LOCUS       sequence-445535-        7328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpR, GvpL and GvpJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaRLJ
FEATURES             Location/Qualifiers
     source          1..7328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2955..2972
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5586..5604)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5600..5647
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5714..5805
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6670..6709
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6740..7328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7229..7248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag ctttcaggag
     1441 gaacgtaaaa atgaccgtct taacagacaa aaggaaaaaa ggcagtggag cttttataca
     1501 agatgacgag acaaaagagg ttctttcaag agcgctgagc tatttaaaat ccggctattc
     1561 cattcatttt acaggtcctg ccggcggagg caaaacctct ttagcgcgag cgcttgctaa
     1621 aaagagaaag cgtcctgtaa tgctgatgca cgggaatcac gagctcaaca acaaagattt
     1681 aattggcgat tttacgggat acacgagcaa aaaagtaatc gaccagtacg ttcgttctgt
     1741 ctataaaaaa gatgaacagg tgagtgaaaa ctggcaggat ggccgattgc ttgaagctgt
     1801 aaaaaatggc tatacgctga tttacgacga atttactcgt tctaagcctg cgacgaataa
     1861 tatctttcta tcgatattag aagaaggcgt gctgccgctg tatggagtaa aaatgaccga
     1921 tccttttgtg cgcgtgcatc ccgatttccg cgtcatcttc acaagcaatc cagctgagta
     1981 tgccggcgta tatgatacgc aagatgcgct tctcgacagg ttaattacca tgtttattga
     2041 ttataaagac atcgacagag agacagcgat tttaacggag aaaacggacg tagaagaaga
     2101 tgaagcgcgc acaattgtaa cgctcgtagc aaacgtgcga aaccgctctg gagacgaaaa
     2161 cagcagcgga cttagcctgc gggcttcgct tatgatcgct acccttgcca cgcagcaaga
     2221 cattcctatc gatggaagtg acgaagattt tcaaacgtta tgtatcgata ttttgcatca
     2281 tccgcttacc aaatgtttgg atgaagaaaa tgcaaaaagc aaagccgaaa aaatcatttt
     2341 agaagaatgt aagaatatag acactgaaga aaagtaaagg agcttgaaaa catgagtgaa
     2401 acaaacgaaa caggtattta tatttttagc gccattcaaa cggataaaga cgaagaattt
     2461 ggcgccgtgg aagtagaagg aacaaaagct gaaacatttt tgattcgcta caaagacgcg
     2521 gctatggtag cagctgaagt accgatgaaa atttatcatc ctaatcgcca aaatttatta
     2581 atgcatcaaa acgcagtagc agcgattatg gacaagaacg atacggttat tccaatcagc
     2641 tttgggaatg tattcaaatc aaaagaagac gtaaaagttc ttttggaaaa cctttatccg
     2701 cagtttgaaa agctgtttcc agcgatcaaa ggaaaaattg aagtcggttt aaaagtaatt
     2761 gggaaaaaag aatggcttga gaaaaaagta aacgaaaatc ctgaacttga gaaagtatca
     2821 gcatccgtaa aaggaaaatc agaagcagcc ggttattatg agcgtattca acttggagga
     2881 atggctcaaa agatgtttac ttccctgcaa aaagaagtca agacagatgt gttttctccg
     2941 cttgaagaag cagcggaagc agcaaaagca aatgagccaa cgggcgaaac gatgctttta
     3001 aacgcgtctt tcttaattaa ccgagaagat gaagcgaagt ttgatgaaaa agtaaatgaa
     3061 gcgcatgaaa actggaaaga caaagccgat tttcattaca gcggtccttg gcctgcttat
     3121 aattttgtga acattcgcct aaaagtagaa gagaaataac gtgcttcaca aattagtaac
     3181 cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta caggaagaag ctgataaaca
     3241 gctatatgac cttccgacga ttcagcaaaa gctcattcag cttcaaatga tgtttgagct
     3301 tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat gaattgttaa tgaggtacga
     3361 aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta acacaaaaaa gaaatgagga
     3421 atcctagagt gaggaattaa cattatgtct cttaaacaat ccatggagaa taaagatatt
     3481 gctcttattg atattttaga tgtcatttta gataaaggag tcgccattaa aggagactta
     3541 atcatttcca tagctggcgt cgatttagtg tatttggatt tgcgggtgct tatttcttcg
     3601 gttgaaacgc ttgtgcaagc aaaagaagga aatcacaaac caatcacttc tgaacaattt
     3661 gataaacaaa aggaggaatt aatggatgca accggtcagc caagcaaatg gacgaatcca
     3721 cttggatcct gatcaagctg aacaaggctt agcgcagctt gtgatgacag ttattgagct
     3781 attgaggcaa atcgttgaac gtcatgccat gaggcgggtg gagggtggaa cgttgacgga
     3841 cgaacaaatt gaaaacttag gaattgcact aatgaactta gaagaaaaaa tggacgagtt
     3901 gaaagaggtg ttcggtctgg atgcagaaga tttaaatatt gatcttggac cgctaggcag
     3961 cctgctttaa gcggtcagct gtacgctact taaaaaatgg agggatttac aatggcaact
     4021 gaaacaaaat tagataacac acaggcagaa aacaaggaaa ataaaaatgc ggaaaacggt
     4081 tcaaaagaaa agaacggttc aaaagcaagc aaaacaacaa gcagcgggcc aatcaaacga
     4141 gcggtagcag gaggcatcat cggtgcaacg attggatatg tatcgactcc tgaaaatcga
     4201 aaaagtctcc ttgaccgcat tgatacagac gaattaaaaa gcaaagcatc tgatttagga
     4261 acaaaggtaa aagaaaaatc aaaaagcagc gtggccagcc tgaaaacatc tgcgggaagc
     4321 ttgtttaaaa aagataaaga taaatcaaaa gatgatgaag aaaacgtaaa ttcttctagt
     4381 agcgaaacag aagacgataa cgttcaagag tacgacgagt taaaagaaga aaatcaaact
     4441 cttcaagatc gcttatcaca gcttgaagaa aaaatgaaca tgcttgttga gcttagcctc
     4501 aataaaaatc aagacgaaga agcggaagat acagattccg acgaagaaga gaacgatgag
     4561 aacgatgaaa acgatgaaaa cgagcaggac gatgaaaacg aagaagaaac atctaagcca
     4621 cgtaaaaagg ataaaaaaga agctgaggaa gaagaaagtg aaagtgacga agacagcgag
     4681 gaagaagagg aagattctcg ctcaaacaaa aaaaataaaa aagtaaaaac agaagaagaa
     4741 gacgaagatg aaagcgaaga agaaaaaaag gaagcgaaac caaaaaagtc aacagctaaa
     4801 aaatcaaaaa atacaaaagc aaagaaaaac acggacgaag aagatgatga agcaacatct
     4861 ctttctagtg aagacgatac aacagcctaa gacgtaaagg aggaaagaaa gacatgagta
     4921 caggcccttc tttttcaact aaagacaata cgcttgaata ctttgtgaaa gcttctaata
     4981 aacacggctt ttcacttgat atttcattaa atgtaaacgg cgctgtgatt tccggtacca
     5041 tgatttcagc aaaagaatac tttgattact taagcgaaac gtttgaagaa ggcagtgaag
     5101 tggctcaggc gctaagcgaa caattctctt tagcaagcga agcgagcgaa tcaaacggag
     5161 aagcagaagc ccattttatt catttgaaaa atacaaagat ttactgtgga gacagtaaat
     5221 ctactccttc taaaggcaaa atcttttgga gagggaaaat agcagaagta gacgggtttt
     5281 tcttaggaaa gatttctgat gcaaaatcaa cgagtaaaaa gagttcataa aaaacggcgg
     5341 ggtgattgcc ccgccgtttt ttagtgatgt gatgagatgt gcagcttctt ttttcgatac
     5401 atgagcggag tcatcttcat ttcttttcga aactgattaa taaaatagct tgtactatta
     5461 aaccctactt gataggcgac ttccgtcaca tttgcttctg cttgctgcag tccggattcg
     5521 cgaacgcgtc tcgagagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     5581 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     5641 ttttttgctg aaaggaggaa ctatatccga ctagtagccc gcctaatgag cgggcttttt
     5701 tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5761 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5821 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5881 gaaacgctcg tgaaagtaaa agacgcagag gaccaattgg gggcacgagt gggatacata
     5941 gaactggact tgaatagcgg taaaatcctt gagagttttc gccctgaaga gcgttttcca
     6001 atgatgagca ctttcaaagt tctgctatgt ggagcagtat tatcccgtgt agatgcgggg
     6061 caagagcaac tcggacgacg aatacactat tcgcagaatg acttggttga atactcccca
     6121 gtgacagaaa agcaccttac ggacggaatg acggtaagag aattatgtag tgccgccata
     6181 acgatgagtg ataacactgc ggcgaactta cttctgacaa ccatcggtgg accgaaggaa
     6241 ttaaccgctt ttttgcacaa tatgggagac catgtaactc gccttgaccg ttgggaacca
     6301 gaactgaatg aagccatacc aaacgacgag cgagacacca caatgcctgc ggcaatggca
     6361 acaacattac gcaaactatt aactggcgaa ctacttactc tggcttcacg gcaacaatta
     6421 atagactggc ttgaagcgga taaagttgca ggaccactac tgcgttcggc acttcctgct
     6481 ggctggttta ttgctgataa atctggggca ggagagcgtg gttcacgggg tatcattgcc
     6541 gcacttggac cagatggtaa gccttcccgt atcgtagtta tctacacgac gggtagtcag
     6601 gcaactatgg acgaacgaaa tagacagatt gctgaaatag gggcttcact gattaagcat
     6661 tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac ggaccggtag
     6721 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6781 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6841 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6901 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6961 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7021 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7081 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7141 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7201 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7261 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7321 tatggaaa
//
