LOCUS       sequence-445617-        2638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged ATG3 (T244A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, 
            Hummer G
  TITLE     Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the 
            phagophore.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. 
            Epub 2024 Feb 7.
  PUBMED    38324698
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj8027. doi"; pages: " 
            10.1126/sciadv.adj8027. Epub 2024 Feb 7"
COMMENT     Sequence Label: pMX-IG-ATG3 (T244A)
FEATURES             Location/Qualifiers
     source          1..2638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        117..221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(222..290,291..1082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(440..459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1246..1834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1735..1754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1988..2005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             2135..2604
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tctattctca gttatgtatt tttccatgcc ttgcaaaatg gcgttactta agctagcttg
       61 ccaaacctac aggtggggtc tttcattatg caggtggcac ttttcgggga aatgtgcgcg
      121 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      181 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      241 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      301 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      361 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      421 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      481 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      541 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      601 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      661 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      721 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
      781 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      841 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
      901 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
      961 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1021 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1081 aactgtcaga ccaagtttac tcatatatac tttagattga tttgcggccg gccgcaaaag
     1141 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1381 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1921 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1981 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2041 ccccgcgcgt tggccgattc attaatgcaa ctatggccat ttaatgtaaa tacttaagaa
     2101 aaaaaaccaa attaattttg atacatgctg catgtgaaag acccccgctg acgggtagtc
     2161 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
     2221 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc
     2281 gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc gaacagaagc
     2341 gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg tccttggggc
     2401 accctggaaa catctgatgg ttctctagaa actgctgagg gctggaccgc atctggggac
     2461 catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc
     2521 catattcagc tgttccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag
     2581 atatcctgtt tggcccatat tcagctgttc catctgttcc tgaccttgat ctgaactt
//
