LOCUS       sequence-445972-        7238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN and GvpU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNU
FEATURES             Location/Qualifiers
     source          1..7238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2324..2341
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5496..5514)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5510..5557
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5624..5715
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6580..6619
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6650..7238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7139..7158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcgtaaagga gcttgaaaac atgagtgaaa caaacgaaac aggtatttat atttttagcg
     1801 ccattcaaac ggataaagac gaagaatttg gcgccgtgga agtagaagga acaaaagctg
     1861 aaacattttt gattcgctac aaagacgcgg ctatggtagc agctgaagta ccgatgaaaa
     1921 tttatcatcc taatcgccaa aatttattaa tgcatcaaaa cgcagtagca gcgattatgg
     1981 acaagaacga tacggttatt ccaatcagct ttgggaatgt attcaaatca aaagaagacg
     2041 taaaagttct tttggaaaac ctttatccgc agtttgaaaa gctgtttcca gcgatcaaag
     2101 gaaaaattga agtcggttta aaagtaattg ggaaaaaaga atggcttgag aaaaaagtaa
     2161 acgaaaatcc tgaacttgag aaagtatcag catccgtaaa aggaaaatca gaagcagccg
     2221 gttattatga gcgtattcaa cttggaggaa tggctcaaaa gatgtttact tccctgcaaa
     2281 aagaagtcaa gacagatgtg ttttctccgc ttgaagaagc agcggaagca gcaaaagcaa
     2341 atgagccaac gggcgaaacg atgcttttaa acgcgtcttt cttaattaac cgagaagatg
     2401 aagcgaagtt tgatgaaaaa gtaaatgaag cgcatgaaaa ctggaaagac aaagccgatt
     2461 ttcattacag cggtccttgg cctgcttata attttgtgaa cattcgccta aaagtagaag
     2521 agaaataacg tgcttcacaa attagtaacc gcacccatta accttgtagt gaaaatcggc
     2581 gaaaaagtac aggaagaagc tgataaacag ctatatgacc ttccgacgat tcagcaaaag
     2641 ctcattcagc ttcaaatgat gtttgagctt ggtgaaattc cagaagaagc gtttcaagaa
     2701 aaagaagatg aattgttaat gaggtacgaa attgcgaaac gcagagaaat tgaacaatgg
     2761 gaagagctaa cacaaaaaag aaatgaggaa tcctagatgg gagaattact gtatttatac
     2821 ggtttaattc caacaaaaga agcagcagcc atagagccgt ttccatctta taaggggttt
     2881 gacggagaac attcactgta cccaattgcg tttgatcagg tgacggctgt agtttctaag
     2941 ctggatgctg acacctattc agaaaaagtg attcaagaaa aaatggagca ggatatgagc
     3001 tggctgcagg aaaaagcatt tcatcatcac gaaacggtag ccgctttgta cgaagaattt
     3061 acgatcattc cattaaaatt ttgcaccatt tataaaggtg aagaaagtct gcaggcagct
     3121 attgagatta acaaagaaaa gatagagaat tcactgacgc tgcttcaagg aaatgaagag
     3181 tggaatgtga aaatttactg tgatgataca gagcttaaaa aaggaatcag cgaaacgaat
     3241 gaaagcgtga aagcgaaaaa acaagaaatt agtcacttat caccaggaag acagtttttt
     3301 gaaaagaaaa aaatagatca gctgattgaa aaagaattag agcttcacaa aaacaaagtg
     3361 tgtgaagaga tacatgacaa gctaaaagaa ttatcgcttt atgactctgt taaaaagaat
     3421 tggagcaaag acgtaactgg cgcagctgaa cagatggcgt ggaacagcgt gtttcttctc
     3481 ccgtctctgc agattactaa gttcgtaaac gaaatagaag agcttcagca aaggcttgaa
     3541 aataaaggct ggaagtttga agtgacggga ccatggccgc cctatcattt ctcgagcttt
     3601 gcgtaaagtg aggaattaac attatgtctc ttaaacaatc catggagaat aaagatattg
     3661 ctcttattga tattttagat gtcattttag ataaaggagt cgccattaaa ggagacttaa
     3721 tcatttccat agctggcgtc gatttagtgt atttggattt gcgggtgctt atttcttcgg
     3781 ttgaaacgct tgtgcaagca aaagaaggaa atcacaaacc aatcacttct gaacaatttg
     3841 ataaacaaaa ggaggaatta atggatgcaa ccggtcagcc aagcaaatgg acgaatccac
     3901 ttggatcctg atcaagctga acaaggctta gcgcagcttg tgatgacagt tattgagcta
     3961 ttgaggcaaa tcgttgaacg tcatgccatg aggcgggtgg agggtggaac gttgacggac
     4021 gaacaaattg aaaacttagg aattgcacta atgaacttag aagaaaaaat ggacgagttg
     4081 aaagaggtgt tcggtctgga tgcagaagat ttaaatattg atcttggacc gctaggcagc
     4141 ctgctttaag cggtcagtag gaggaacagt atggcagtcg aacataatat gcagtcaagt
     4201 acgattgtag atgtgctcga aaagattttg gataaaggag tcgttatagc gggggacatc
     4261 accgtaggaa ttgcagatgt cgagctatta acgataaaga tccgcttgat tgtggcttcg
     4321 gttgataagg caaaagaaat cggcatggac tggtgggaaa atgatccgta tctcagttca
     4381 aaaggagcca ataacaaagc gctcgaagaa gaaaataaaa tgctgcatga gcggttaaaa
     4441 acgcttgaag aaaaaataga aacgaaacgt taaaaactgt acgctactta aaaaatggag
     4501 ggatttacaa tggcaactga aacaaaatta gataacacac aggcagaaaa caaggaaaat
     4561 aaaaatgcgg aaaacggttc aaaagaaaag aacggttcaa aagcaagcaa aacaacaagc
     4621 agcgggccaa tcaaacgagc ggtagcagga ggcatcatcg gtgcaacgat tggatatgta
     4681 tcgactcctg aaaatcgaaa aagtctcctt gaccgcattg atacagacga attaaaaagc
     4741 aaagcatctg atttaggaac aaaggtaaaa gaaaaatcaa aaagcagcgt ggccagcctg
     4801 aaaacatctg cgggaagctt gtttaaaaaa gataaagata aatcaaaaga tgatgaagaa
     4861 aacgtaaatt cttctagtag cgaaacagaa gacgataacg ttcaagagta cgacgagtta
     4921 aaagaagaaa atcaaactct tcaagatcgc ttatcacagc ttgaagaaaa aatgaacatg
     4981 cttgttgagc ttagcctcaa taaaaatcaa gacgaagaag cggaagatac agattccgac
     5041 gaagaagaga acgatgagaa cgatgaaaac gatgaaaacg agcaggacga tgaaaacgaa
     5101 gaagaaacat ctaagccacg taaaaaggat aaaaaagaag ctgaggaaga agaaagtgaa
     5161 agtgacgaag acagcgagga agaagaggaa gattctcgct caaacaaaaa aaataaaaaa
     5221 gtaaaaacag aagaagaaga cgaagatgaa agcgaagaag aaaaaaagga agcgaaacca
     5281 aaaaagtcaa cagctaaaaa atcaaaaaat acaaaagcaa agaaaaacac ggacgaagaa
     5341 gatgatgaag caacatctct ttctagtgaa gacgatacaa cagcctaaga cgtcgtcaca
     5401 tttgcttctg cttgctgcag tccggattcg cgaacgcgtc tcgagagatc cggctgctaa
     5461 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     5521 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccga
     5581 ctagtagccc gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa
     5641 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5701 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5761 gcattttgcc ttcctgtttt tgctcaccca gaaacgctcg tgaaagtaaa agacgcagag
     5821 gaccaattgg gggcacgagt gggatacata gaactggact tgaatagcgg taaaatcctt
     5881 gagagttttc gccctgaaga gcgttttcca atgatgagca ctttcaaagt tctgctatgt
     5941 ggagcagtat tatcccgtgt agatgcgggg caagagcaac tcggacgacg aatacactat
     6001 tcgcagaatg acttggttga atactcccca gtgacagaaa agcaccttac ggacggaatg
     6061 acggtaagag aattatgtag tgccgccata acgatgagtg ataacactgc ggcgaactta
     6121 cttctgacaa ccatcggtgg accgaaggaa ttaaccgctt ttttgcacaa tatgggagac
     6181 catgtaactc gccttgaccg ttgggaacca gaactgaatg aagccatacc aaacgacgag
     6241 cgagacacca caatgcctgc ggcaatggca acaacattac gcaaactatt aactggcgaa
     6301 ctacttactc tggcttcacg gcaacaatta atagactggc ttgaagcgga taaagttgca
     6361 ggaccactac tgcgttcggc acttcctgct ggctggttta ttgctgataa atctggggca
     6421 ggagagcgtg gttcacgggg tatcattgcc gcacttggac cagatggtaa gccttcccgt
     6481 atcgtagtta tctacacgac gggtagtcag gcaactatgg acgaacgaaa tagacagatt
     6541 gctgaaatag gggcttcact gattaagcat tggtaaaccg atacaattaa aggctccttt
     6601 tggagccttt ttttttggac ggaccggtag aaaagatcaa aggatcttct tgagatcctt
     6661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6781 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7021 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaa
//
