LOCUS       sequence-445478-        3738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High Copy number plasmid containing Ple384 (TH MiniPromoter) insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   n/a
  TITLE     TH MiniPromoter Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pEMS2367
FEATURES             Location/Qualifiers
     source          1..3738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      542..1130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1031..1050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1284..1301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1418..1439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1454..1459,1460..1477,1478..1484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1492..1508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1497..1519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1516..1532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1516..1532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2654..2671)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2654..2670)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2663..2685)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2879..2898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2998..3020
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3058..3076)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3144..3248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3249..3317,3318..3738,1..371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3467..3486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
       61 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     1321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     1441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1501 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt gcatgcaggc
     1561 ctctgcagtc gacgggcccg ggatccgatg gcgcgccgga ggtccgggct ccgtctccac
     1621 agccctggcc cagcagcctc ttataagctg agctgacgtc aaagccccct ctgggtcccc
     1681 caccttcccc tccttacatc ccccacccct gcctgctgtg cctgatggag gcgggggtgg
     1741 gtgaggaagg agggaggcgc aggagaaagg gctcgtccgt ggaatctaat tgccttcact
     1801 ccccagccct gtctgaatca tcctcagccc gcgggcttgc ggggaccagg ggctcgtgct
     1861 ctgcgggggg ctgagaccag ggtcggctgc tgaggatccc tcggtgaggc agctccaggc
     1921 cccccagcac cccgccccag ctctgcccag ggcggcccca gtgctcaggt gcccaggcca
     1981 ggaggagaga catgcctgat gaacaccacg ccggaggctc atggcccaca ctgccggatg
     2041 tggatttaac ctttcagcaa atgtcttcca ctgatacaaa tattgctttt taattcggtt
     2101 tcctgccggg ccagttgtgc tttcatggaa atggagcgtg ttctctctca acagcctctc
     2161 agcccgaggc ttaatcaccc agattaatca cccagcacgt ctgtgccctg ggactggtgc
     2221 ccactccccg cgtggggcag ggtcagagtg ctgacttcga cctggctgtg aggctgctcc
     2281 gcccacaggg cgagtctctg aggccaccag gccgcattca cttcaggtac ctctgctaat
     2341 cccggggctc ggaggagccc ccacccccgg ctgacatttc caggccgctg cgtgccgctg
     2401 ccccagccgc cgtgatattt agacagggct tgtttgccat gtgattactt tggggcctgg
     2461 gatgaactct accaacacct gagcttgtcc gctttgctcc aaagcttgtt tttaatacac
     2521 tcgctgtcag ctaagccggc tccctccagc tgtggggtcc ggtcccctcc tgggccagag
     2581 ggaggactaa gcccaggttt tcctcaggag gccggccatc tagatgcatt cgcgaggtac
     2641 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2701 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2761 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     2821 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     2881 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     2941 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3001 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     3061 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3721 ctcgccttga tcgttggg
//
