LOCUS       sequence-44598-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Wadia JS, Stan RV, Dowdy SF
  TITLE     Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion 
            proteins after lipid raft macropinocytosis.
  JOURNAL   Nat Med. 2004 Mar;10(3):310-5. Epub 2004 Feb 8.
  PUBMED    14770178
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2004-03"; volume: "10(3)"; pages: "310-5. Epub 2004 Feb 8"
COMMENT     Sequence Label: pTAT2.1
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             173..190
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(243..261)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      257..304
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      341..796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(428..447)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     638..659
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgaggaag aagcggagac agcgacgaag
      121 aggctcggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca
      181 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      241 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      301 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      361 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      421 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      481 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      541 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      601 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      661 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      721 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      781 ttaacgctta caatttaggt ggcacttttc gg
//
