LOCUS       sequence-445895-        8543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaS
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     406..425
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        406..424
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2813..2830
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6352..6370)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6366..6413
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6480..6571
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7436..7475
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7506..8094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7995..8014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8248..8265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8280..8419
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8345..8364)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8505..8527
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(8521..8543,1..169))
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
       61 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
      121 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
      181 tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgagaga ggatgctcac
      241 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
      301 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
      361 taatacagat gtaggtgttg gcaccatgca tcgatcccgc gaaattaata cgactcacta
      421 tagggagacc acaacggttt ccctctagag atagcttagc gatgtgaaag aagctggaga
      481 aaactttcaa gaaaaagttt cttcttcaga cgataaagag aagtcaaaaa ataagcggaa
      541 aataaaaggc gtaaatcaga ttaaaaaagc aacggatatt aaaagctcta ctaagattaa
      601 aagttctaat gatattaaat catctacaga tttaaaaaca atgggatcat aagcgaaagg
      661 agaaatgaga tatgtctatt caaaaaagta ctaatagttc aagtttagca gaagtcattg
      721 accgtatttt agataaagga attgttattg atgcttttgc aagagtttct gttgtaggaa
      781 ttgaaatttt aacgattgaa gcgcgagtgg ttattgccag tgttgataca tggttacgct
      841 atgcagaagc agtagggctt cttcgtgacg acgtagaaga aaacggtctt cctgaacgtt
      901 caaattcaag tgaagggcag ccgcgtttta gtatttaata tgaagtggta tggaaccttc
      961 cttttttgag ggtggttcca gcttttagat ctaactattg gaggctacta aaaatggaaa
     1021 ttaaaaaaat tatgcaagcc gtgaacgact ttttcggtga acacgtagct cctcctcata
     1081 aaattacctc ggtggaagct actgaagatg aaggttggag agttattgtt gaagtcattg
     1141 aagaacgaga atatatgaaa aaatacgcca aagatgaaat gctcggaacg tacgagtgct
     1201 ttgtaaataa agaaaaagaa gtcatttcat tcaaacgact cgacgtcaga tatagaagcg
     1261 ccattggcat tgaagcataa acgataagat ggcaggagga acgtaaaaat gaccgtctta
     1321 acagacaaaa ggaaaaaagg cagtggagct tttatacaag atgacgagac aaaagaggtt
     1381 ctttcaagag cgctgagcta tttaaaatcc ggctattcca ttcattttac aggtcctgcc
     1441 ggcggaggca aaacctcttt agcgcgagcg cttgctaaaa agagaaagcg tcctgtaatg
     1501 ctgatgcacg ggaatcacga gctcaacaac aaagatttaa ttggcgattt tacgggatac
     1561 acgagcaaaa aagtaatcga ccagtacgtt cgttctgtct ataaaaaaga tgaacaggtg
     1621 agtgaaaact ggcaggatgg ccgattgctt gaagctgtaa aaaatggcta tacgctgatt
     1681 tacgacgaat ttactcgttc taagcctgcg acgaataata tctttctatc gatattagaa
     1741 gaaggcgtgc tgccgctgta tggagtaaaa atgaccgatc cttttgtgcg cgtgcatccc
     1801 gatttccgcg tcatcttcac aagcaatcca gctgagtatg ccggcgtata tgatacgcaa
     1861 gatgcgcttc tcgacaggtt aattaccatg tttattgatt ataaagacat cgacagagag
     1921 acagcgattt taacggagaa aacggacgta gaagaagatg aagcgcgcac aattgtaacg
     1981 ctcgtagcaa acgtgcgaaa ccgctctgga gacgaaaaca gcagcggact tagcctgcgg
     2041 gcttcgctta tgatcgctac ccttgccacg cagcaagaca ttcctatcga tggaagtgac
     2101 gaagattttc aaacgttatg tatcgatatt ttgcatcatc cgcttaccaa atgtttggat
     2161 gaagaaaatg caaaaagcaa agccgaaaaa atcattttag aagaatgtaa gaatatagac
     2221 actgaagaaa agtaaaggag cttgaaaaca tgagtgaaac aaacgaaaca ggtatttata
     2281 tttttagcgc cattcaaacg gataaagacg aagaatttgg cgccgtggaa gtagaaggaa
     2341 caaaagctga aacatttttg attcgctaca aagacgcggc tatggtagca gctgaagtac
     2401 cgatgaaaat ttatcatcct aatcgccaaa atttattaat gcatcaaaac gcagtagcag
     2461 cgattatgga caagaacgat acggttattc caatcagctt tgggaatgta ttcaaatcaa
     2521 aagaagacgt aaaagttctt ttggaaaacc tttatccgca gtttgaaaag ctgtttccag
     2581 cgatcaaagg aaaaattgaa gtcggtttaa aagtaattgg gaaaaaagaa tggcttgaga
     2641 aaaaagtaaa cgaaaatcct gaacttgaga aagtatcagc atccgtaaaa ggaaaatcag
     2701 aagcagccgg ttattatgag cgtattcaac ttggaggaat ggctcaaaag atgtttactt
     2761 ccctgcaaaa agaagtcaag acagatgtgt tttctccgct tgaagaagca gcggaagcag
     2821 caaaagcaaa tgagccaacg ggcgaaacga tgcttttaaa cgcgtctttc ttaattaacc
     2881 gagaagatga agcgaagttt gatgaaaaag taaatgaagc gcatgaaaac tggaaagaca
     2941 aagccgattt tcattacagc ggtccttggc ctgcttataa ttttgtgaac attcgcctaa
     3001 aagtagaaga gaaataacgt gcttcacaaa ttagtaaccg cacccattaa ccttgtagtg
     3061 aaaatcggcg aaaaagtaca ggaagaagct gataaacagc tatatgacct tccgacgatt
     3121 cagcaaaagc tcattcagct tcaaatgatg tttgagcttg gtgaaattcc agaagaagcg
     3181 tttcaagaaa aagaagatga attgttaatg aggtacgaaa ttgcgaaacg cagagaaatt
     3241 gaacaatggg aagagctaac acaaaaaaga aatgaggaat cctagatggg agaattactg
     3301 tatttatacg gtttaattcc aacaaaagaa gcagcagcca tagagccgtt tccatcttat
     3361 aaggggtttg acggagaaca ttcactgtac ccaattgcgt ttgatcaggt gacggctgta
     3421 gtttctaagc tggatgctga cacctattca gaaaaagtga ttcaagaaaa aatggagcag
     3481 gatatgagct ggctgcagga aaaagcattt catcatcacg aaacggtagc cgctttgtac
     3541 gaagaattta cgatcattcc attaaaattt tgcaccattt ataaaggtga agaaagtctg
     3601 caggcagcta ttgagattaa caaagaaaag atagagaatt cactgacgct gcttcaagga
     3661 aatgaagagt ggaatgtgaa aatttactgt gatgatacag agcttaaaaa aggaatcagc
     3721 gaaacgaatg aaagcgtgaa agcgaaaaaa caagaaatta gtcacttatc accaggaaga
     3781 cagttttttg aaaagaaaaa aatagatcag ctgattgaaa aagaattaga gcttcacaaa
     3841 aacaaagtgt gtgaagagat acatgacaag ctaaaagaat tatcgcttta tgactctgtt
     3901 aaaaagaatt ggagcaaaga cgtaactggc gcagctgaac agatggcgtg gaacagcgtg
     3961 tttcttctcc cgtctctgca gattactaag ttcgtaaacg aaatagaaga gcttcagcaa
     4021 aggcttgaaa ataaaggctg gaagtttgaa gtgacgggac catggccgcc ctatcatttc
     4081 tcgagctttg cgtaaaagcg gttaagcgtg acagtccagg aggaattaat ggatgcaacc
     4141 ggtcagccaa gcaaatggac gaatccactt ggatcctgat caagctgaac aaggcttagc
     4201 gcagcttgtg atgacagtta ttgagctatt gaggcaaatc gttgaacgtc atgccatgag
     4261 gcgggtggag ggtggaacgt tgacggacga acaaattgaa aacttaggaa ttgcactaat
     4321 gaacttagaa gaaaaaatgg acgagttgaa agaggtgttc ggtctggatg cagaagattt
     4381 aaatattgat cttggaccgc taggcagcct gctttaagcg gtcagtagga ggaacagtat
     4441 ggcagtcgaa cataatatgc agtcaagtac gattgtagat gtgctcgaaa agattttgga
     4501 taaaggagtc gttatagcgg gggacatcac cgtaggaatt gcagatgtcg agctattaac
     4561 gataaagatc cgcttgattg tggcttcggt tgataaggca aaagaaatcg gcatggactg
     4621 gtgggaaaat gatccgtatc tcagttcaaa aggagccaat aacaaagcgc tcgaagaaga
     4681 aaataaaatg ctgcatgagc ggttaaaaac gcttgaagaa aaaatagaaa cgaaacgtta
     4741 aaaactgtac gctacttaaa aaatggaggg atttacaatg gcaactgaaa caaaattaga
     4801 taacacacag gcagaaaaca aggaaaataa aaatgcggaa aacggttcaa aagaaaagaa
     4861 cggttcaaaa gcaagcaaaa caacaagcag cgggccaatc aaacgagcgg tagcaggagg
     4921 catcatcggt gcaacgattg gatatgtatc gactcctgaa aatcgaaaaa gtctccttga
     4981 ccgcattgat acagacgaat taaaaagcaa agcatctgat ttaggaacaa aggtaaaaga
     5041 aaaatcaaaa agcagcgtgg ccagcctgaa aacatctgcg ggaagcttgt ttaaaaaaga
     5101 taaagataaa tcaaaagatg atgaagaaaa cgtaaattct tctagtagcg aaacagaaga
     5161 cgataacgtt caagagtacg acgagttaaa agaagaaaat caaactcttc aagatcgctt
     5221 atcacagctt gaagaaaaaa tgaacatgct tgttgagctt agcctcaata aaaatcaaga
     5281 cgaagaagcg gaagatacag attccgacga agaagagaac gatgagaacg atgaaaacga
     5341 tgaaaacgag caggacgatg aaaacgaaga agaaacatct aagccacgta aaaaggataa
     5401 aaaagaagct gaggaagaag aaagtgaaag tgacgaagac agcgaggaag aagaggaaga
     5461 ttctcgctca aacaaaaaaa ataaaaaagt aaaaacagaa gaagaagacg aagatgaaag
     5521 cgaagaagaa aaaaaggaag cgaaaccaaa aaagtcaaca gctaaaaaat caaaaaatac
     5581 aaaagcaaag aaaaacacgg acgaagaaga tgatgaagca acatctcttt ctagtgaaga
     5641 cgatacaaca gcctaagacg taaaggagga aagaaagaca tgagtacagg cccttctttt
     5701 tcaactaaag acaatacgct tgaatacttt gtgaaagctt ctaataaaca cggcttttca
     5761 cttgatattt cattaaatgt aaacggcgct gtgatttccg gtaccatgat ttcagcaaaa
     5821 gaatactttg attacttaag cgaaacgttt gaagaaggca gtgaagtggc tcaggcgcta
     5881 agcgaacaat tctctttagc aagcgaagcg agcgaatcaa acggagaagc agaagcccat
     5941 tttattcatt tgaaaaatac aaagatttac tgtggagaca gtaaatctac tccttctaaa
     6001 ggcaaaatct tttggagagg gaaaatagca gaagtagacg ggtttttctt aggaaagatt
     6061 tctgatgcaa aatcaacgag taaaaagagt tcataaaaaa cggcggggtg attgccccgc
     6121 cgttttttag tgatgtgatg agatgtgcag cttctttttt cgatacatga gcggagtcat
     6181 cttcatttct tttcgaaact gattaataaa atagcttgta ctattaaacc ctacttgata
     6241 ggcgacttcc gtcacatttg cttctgcttg ctgcagtccg gattcgcgaa cgcgtctcga
     6301 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     6361 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     6421 gaggaactat atccgactag tagcccgcct aatgagcggg ctttttttta attcccctat
     6481 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6541 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6601 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa
     6661 agtaaaagac gcagaggacc aattgggggc acgagtggga tacatagaac tggacttgaa
     6721 tagcggtaaa atccttgaga gttttcgccc tgaagagcgt tttccaatga tgagcacttt
     6781 caaagttctg ctatgtggag cagtattatc ccgtgtagat gcggggcaag agcaactcgg
     6841 acgacgaata cactattcgc agaatgactt ggttgaatac tccccagtga cagaaaagca
     6901 ccttacggac ggaatgacgg taagagaatt atgtagtgcc gccataacga tgagtgataa
     6961 cactgcggcg aacttacttc tgacaaccat cggtggaccg aaggaattaa ccgctttttt
     7021 gcacaatatg ggagaccatg taactcgcct tgaccgttgg gaaccagaac tgaatgaagc
     7081 cataccaaac gacgagcgag acaccacaat gcctgcggca atggcaacaa cattacgcaa
     7141 actattaact ggcgaactac ttactctggc ttcacggcaa caattaatag actggcttga
     7201 agcggataaa gttgcaggac cactactgcg ttcggcactt cctgctggct ggtttattgc
     7261 tgataaatct ggggcaggag agcgtggttc acggggtatc attgccgcac ttggaccaga
     7321 tggtaagcct tcccgtatcg tagttatcta cacgacgggt agtcaggcaa ctatggacga
     7381 acgaaataga cagattgctg aaataggggc ttcactgatt aagcattggt aaaccgatac
     7441 aattaaaggc tccttttgga gccttttttt ttggacggac cggtagaaaa gatcaaagga
     7501 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7561 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7621 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7681 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7741 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7801 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7861 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7921 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7981 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8041 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8101 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8161 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8221 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8281 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
     8341 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     8401 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8461 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8521 tcagaggttt tcaccgtcat cac
//
