LOCUS       sequence-446287-        9375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Matsuwaka M, Kumon M, Inoue A
  TITLE     H3K27 dimethylation dynamics reveal stepwise establishment of 
            facultative heterochromatin in early mouse embryos.
  JOURNAL   Nat Cell Biol. 2025 Jan;27(1):28-38. doi: 
            10.1038/s41556-024-01553-1. Epub 2024 Oct 31.
  PUBMED    39482357
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01553-1"; journalName: "Nat Cell Biol"; date: 
            "2025-01"; volume: "27"; issue: "1"; pages: "28-38"
COMMENT     Sequence Label: pcDNA3.1-Flag-Jarid2-polyA
FEATURES             Location/Qualifiers
     source          1..9375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             911..934
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             2194..2202
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4879..4908
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4924..4941
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4964..4981)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4970..5194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5240..5668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5327..5346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5537..5558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5677..5697)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5682..6011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5862..5997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5924..5943
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6078..6872
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6132..6151)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6742..6761
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7048..7169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7085..7104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7139..7158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7218..7234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7218..7234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7231..7253)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7242..7258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7266..7272,7273..7290,7291..7296))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7311..7332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7449..7466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7620..8208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7700..7719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8379..9170,9171..9239))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9002..9021
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9240..9344)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttatg gactacaaag acgatgacga caagggatcg agcaaggaaa gacccaagag
      961 gaatatcatt cagaagaaat acgatgacag cgatgggatc ccgtggtcag aagagagagt
     1021 tgtacgaaaa gtcctgtatt tgtccctaaa ggaattcaag aatgcacaga aaaggcagca
     1081 tggggaaggc cttgccggga gcctgaaggc ggtaaatggg cttcttggta atgcccaggc
     1141 taaggcacta ggaccagcct cagagcagtc agagaacgag aaggatgatg cctcccaagt
     1201 gtcctctact agcaacgatg ttagttcttc agattttgaa gaagggccgt cgaggaaaag
     1261 gcccaggctg caagcacaaa ggaagtttgc tcaatctcag ccgaatagtc ccagcacaac
     1321 tccagtgaag atagtggagc ctttgctacc cccgccagct actcaaattt ctgacctctc
     1381 taaaaggaag cctaagacag aagactttct tacctttctc tgccttcgag gttctcctgc
     1441 gctgcccaac agtatggtat attttggaag ctctcaggat gaggaggatg tcgaagagga
     1501 agatgatgag acggaagatg tcaaagcaac caccaacaat gcttcatctt cgtgccagtc
     1561 aacccccagg aaaggaaaaa cccataagca tgtacacaac gggcacgttt tcaatggctc
     1621 cagtaggtca gcacgggaga aagagcctgc tcacaaacac agaagcaaag aggccactcc
     1681 ggggaaggag aagcacagcg agcccagggc agacagccgg agagaacagg cttcaggggc
     1741 tcagcccacg gccgcctcgg ctgcggcttc ctctgccaag ggacttgctg ccaaccacca
     1801 acctccccct tcgcatcggt ctgctcagga cttacggaaa caggtgtcta aggtaaatgg
     1861 agtcactcga atgtcatctc tgggtgcagg tacaaacagt gccaaaaaga tccgagaagt
     1921 cagaccttca ccatccaaaa ctgtgaagta cactgccaca gtgaccaagg ggactgtcac
     1981 atacaccaaa gccaagagag aactggtcaa ggaaaccaaa cccaaccacc acaaacccag
     2041 ttcagctgtc aaccacacaa tctcagggaa aactgaaagt agcaatgcaa aaacccgcaa
     2101 acaggtgcta tcccttgggg gggcgtccaa gtccaccggg cctgctgcca gtggcctcaa
     2161 ggccagcagc aggttgaacc caaagtcatg cactaaggag gtgggggggc ggcagctgag
     2221 ggaggggctg cggaattcca aaaggagact ggaagaggca cagcaagtgg acaagccgca
     2281 gtcacccccc aagaagatga agggggtggc aggcaatgct gaagcccctg gcaaaaaggc
     2341 ctcagcggct tcaggagaaa agtctctgct gaatggacac gtgaagaagg aagtgcccga
     2401 gcgaagtttg gaaaggaatc ggccaaagcg ggccgcggct ggcaagaata tgctgggcaa
     2461 acaagcacat ggcaagacag agggcacccc ctgtgaaaat cgttctacct cgcaacccga
     2521 gtcctcgcac aagccgcatg acccacaggg caagccagag aaggggagcg gcaagtctgg
     2581 gtgggcagcg atggacgaga tccctgtcct caggccctcc gccaaggagt tccacgaccc
     2641 gctcatctac atcgagtcag tacgtgctca ggtggagaag tacgggatgt gtcgcgtgat
     2701 tccccctcca gactggcggc cagagtgcaa gctcaacgat gagatgcgct ttgtcacgca
     2761 gatccagcac atccataagc tgggccggcg ctggggcccc aacgtgcagc ggctagcctg
     2821 cataaagaag cacctcagat ctcagggcat caccatggat gagctccccc tcataggagg
     2881 ctgtgagctc gacctggcct gctttttccg gctgattaat gagatgggcg gcatgcagca
     2941 agtgactgac ctcaaaaaat ggaacaaact agcagacatg ctgcgcattc ccaaaactgc
     3001 ccaggaccgg ctggccaagc tgcaggaggc ctactgccag tacctgcttt cctatgactc
     3061 cctgtccccc gaggagcacc gacggctgga gaaggaggtg ctgatggaga aggagatcct
     3121 ggaaaagcgc aaagggccat tagaggggca cacggagagc gaccaccaca agttccactc
     3181 cctgccccgc tttgagccca agaatgggct cgtccacggt gtcacaccta ggaatggatt
     3241 ccgtagcaag ctcaaggagg tgggccgggc cccactgaag acgggccggc ggcggctgtt
     3301 cgctcaggaa aaagaagtgg tcaaagagga ggaagaagac aaaggtgtcc tcaatgactt
     3361 ccacaagtgc atctataagg gaagatctgt ttctctaaca actttttatc gaacagcaag
     3421 aaacatcatg aacatgtgtt tcagcaagga gcccgcacca gcagagattg agcaagagta
     3481 ctggaggtta gtggaagaga aggactgtca tgtggcagtt cactgcggaa aggtggacac
     3541 taatacccat ggcagcgggt tcccggtggg aaaatcagaa cccttttcaa ggcatggatg
     3601 gaacctcact gtcctcccaa ataacacagg gtccatcctg cgtcaccttg gtgctgtgcc
     3661 tggagtgact attccctggc taaatattgg catggtcttt tctacctcat gctggtctcg
     3721 agaccaaaat caccttccat atattgacta cttacacact ggtgctgact gcatttggta
     3781 ttgcattcct gctgaggagg agaacaagct ggaggacgta gtccacactc tgctgcaggg
     3841 caatggtacc ccagggctgc agatgctgga gagcaatgta atgatctccc cagaggtgct
     3901 gtgcaagaag gggatcaagg tccacaggac tgtacagcag agtgggcagt ttgtcgtctg
     3961 cttcccggga tcctttgtgt ccaaagtatg ttgtggctac aacgtctcag agacagtgca
     4021 ctttgctacc acccagtgga caagcatggg cttcgagact gccaaggaga tgaagcgccg
     4081 ccatatagct aagccattct ccatggagaa gttgctctac cagattgcac aagcagaagc
     4141 aaaaaaggaa aatggtccca ccctcagtac catctcagcc cttctggatg agctcaggga
     4201 tacagagctg cggcagcgca ggctgttgtt cgaggctggc cttcactctt ctgcacgcta
     4261 tggcagccac gacggcaaca gcacagtggc ggatgggaag aaaaagcctc gaaagtggct
     4321 acagctggaa acttcagaaa ggaggtgcca gatctgccag cacttgtgct acctgtccat
     4381 ggtggtacag gagaatgaga atgtcgtgtt ctgcctggag tgtgcactgc gccatgtgga
     4441 gaagcagaag tcttgccgtg ggctgaagct gatgtaccgc tacgatgagg agcagatcat
     4501 cagtttggtc aaccagatat gtggcaaagt gtctggcaaa cacggtggca tcgagaactg
     4561 tctcaacaaa cccacaccaa aaagaggtcc ccgcaagaga gcgaccgtgg atgtgccgcc
     4621 atctcggctc ccatcctcat gaaattctgc agatatccat cacactggcg gccgctcgac
     4681 tatgggcacc aaagaacctg taaacgttat ctttttaaat tgaatgtgca caaataaaag
     4741 tttggaaaag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaatacgcat catacgctgt ggcgtacctc
     4861 gagtctagag ggcccttcga acaaaaactc atctcagaag aggatctgaa tatgcatacc
     4921 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     4981 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5041 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5101 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5161 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5221 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5641 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5761 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5821 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5941 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6001 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6061 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6121 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6181 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6241 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6301 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6361 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6421 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6481 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6541 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6601 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6661 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6721 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6781 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6841 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc
     6901 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6961 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7021 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7081 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7141 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7201 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7261 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7321 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7381 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7441 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7501 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7561 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8341 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8401 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8461 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8521 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8581 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8641 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8701 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8761 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8821 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8881 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8941 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9001 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9061 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9361 gtgccacctg acgtc
//
