LOCUS       sequence-446031-        8293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpG and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaGS
FEATURES             Location/Qualifiers
     source          1..8293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6551..6569)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6565..6612
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6679..6770
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7635..7674
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7705..8293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8194..8213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttttccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgaa atgaggaatc
     3481 ctagatggga gaattactgt atttatacgg tttaattcca acaaaagaag cagcagccat
     3541 agagccgttt ccatcttata aggggtttga cggagaacat tcactgtacc caattgcgtt
     3601 tgatcaggtg acggctgtag tttctaagct ggatgctgac acctattcag aaaaagtgat
     3661 tcaagaaaaa atggagcagg atatgagctg gctgcaggaa aaagcatttc atcatcacga
     3721 aacggtagcc gctttgtacg aagaatttac gatcattcca ttaaaatttt gcaccattta
     3781 taaaggtgaa gaaagtctgc aggcagctat tgagattaac aaagaaaaga tagagaattc
     3841 actgacgctg cttcaaggaa atgaagagtg gaatgtgaaa atttactgtg atgatacaga
     3901 gcttaaaaaa ggaatcagcg aaacgaatga aagcgtgaaa gcgaaaaaac aagaaattag
     3961 tcacttatca ccaggaagac agttttttga aaagaaaaaa atagatcagc tgattgaaaa
     4021 agaattagag cttcacaaaa acaaagtgtg tgaagagata catgacaagc taaaagaatt
     4081 atcgctttat gactctgtta aaaagaattg gagcaaagac gtaactggcg cagctgaaca
     4141 gatggcgtgg aacagcgtgt ttcttctccc gtctctgcag attactaagt tcgtaaacga
     4201 aatagaagag cttcagcaaa ggcttgaaaa taaaggctgg aagtttgaag tgacgggacc
     4261 atggccgccc tatcatttct cgagctttgc gtaaaagcgg ttaagcgtga cagtccagga
     4321 ggaattaatg gatgcaaccg gtcagccaag caaatggacg aatccacttg gatcctgatc
     4381 aagctgaaca aggcttagcg cagcttgtga tgacagttat tgagctattg aggcaaatcg
     4441 ttgaacgtca tgccatgagg cgggtggagg gtggaacgtt gacggacgaa caaattgaaa
     4501 acttaggaat tgcactaatg aacttagaag aaaaaatgga cgagttgaaa gaggtgttcg
     4561 gtctggatgc agaagattta aatattgatc ttggaccgct aggcagcctg ctttaagcgg
     4621 tcagtaggag gaacagtatg gcagtcgaac ataatatgca gtcaagtacg attgtagatg
     4681 tgctcgaaaa gattttggat aaaggagtcg ttatagcggg ggacatcacc gtaggaattg
     4741 cagatgtcga gctattaacg ataaagatcc gcttgattgt ggcttcggtt gataaggcaa
     4801 aagaaatcgg catggactgg tgggaaaatg atccgtatct cagttcaaaa ggagccaata
     4861 acaaagcgct cgaagaagaa aataaaatgc tgcatgagcg gttaaaaacg cttgaagaaa
     4921 aaatagaaac gaaacgttaa aaactgtacg ctacttaaaa aatggaggga tttacaatgg
     4981 caactgaaac aaaattagat aacacacagg cagaaaacaa ggaaaataaa aatgcggaaa
     5041 acggttcaaa agaaaagaac ggttcaaaag caagcaaaac aacaagcagc gggccaatca
     5101 aacgagcggt agcaggaggc atcatcggtg caacgattgg atatgtatcg actcctgaaa
     5161 atcgaaaaag tctccttgac cgcattgata cagacgaatt aaaaagcaaa gcatctgatt
     5221 taggaacaaa ggtaaaagaa aaatcaaaaa gcagcgtggc cagcctgaaa acatctgcgg
     5281 gaagcttgtt taaaaaagat aaagataaat caaaagatga tgaagaaaac gtaaattctt
     5341 ctagtagcga aacagaagac gataacgttc aagagtacga cgagttaaaa gaagaaaatc
     5401 aaactcttca agatcgctta tcacagcttg aagaaaaaat gaacatgctt gttgagctta
     5461 gcctcaataa aaatcaagac gaagaagcgg aagatacaga ttccgacgaa gaagagaacg
     5521 atgagaacga tgaaaacgat gaaaacgagc aggacgatga aaacgaagaa gaaacatcta
     5581 agccacgtaa aaaggataaa aaagaagctg aggaagaaga aagtgaaagt gacgaagaca
     5641 gcgaggaaga agaggaagat tctcgctcaa acaaaaaaaa taaaaaagta aaaacagaag
     5701 aagaagacga agatgaaagc gaagaagaaa aaaaggaagc gaaaccaaaa aagtcaacag
     5761 ctaaaaaatc aaaaaataca aaagcaaaga aaaacacgga cgaagaagat gatgaagcaa
     5821 catctctttc tagtgaagac gatacaacag cctaagacgt aaaggaggaa agaaagacat
     5881 gagtacaggc ccttcttttt caactaaaga caatacgctt gaatactttg tgaaagcttc
     5941 taataaacac ggcttttcac ttgatatttc attaaatgta aacggcgctg tgatttccgg
     6001 taccatgatt tcagcaaaag aatactttga ttacttaagc gaaacgtttg aagaaggcag
     6061 tgaagtggct caggcgctaa gcgaacaatt ctctttagca agcgaagcga gcgaatcaaa
     6121 cggagaagca gaagcccatt ttattcattt gaaaaataca aagatttact gtggagacag
     6181 taaatctact ccttctaaag gcaaaatctt ttggagaggg aaaatagcag aagtagacgg
     6241 gtttttctta ggaaagattt ctgatgcaaa atcaacgagt aaaaagagtt cataaaaaac
     6301 ggcggggtga ttgccccgcc gttttttagt gatgtgatga gatgtgcagc ttcttttttc
     6361 gatacatgag cggagtcatc ttcatttctt ttcgaaactg attaataaaa tagcttgtac
     6421 tattaaaccc tacttgatag gcgacttccg tcacatttgc ttctgcttgc tgcagtccgg
     6481 attcgcgaac gcgtctcgag agatccggct gctaacaaag cccgaaagga agctgagttg
     6541 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     6601 aggggttttt tgctgaaagg aggaactata tccgactagt agcccgccta atgagcgggc
     6661 ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6721 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6781 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6841 acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca cgagtgggat
     6901 acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct gaagagcgtt
     6961 ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc cgtgtagatg
     7021 cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg gttgaatact
     7081 ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta tgtagtgccg
     7141 ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc ggtggaccga
     7201 aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt gaccgttggg
     7261 aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg cctgcggcaa
     7321 tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct tcacggcaac
     7381 aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt tcggcacttc
     7441 ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca cggggtatca
     7501 ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac acgacgggta
     7561 gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct tcactgatta
     7621 agcattggta aaccgataca attaaaggct ccttttggag cctttttttt tggacggacc
     7681 ggtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7741 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7801 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7861 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7921 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7981 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8041 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8101 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8161 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8221 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8281 gagcctatgg aaa
//
