LOCUS       sequence-446033-        7620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB and GvpL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBL
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2927..2944
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5877..5895)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5891..5938
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6005..6096
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7032..7620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7521..7540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatt agatctaact attggaggct actaaaaatg gaaattaaaa
     1141 aaattatgca agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta
     1201 cctcggtgga agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac
     1261 gagaatatat gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa
     1321 ataaagaaaa agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg
     1381 gcattgaagc ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac
     1441 aaaaggaaaa aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca
     1501 agagcgctga gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga
     1561 ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg
     1621 cacgggaatc acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc
     1681 aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa
     1741 aactggcagg atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac
     1801 gaatttactc gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc
     1861 gtgctgccgc tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc
     1921 cgcgtcatct tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg
     1981 cttctcgaca ggttaattac catgtttatt gattataaag acatcgacag agagacagcg
     2041 attttaacgg agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta
     2101 gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg
     2161 cttatgatcg ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat
     2221 tttcaaacgt tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa
     2281 aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa
     2341 gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga aacaggtatt tatattttta
     2401 gcgccattca aacggataaa gacgaagaat ttggcgccgt ggaagtagaa ggaacaaaag
     2461 ctgaaacatt tttgattcgc tacaaagacg cggctatggt agcagctgaa gtaccgatga
     2521 aaatttatca tcctaatcgc caaaatttat taatgcatca aaacgcagta gcagcgatta
     2581 tggacaagaa cgatacggtt attccaatca gctttgggaa tgtattcaaa tcaaaagaag
     2641 acgtaaaagt tcttttggaa aacctttatc cgcagtttga aaagctgttt ccagcgatca
     2701 aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa agaatggctt gagaaaaaag
     2761 taaacgaaaa tcctgaactt gagaaagtat cagcatccgt aaaaggaaaa tcagaagcag
     2821 ccggttatta tgagcgtatt caacttggag gaatggctca aaagatgttt acttccctgc
     2881 aaaaagaagt caagacagat gtgttttctc cgcttgaaga agcagcggaa gcagcaaaag
     2941 caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc tttcttaatt aaccgagaag
     3001 atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga aaactggaaa gacaaagccg
     3061 attttcatta cagcggtcct tggcctgctt ataattttgt gaacattcgc ctaaaagtag
     3121 aagagaaata acgtgcttca caaattagta accgcaccca ttaaccttgt agtgaaaatc
     3181 ggcgaaaaag tacaggaaga agctgataaa cagctatatg accttccgac gattcagcaa
     3241 aagctcattc agcttcaaat gatgtttgag cttggtgaaa ttccagaaga agcgtttcaa
     3301 gaaaaagaag atgaattgtt aatgaggtac gaaattgcga aacgcagaga aattgaacaa
     3361 tgggaagagc taccacaaaa aagaaatgag gaatcctaga gtgaggaatt aacattatgt
     3421 ctcttaaaca atccatggag aataaagata ttgctcttat tgatatttta gatgtcattt
     3481 tagataaagg agtcgccatt aaaggagact taatcatttc catagctggc gtcgatttag
     3541 tgtatttgga tttgcgggtg cttatttctt cggttgaaac gcttgtgcaa gcaaaagaag
     3601 gaaatcacaa accaatcact tctgaacaat ttgataaaca aaaggaggaa ttaatggatg
     3661 caaccggtca gccaagcaaa tggacgaatc cacttggatc ctgatcaagc tgaacaaggc
     3721 ttagcgcagc ttgtgatgac agttattgag ctattgaggc aaatcgttga acgtcatgcc
     3781 atgaggcggg tggagggtgg aacgttgacg gacgaacaaa ttgaaaactt aggaattgca
     3841 ctaatgaact tagaagaaaa aatggacgag ttgaaagagg tgttcggtct ggatgcagaa
     3901 gatttaaata ttgatcttgg accgctaggc agcctgcttt aagcggtcag taggaggaac
     3961 agtatggcag tcgaacataa tatgcagtca agtacgattg tagatgtgct cgaaaagatt
     4021 ttggataaag gagtcgttat agcgggggac atcaccgtag gaattgcaga tgtcgagcta
     4081 ttaacgataa agatccgctt gattgtggct tcggttgata aggcaaaaga aatcggcatg
     4141 gactggtggg aaaatgatcc gtatctcagt tcaaaaggag ccaataacaa agcgctcgaa
     4201 gaagaaaata aaatgctgca tgagcggtta aaaacgcttg aagaaaaaat agaaacgaaa
     4261 cgttaaaaac tgtacgctac ttaaaaaatg gagggattta caatggcaac tgaaacaaaa
     4321 ttagataaca cacaggcaga aaacaaggaa aataaaaatg cggaaaacgg ttcaaaagaa
     4381 aagaacggtt caaaagcaag caaaacaaca agcagcgggc caatcaaacg agcggtagca
     4441 ggaggcatca tcggtgcaac gattggatat gtatcgactc ctgaaaatcg aaaaagtctc
     4501 cttgaccgca ttgatacaga cgaattaaaa agcaaagcat ctgatttagg aacaaaggta
     4561 aaagaaaaat caaaaagcag cgtggccagc ctgaaaacat ctgcgggaag cttgtttaaa
     4621 aaagataaag ataaatcaaa agatgatgaa gaaaacgtaa attcttctag tagcgaaaca
     4681 gaagacgata acgttcaaga gtacgacgag ttaaaagaag aaaatcaaac tcttcaagat
     4741 cgcttatcac agcttgaaga aaaaatgaac atgcttgttg agcttagcct caataaaaat
     4801 caagacgaag aagcggaaga tacagattcc gacgaagaag agaacgatga gaacgatgaa
     4861 aacgatgaaa acgagcagga cgatgaaaac gaagaagaaa catctaagcc acgtaaaaag
     4921 gataaaaaag aagctgagga agaagaaagt gaaagtgacg aagacagcga ggaagaagag
     4981 gaagattctc gctcaaacaa aaaaaataaa aaagtaaaaa cagaagaaga agacgaagat
     5041 gaaagcgaag aagaaaaaaa ggaagcgaaa ccaaaaaagt caacagctaa aaaatcaaaa
     5101 aatacaaaag caaagaaaaa cacggacgaa gaagatgatg aagcaacatc tctttctagt
     5161 gaagacgata caacagccta agacgtaaag gaggaaagaa agacatgagt acaggccctt
     5221 ctttttcaac taaagacaat acgcttgaat actttgtgaa agcttctaat aaacacggct
     5281 tttcacttga tatttcatta aatgtaaacg gcgctgtgat ttccggtacc atgatttcag
     5341 caaaagaata ctttgattac ttaagcgaaa cgtttgaaga aggcagtgaa gtggctcagg
     5401 cgctaagcga acaattctct ttagcaagcg aagcgagcga atcaaacgga gaagcagaag
     5461 cccattttat tcatttgaaa aatacaaaga tttactgtgg agacagtaaa tctactcctt
     5521 ctaaaggcaa aatcttttgg agagggaaaa tagcagaagt agacgggttt ttcttaggaa
     5581 agatttctga tgcaaaatca acgagtaaaa agagttcata aaaaacggcg gggtgattgc
     5641 cccgccgttt tttagtgatg tgatgagatg tgcagcttct tttttcgata catgagcgga
     5701 gtcatcttca tttcttttcg aaactgatta ataaaatagc ttgtactatt aaaccctact
     5761 tgataggcga cttccgtcac atttgcttct gcttgctgca gtccggattc gcgaacgcgt
     5821 ctcgagagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     5881 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     5941 gaaaggagga actatatccg actagtagcc cgcctaatga gcgggctttt ttttaattcc
     6001 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6061 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6121 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctc
     6181 gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag tgggatacat agaactggac
     6241 ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag agcgttttcc aatgatgagc
     6301 actttcaaag ttctgctatg tggagcagta ttatcccgtg tagatgcggg gcaagagcaa
     6361 ctcggacgac gaatacacta ttcgcagaat gacttggttg aatactcccc agtgacagaa
     6421 aagcacctta cggacggaat gacggtaaga gaattatgta gtgccgccat aacgatgagt
     6481 gataacactg cggcgaactt acttctgaca accatcggtg gaccgaagga attaaccgct
     6541 tttttgcaca atatgggaga ccatgtaact cgccttgacc gttgggaacc agaactgaat
     6601 gaagccatac caaacgacga gcgagacacc acaatgcctg cggcaatggc aacaacatta
     6661 cgcaaactat taactggcga actacttact ctggcttcac ggcaacaatt aatagactgg
     6721 cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg cacttcctgc tggctggttt
     6781 attgctgata aatctggggc aggagagcgt ggttcacggg gtatcattgc cgcacttgga
     6841 ccagatggta agccttcccg tatcgtagtt atctacacga cgggtagtca ggcaactatg
     6901 gacgaacgaa atagacagat tgctgaaata ggggcttcac tgattaagca ttggtaaacc
     6961 gatacaatta aaggctcctt ttggagcctt ttttttttgg acggaccggt agaaaagatc
     7021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7141 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
//
