LOCUS       sequence-445915-        8579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaK
FEATURES             Location/Qualifiers
     source          1..8579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1432..1451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1433..1451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1688..1879
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1873..1895)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1981..2120
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2036..2055
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2135..2152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2306..2894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2386..2405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2925..2964)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        complement(3829..3920)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(3987..4034)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4030..4048
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(7606..7623)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
ORIGIN
        1 gtatcatata cgccggcata ctcagctgga ttgcttgtga agatgacgcg gaaatcggga
       61 tgcacgcgca caaaaggatc ggtcattttt actccataca gcggcagcac gccttcttct
      121 aatatcgata gaaagatatt attcgtcgca ggcttagaac gagtaaattc gtcgtaaatc
      181 agcgtatagc cattttttac agcttcaagc aatcggccat cctgccagtt ttcactcacc
      241 tgttcatctt ttttatagac agaacgaacg tactggtcga ttactttttt gctcgtgtat
      301 cccgtaaaat cgccaattaa atctttgttg ttgagctcgt gattcccgtg catcagcatt
      361 acaggacgct ttctcttttt agcaagcgct cgcgctaaag aggttttgcc tccgccggca
      421 ggacctgtaa aatgaatgga atagccggat tttaaatagc tcagcgctct tgaaagaacc
      481 tcttttgtct cgtcatcttg tataaaagct ccactgcctt ttttcctttt gtctgttaag
      541 acggtcattt ttacgttcct cctgccatct tatcgtttat gcttcaatgc caatggcgct
      601 tctatatctg acgtcgagtc gtttgaatga aatgacttct ttttctttat ttacaaagca
      661 ctcgtacgtt ccgagcattt catctttggc gtattttttc atatattctc gttcttcaat
      721 gacttcaaca ataactctcc aaccttcatc ttcagtagct tccaccgagg taattttatg
      781 aggaggagct acgtgttcac cgaaaaagtc gttcacggct tgcataattt ttttaatttc
      841 catttttagt agcctccaat agttagatct aaaagctgga accaccctca aaaaaggaag
      901 gttccatacc acttcatatt aaatactaaa acgcggctgc ccttcacttg aatttgaacg
      961 ttcaggaaga ccgttttctt ctacgtcgtc acgaagaagc cctactgctt ctgcatagcg
     1021 taaccatgta tcaacactgg caataaccac tcgcgcttca atcgttaaaa tttcaattcc
     1081 tacaacagaa actcttgcaa aagcatcaat aacaattcct ttatctaaaa tacggtcaat
     1141 gacttctgct aaacttgaac tattagtact tttttgaata gacatatctc atttctcctt
     1201 tcgcttatga tcccattgtt tttaaatctg tagatgattt aatatcatta gaacttttaa
     1261 tcttagtaga gcttttaata tccgttgctt ttttaatctg atttacgcct tttattttcc
     1321 gcttattttt tgacttctct ttatcgtctg aagaagaaac tttttcttga aagttttctc
     1381 cagcttcttt cacatcgcta agctatctct agagggaaac cgttgtggtc tccctatagt
     1441 gagtcgtatt aatttcgcgg gatcgatgca tggtgccaac acctacatct gtattaacga
     1501 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
     1561 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
     1621 gcatcctctc tcgtttcatc ggtatcatta ccccatgaac agaaatcccc cttacacgga
     1681 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     1741 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
     1801 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
     1861 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     1921 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     1981 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     2041 tcagagcaga ttgtactgag agtgcaccat tgcggtgtga aataccgcac agatgcgtaa
     2101 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2161 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2881 aaaaaggatc tcaagaagat cctttgatct tttctaccgg tccgtccaaa aaaaaaggct
     2941 ccaaaaggag cctttaattg tatcggttta ccaatgctta atcagtgaag cccctatttc
     3001 agcaatctgt ctatttcgtt cgtccatagt tgcctgacta cccgtcgtgt agataactac
     3061 gatacgggaa ggcttaccat ctggtccaag tgcggcaatg ataccccgtg aaccacgctc
     3121 tcctgcccca gatttatcag caataaacca gccagcagga agtgccgaac gcagtagtgg
     3181 tcctgcaact ttatccgctt caagccagtc tattaattgt tgccgtgaag ccagagtaag
     3241 tagttcgcca gttaatagtt tgcgtaatgt tgttgccatt gccgcaggca ttgtggtgtc
     3301 tcgctcgtcg tttggtatgg cttcattcag ttctggttcc caacggtcaa ggcgagttac
     3361 atggtctccc atattgtgca aaaaagcggt taattccttc ggtccaccga tggttgtcag
     3421 aagtaagttc gccgcagtgt tatcactcat cgttatggcg gcactacata attctcttac
     3481 cgtcattccg tccgtaaggt gcttttctgt cactggggag tattcaacca agtcattctg
     3541 cgaatagtgt attcgtcgtc cgagttgctc ttgccccgca tctacacggg ataatactgc
     3601 tccacatagc agaactttga aagtgctcat cattggaaaa cgctcttcag ggcgaaaact
     3661 ctcaaggatt ttaccgctat tcaagtccag ttctatgtat cccactcgtg cccccaattg
     3721 gtcctctgcg tcttttactt tcacgagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3901 tatttagaaa aataaacaaa taggggaatt aaaaaaaagc ccgctcatta ggcgggctac
     3961 tagtcggata tagttcctcc tttcagcaaa aaacccctca agacccgttt agaggcccca
     4021 aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc ctttcgggct
     4081 ttgttagcag ccggatctct cgagacgcgt tcgcgaatcc ggactgcagc aagcagaagc
     4141 aaatgtgacg gaagtcgcct atcaagtagg gtttaatagt acaagctatt ttattaatca
     4201 gtttcgaaaa gaaatgaaga tgactccgct catgtatcga aaaaagaagc tgcacatctc
     4261 atcacatcac taaaaaacgg cggggcaatc accccgccgt tttttatgaa ctctttttac
     4321 tcgttgattt tgcatcagaa atctttccta agaaaaaccc gtctacttct gctattttcc
     4381 ctctccaaaa gattttgcct ttagaaggag tagatttact gtctccacag taaatctttg
     4441 tatttttcaa atgaataaaa tgggcttctg cttctccgtt tgattcgctc gcttcgcttg
     4501 ctaaagagaa ttgttcgctt agcgcctgag ccacttcact gccttcttca aacgtttcgc
     4561 ttaagtaatc aaagtattct tttgctgaaa tcatggtacc ggaaatcaca gcgccgttta
     4621 catttaatga aatatcaagt gaaaagccgt gtttattaga agctttcaca aagtattcaa
     4681 gcgtattgtc tttagttgaa aaagaagggc ctgtactcat gtctttcttt cctcctttac
     4741 gtcttaggct gttgtatcgt cttcactaga aagagatgtt gcttcatcat cttcttcgtc
     4801 cgtgtttttc tttgcttttg tattttttga ttttttagct gttgactttt ttggtttcgc
     4861 ttcctttttt tcttcttcgc tttcatcttc gtcttcttct tctgttttta cttttttatt
     4921 ttttttgttt gagcgagaat cttcctcttc ttcctcgctg tcttcgtcac tttcactttc
     4981 ttcttcctca gcttcttttt tatccttttt acgtggctta gatgtttctt cttcgttttc
     5041 atcgtcctgc tcgttttcat cgttttcatc gttctcatcg ttctcttctt cgtcggaatc
     5101 tgtatcttcc gcttcttcgt cttgattttt attgaggcta agctcaacaa gcatgttcat
     5161 tttttcttca agctgtgata agcgatcttg aagagtttga ttttcttctt ttaactcgtc
     5221 gtactcttga acgttatcgt cttctgtttc gctactagaa gaatttacgt tttcttcatc
     5281 atcttttgat ttatctttat cttttttaaa caagcttccc gcagatgttt tcaggctggc
     5341 cacgctgctt tttgattttt cttttacctt tgttcctaaa tcagatgctt tgctttttaa
     5401 ttcgtctgta tcaatgcggt caaggagact ttttcgattt tcaggagtcg atacatatcc
     5461 aatcgttgca ccgatgatgc ctcctgctac cgctcgtttg attggcccgc tgcttgttgt
     5521 tttgcttgct tttgaaccgt tcttttcttt tgaaccgttt tccgcatttt tattttcctt
     5581 gttttctgcc tgtgtgttat ctaattttgt ttcagttgcc attgtaaatc cctccatttt
     5641 ttaagtagcg tacagttttt aacgtttcgt ttctattttt tcttcaagcg tttttaaccg
     5701 ctcatgcagc attttatttt cttcttcgag cgctttgtta ttggctcctt ttgaactgag
     5761 atacggatca ttttcccacc agtccatgcc gatttctttt gccttatcaa ccgaagccac
     5821 aatcaagcgg atctttatcg ttaatagctc gacatctgca attcctacgg tgatgtcccc
     5881 cgctataacg actcctttat ccaaaatctt ttcgagcaca tctacaatcg tacttgactg
     5941 catattatgt tcgactgcca tactgttcct cctactgacc gcctgtcacg cttaaccgct
     6001 cggaattcga gaaagcatac gactgctcgc aagggtcacg aacctagagg gttcgtccac
     6061 ttactaggct ggcccgtagc gtccatcagc tcttcttttt gtttatcaaa ttgttcagaa
     6121 gtgattggtt tgtgatttcc ttcttttgct tgcacaagcg tttcaaccga agaaataagc
     6181 acccgcaaat ccaaatacac taaatcgacg ccagctatgg aaatgattaa gtctccttta
     6241 atggcgactc ctttatctaa aatgacatct aaaatatcaa taagagcaat atctttattc
     6301 tccatggatt gtttaagaga cataatgtta attcctcact ttacgcaaag ctcgagaaat
     6361 gatagggcgg ccatggtccc gtcacttcaa acttccagcc tttattttca agcctttgct
     6421 gaagctcttc tatttcgttt acgaacttag taatctgcag agacgggaga agaaacacgc
     6481 tgttccacgc catctgttca gctgcgccag ttacgtcttt gctccaattc tttttaacag
     6541 agtcataaag cgataattct tttagcttgt catgtatctc ttcacacact ttgtttttgt
     6601 gaagctctaa ttctttttca atcagctgat ctattttttt cttttcaaaa aactgtcttc
     6661 ctggtgataa gtgactaatt tcttgttttt tcgctttcac gctttcattc gtttcgctga
     6721 ttcctttttt aagctctgta tcatcacagt aaattttcac attccactct tcatttcctt
     6781 gaagcagcgt cagtgaattc tctatctttt ctttgttaat ctcaatagct gcctgcagac
     6841 tttcttcacc tttataaatg gtgcaaaatt ttaatggaat gatcgtaaat tcttcgtaca
     6901 aagcggctac cgtttcgtga tgatgaaatg ctttttcctg cagccagctc atatcctgct
     6961 ccattttttc ttgaatcact ttttctgaat aggtgtcagc atccagctta gaaactacag
     7021 ccgtcacctg atcaaacgca attgggtaca gtgaatgttc tccgtcaaac cccttataag
     7081 atggaaacgg ctctatggct gctgcttctt ttgttggaat taaaccgtat aaatacagta
     7141 attctcccat ctaggattcc tcatttcttt tttgtgttag ctcttcccat tgttcaattt
     7201 ctctgcgttt cgcaatttcg tacctcatta acaattcatc ttctttttct tgaaacgctt
     7261 cttctggaat ttcaccaagc tcaaacatca tttgaagctg aatgagcttt tgctgaatcg
     7321 tcggaaggtc atatagctgt ttatcagctt cttcctgtac tttttcgccg attttcacta
     7381 caaggttaat gggtgcggtt actaatttgt gaagcacgtt atttctcttc tacttttagg
     7441 cgaatgttca caaaattata agcaggccaa ggaccgctgt aatgaaaatc ggctttgtct
     7501 ttccagtttt catgcgcttc atttactttt tcatcaaact tcgcttcatc ttctcggtta
     7561 attaagaaag acgcgtttaa aagcatcgtt tcgcccgttg gctcatttgc ttttgctgct
     7621 tccgctgctt cttcaagcgg agaaaacaca tctgtcttga cttctttttg cagggaagta
     7681 aacatctttt gagccattcc tccaagttga atacgctcat aataaccggc tgcttctgat
     7741 tttcctttta cggatgctga tactttctca agttcaggat tttcgtttac ttttttctca
     7801 agccattctt ttttcccaat tacttttaaa ccgacttcaa tttttccttt gatcgctgga
     7861 aacagctttt caaactgcgg ataaaggttt tccaaaagaa cttttacgtc ttcttttgat
     7921 ttgaatacat tcccaaagct gattggaata accgtatcgt tcttgtccat aatcgctgct
     7981 actgcgtttt gatgcattaa taaattttgg cgattaggat gataaatttt catcggtact
     8041 tcagctgcta ccatagccgc gtctttgtag cgaatcaaaa atgtttcagc ttttgttcct
     8101 tctacttcca cggcgccaaa ttcttcgtct ttatccgttt gaatggcgct aaaaatataa
     8161 atacctgttt cgtttgtttc actcatgttt tcaagctcct ttacttttct tcagtgtcta
     8221 tattcttaca ttcttctaaa atgatttttt cggctttgct ttttgcattt tcttcatcca
     8281 aacatttggt aagcggatga tgcaaaatat cgatacataa cgtttgaaaa tcttcgtcac
     8341 ttccatcgat aggaatgtct tgctgcgtgg caagggtagc gatcataagc gaagcccgca
     8401 ggctaagtcc gctgctgttt tcgtctccag agcggtttcg cacgtttgct acgagcgtta
     8461 caattgtgcg cgcttcatct tcttctacgt ccgttttctc cgttaaaatc gctgtctctc
     8521 tgtcgatgtc tttataatca ataaacatgg taattaacct gtcgagaagc gcatcttgc
//
