LOCUS       sequence-445614-        2639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged ATG3 (K208D) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, 
            Hummer G
  TITLE     Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the 
            phagophore.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. 
            Epub 2024 Feb 7.
  PUBMED    38324698
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj8027. doi"; pages: " 
            10.1126/sciadv.adj8027. Epub 2024 Feb 7"
COMMENT     Sequence Label: pMX-IG-ATG3 (K208D)
FEATURES             Location/Qualifiers
     source          1..2639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      671..1259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1160..1179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1413..1430
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             1560..2029
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        2181..2285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2286..2354,2355..2639,1..507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2504..2523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
       61 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      361 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
      541 attgatttgc ggccggccgc aaaaggatct aggtgaagat cctttttgat aatctcatga
      601 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      661 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      721 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      781 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      841 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      901 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      961 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1021 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1081 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1141 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1201 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1261 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1321 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1381 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1441 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
     1501 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
     1561 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
     1621 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
     1681 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
     1741 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
     1801 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
     1861 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
     1921 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
     1981 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
     2041 gttcctgacc ttgatctgaa cttttctatt ctcagttatg tatttttcca tgccttgcaa
     2101 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tatgcaggtg
     2161 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2221 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2281 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2341 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2401 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2461 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2521 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2581 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaaga
//
