LOCUS       sequence-44629-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Liu M, Horowitz A
  TITLE     A PDZ-binding motif as a critical determinant of Rho guanine 
            exchange factor function and cell phenotype.
  JOURNAL   Mol Biol Cell. 2006 Apr;17(4):1880-7. Epub 2006 Feb 8.
  PUBMED    16467373
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2006-04"; volume: "17(4)"; pages: "1880-7. Epub 2006 
            Feb 8"
COMMENT     Sequence Label: pcDNA4.1/HisMax-Syx1
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    104..266
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             279..296
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..332
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(336..344,345..359)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttagcgcag aggcttgggg
      121 cagccgagcg gcagccaggc cccggcccgg gcctcggttc cagaagggag aggagcccgc
      181 caaggcgcgc aagagagcgg gctgcctcgc agtccgagcc ggagagggag cgcgagccgc
      241 gccggccccg gacggcctcc gaaaccatgg ggggttctca tcatcatcat catcatggta
      301 tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg
      361 taccaggatc tgggacagga ccgggcgtct ccgggcgccg agcagcagcc aggcccagct
      421 ctgagctacc ctccccagac tcccagctcc tctgggtcgg gggtcatgcc cacagcagcg
      481 acagccaggt atgccatcat gccgactgcc agcagctgca ccaccggggg cctctcaacc
      541 tgtgtgagac ctgtgatagc aagttccaca gcaccctgca ctacgacggc catgtccgat
      601 ttgacctgcc cccgcaaggc tctgtcctgg cccggaatgt ttccacccgg tcctgtcctc
      661 cccgcaccag ccctgctgca gacctggagg aagaagagga aggctgtacc gacggaaaag
      721 gggaccggaa gagcgccggc ctaaaaatct ctaagaagaa agcag
//
