LOCUS       sequence-445475-        3989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   de Moura Gomes A, L Petkau T, J Korecki A, Fornes O, Galvan A, Lu G,
            M Hill A, Ling Lam S, Yao A, A Farkas R, W Wasserman W, Smith Y, M 
            Simpson E, R Leavitt B
  TITLE     New MiniPromoter Ple389 (ADORA2A) drives selective expression in 
            medium spiny neurons in mice and non-human primates.
  JOURNAL   Sci Rep. 2024 Nov 15;14(1):28194. doi: 10.1038/s41598-024-79004-y.
  PUBMED    39548191
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-11-15"; volume: "14(1):28194. doi"; pages: " 
            10.1038/s41598-024-79004-y"
COMMENT     Sequence Label: pEMS2372
FEATURES             Location/Qualifiers
     source          1..3989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..24
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     21..37
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     21..37
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1410..1427)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1410..1426)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1419..1441)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1635..1654)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1754..1776
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1814..1832)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1900..2004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2005..2073,2074..2865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2223..2242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3036..3624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3525..3544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3778..3795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3912..3933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3948..3953,3954..3971,3972..3978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(3986..3989,1..13)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgccg gcgcgccccc
       61 cccatgctca gcctctcagg cctcctgtgg cagcttggcc agtctcctgg gtcctggatc
      121 tcggccagcc ccatgacact ccctcccaga aaggcccctc cctgcccagg ctctcccagt
      181 cccacccacc ttctctcgcc aggccaactt ctcagccccc ctggcctgct ccatcccgtc
      241 aactctccca ggaactacca gcacccacct aagggacacc agccgccctg tgaaaggaca
      301 agccagagac ccctggagct cggatgtgga aggatgtggg gagctgcctg gtcctgtctc
      361 ccactgccgc agagggtaag ccctctcata ctgcgcccga acccgctgcc tccaggtgcc
      421 cctgccagtc agctgcagtc agggcagggg cggcgctgca gctgactgtg agcttccact
      481 cggagggagc tgcctcacct ccctcctccc acgccctctc ccccacctgc tcctctgcct
      541 ggctgacacc aggaatgact aacgggagtt tggtccctgg cagaaagcag gtacggaacc
      601 aggcttggcc taaggcccgc ctctggccag aggtgactgg gctgcccctg gcttcctgag
      661 gcaaggagca ggaaagctca cagctcagcc agctcagcac tggggaaaca ggggacacgg
      721 atggaggggt agtggctggg atttgcctga gaaactctag gactagggag aggctgggag
      781 accccaaaaa gcaagcgcag aacaaggagg gactgacacc gtggccgtgg acggtcagtt
      841 ctgaatccaa acagtaaccg gacaagccca ttctcaggag ctcctcagct ccattccgtg
      901 ggggcttggt tacctgtaac agggcctcaa caggagggtc atagacttgc ccattgcttc
      961 caagcaacag ggagttggca ttgccctgcc tcctgtggga ctcgcaaaca taaaatctca
     1021 ggctctctca ttctcacatc tacattacat agcactgact ctgtgccgga cactgttctt
     1081 agtgctttgc aaatagtaat ttatatagtc ctcattgact gggaagctat attaattttg
     1141 acattattcc tgagaaaagc atatcccttc ctgggttctg gccctgttaa gaaatctctg
     1201 aatataaacc aggctctatc cccctcactc ccctttccaa cctaactaga aaatgtgggt
     1261 gggttgagaa agaacagaaa agaaagataa aaactgtgac agccttctga caacatttta
     1321 acttgaaaat tgcctccaaa tggatggcat aattcagtgt aggcagatga aaatcaggcg
     1381 cagatttact cagcccctcc tggccggcca ctggccgtcg ttttacaacg tcgtgactgg
     1441 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1561 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     1621 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     1681 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     1741 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     1801 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1861 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1921 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1981 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2041 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2101 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2161 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2221 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2281 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2341 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2401 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2461 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2521 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2581 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2641 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2701 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2761 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2821 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2881 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2941 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3001 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3061 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3901 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3961 cttccggctc gtatgttgtg tggaattgt
//
