LOCUS       sequence-445470-        7293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpR, GvpN and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaRNS
FEATURES             Location/Qualifiers
     source          1..7293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2012..2029
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5551..5569)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5565..5612
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5679..5770
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6635..6674
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6705..7293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7194..7213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag ctttaggagc
     1441 ttgaaaacat gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg
     1501 ataaagacga agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga
     1561 ttcgctacaa agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta
     1621 atcgccaaaa tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata
     1681 cggttattcc aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt
     1741 tggaaaacct ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag
     1801 tcggtttaaa agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg
     1861 aacttgagaa agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc
     1921 gtattcaact tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga
     1981 cagatgtgtt ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg
     2041 gcgaaacgat gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg
     2101 atgaaaaagt aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg
     2161 gtccttggcc tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg
     2221 cttcacaaat tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag
     2281 gaagaagctg ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt
     2341 caaatgatgt ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa
     2401 ttgttaatga ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca
     2461 caaaaaagaa atgaggaatc ctagatggga gaattactgt atttatacgg tttaattcca
     2521 acaaaagaag cagcagccat agagccgttt ccatcttata aggggtttga cggagaacat
     2581 tcactgtacc caattgcgtt tgatcaggtg acggctgtag tttctaagct ggatgctgac
     2641 acctattcag aaaaagtgat tcaagaaaaa atggagcagg atatgagctg gctgcaggaa
     2701 aaagcatttc atcatcacga aacggtagcc gctttgtacg aagaatttac gatcattcca
     2761 ttaaaatttt gcaccattta taaaggtgaa gaaagtctgc aggcagctat tgagattaac
     2821 aaagaaaaga tagagaattc actgacgctg cttcaaggaa atgaagagtg gaatgtgaaa
     2881 atttactgtg atgatacaga gcttaaaaaa ggaatcagcg aaacgaatga aagcgtgaaa
     2941 gcgaaaaaac aagaaattag tcacttatca ccaggaagac agttttttga aaagaaaaaa
     3001 atagatcagc tgattgaaaa agaattagag cttcacaaaa acaaagtgtg tgaagagata
     3061 catgacaagc taaaagaatt atcgctttat gactctgtta aaaagaattg gagcaaagac
     3121 gtaactggcg cagctgaaca gatggcgtgg aacagcgtgt ttcttctccc gtctctgcag
     3181 attactaagt tcgtaaacga aatagaagag cttcagcaaa ggcttgaaaa taaaggctgg
     3241 aagtttgaag tgacgggacc atggccgccc tatcatttct cgagctttgc gtaaaagcgg
     3301 ttaagcgtga cagtccagga ggaattaatg gatgcaaccg gtcagccaag caaatggacg
     3361 aatccacttg gatcctgatc aagctgaaca aggcttagcg cagcttgtga tgacagttat
     3421 tgagctattg aggcaaatcg ttgaacgtca tgccatgagg cgggtggagg gtggaacgtt
     3481 gacggacgaa caaattgaaa acttaggaat tgcactaatg aacttagaag aaaaaatgga
     3541 cgagttgaaa gaggtgttcg gtctggatgc agaagattta aatattgatc ttggaccgct
     3601 aggcagcctg ctttaagcgg tcagtaggag gaacagtatg gcagtcgaac ataatatgca
     3661 gtcaagtacg attgtagatg tgctcgaaaa gattttggat aaaggagtcg ttatagcggg
     3721 ggacatcacc gtaggaattg cagatgtcga gctattaacg ataaagatcc gcttgattgt
     3781 ggcttcggtt gataaggcaa aagaaatcgg catggactgg tgggaaaatg atccgtatct
     3841 cagttcaaaa ggagccaata acaaagcgct cgaagaagaa aataaaatgc tgcatgagcg
     3901 gttaaaaacg cttgaagaaa aaatagaaac gaaacgttaa aaactgtacg ctacttaaaa
     3961 aatggaggga tttacaatgg caactgaaac aaaattagat aacacacagg cagaaaacaa
     4021 ggaaaataaa aatgcggaaa acggttcaaa agaaaagaac ggttcaaaag caagcaaaac
     4081 aacaagcagc gggccaatca aacgagcggt agcaggaggc atcatcggtg caacgattgg
     4141 atatgtatcg actcctgaaa atcgaaaaag tctccttgac cgcattgata cagacgaatt
     4201 aaaaagcaaa gcatctgatt taggaacaaa ggtaaaagaa aaatcaaaaa gcagcgtggc
     4261 cagcctgaaa acatctgcgg gaagcttgtt taaaaaagat aaagataaat caaaagatga
     4321 tgaagaaaac gtaaattctt ctagtagcga aacagaagac gataacgttc aagagtacga
     4381 cgagttaaaa gaagaaaatc aaactcttca agatcgctta tcacagcttg aagaaaaaat
     4441 gaacatgctt gttgagctta gcctcaataa aaatcaagac gaagaagcgg aagatacaga
     4501 ttccgacgaa gaagagaacg atgagaacga tgaaaacgat gaaaacgagc aggacgatga
     4561 aaacgaagaa gaaacatcta agccacgtaa aaaggataaa aaagaagctg aggaagaaga
     4621 aagtgaaagt gacgaagaca gcgaggaaga agaggaagat tctcgctcaa acaaaaaaaa
     4681 taaaaaagta aaaacagaag aagaagacga agatgaaagc gaagaagaaa aaaaggaagc
     4741 gaaaccaaaa aagtcaacag ctaaaaaatc aaaaaataca aaagcaaaga aaaacacgga
     4801 cgaagaagat gatgaagcaa catctctttc tagtgaagac gatacaacag cctaagacgt
     4861 aaaggaggaa agaaagacat gagtacaggc ccttcttttt caactaaaga caatacgctt
     4921 gaatactttg tgaaagcttc taataaacac ggcttttcac ttgatatttc attaaatgta
     4981 aacggcgctg tgatttccgg taccatgatt tcagcaaaag aatactttga ttacttaagc
     5041 gaaacgtttg aagaaggcag tgaagtggct caggcgctaa gcgaacaatt ctctttagca
     5101 agcgaagcga gcgaatcaaa cggagaagca gaagcccatt ttattcattt gaaaaataca
     5161 aagatttact gtggagacag taaatctact ccttctaaag gcaaaatctt ttggagaggg
     5221 aaaatagcag aagtagacgg gtttttctta ggaaagattt ctgatgcaaa atcaacgagt
     5281 aaaaagagtt cataaaaaac ggcggggtga ttgccccgcc gttttttagt gatgtgatga
     5341 gatgtgcagc ttcttttttc gatacatgag cggagtcatc ttcatttctt ttcgaaactg
     5401 attaataaaa tagcttgtac tattaaaccc tacttgatag gcgacttccg tcacatttgc
     5461 ttctgcttgc tgcagtccgg attcgcgaac gcgtctcgag agatccggct gctaacaaag
     5521 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     5581 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccgactagt
     5641 agcccgccta atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca
     5701 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5761 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5821 ttgccttcct gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca
     5881 attgggggca cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag
     5941 ttttcgccct gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc
     6001 agtattatcc cgtgtagatg cggggcaaga gcaactcgga cgacgaatac actattcgca
     6061 gaatgacttg gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt
     6121 aagagaatta tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct
     6181 gacaaccatc ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt
     6241 aactcgcctt gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga
     6301 caccacaatg cctgcggcaa tggcaacaac attacgcaaa ctattaactg gcgaactact
     6361 tactctggct tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc
     6421 actactgcgt tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga
     6481 gcgtggttca cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt
     6541 agttatctac acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga
     6601 aataggggct tcactgatta agcattggta aaccgataca attaaaggct ccttttggag
     6661 cctttttttt tggacggacc ggtagaaaag atcaaaggat cttcttgaga tccttttttt
     6721 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6781 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6841 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6901 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6961 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7021 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7081 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7141 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7201 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7261 tgatgctcgt caggggggcg gagcctatgg aaa
//
