT     Sequence Label: pDZ276 (pURA MET28 PCP-2x-yeGFP)
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             114..827
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIKLKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 agtccctcgt cgcgacctcg caggtcgaag atcttgtcgt caaccttgtg ccgctgggcc
       61 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacatgtcta
      121 aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta
      181 atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga
      241 ccttaaaatt tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta
      301 ctttaactta tggtgttcaa tgtttttcta gatacccaga tcatatgaaa caacatgact
      361 ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg
      421 acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa
      481 tcaaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat
      541 acaactataa ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag
      601 ttaacttcaa aattagacac aacattgaag atggttctgt tcaattagct gaccattatc
      661 aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcca
      721 ctcaatctgc cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat
      781 ttgttactgc tgctggtatt acccatggta tggatgaatt gtacaaaaga tcccccgggc
      841 tgcaggaatt cgatatcaag cttatcgata cc
//
