LOCUS       sequence-446480-       19754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, 
            kanamycin and red fluorescent seed selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19754)
  TITLE     Takeuchi CRISPR/Cas9 Plasmids for multi-gene knockout in Arabidopsis
REFERENCE   2  (bases 1 to 19754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHEE-R-kan5A
FEATURES             Location/Qualifiers
     source          1..19754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             531..1238
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     944..963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3124..3199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5076..5141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5148..5168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5193..9293
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5193..9293
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9206..9214
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9294..9341
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(9987..10003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9987..10003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10000..10022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10011..10027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10035..10041,10042..10059,10060..10065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10080..10101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10292..10968
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10905..10925
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11031..11828
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11088..11107)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11698..11717
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11885..12059
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12137..12161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12586..13380
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14805..15393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15294..15313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15547..15564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15579..15719
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15645..15664)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15805..15827
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16063..16257
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16323..17396)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17825..18454)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc ttgaaaccaa attaacatag
       61 ggtttttatt taaataaaag tttaaccttc tttttaaaaa attgttcata gtgtcatgtc
      121 agaacaagag ctacaaatca cacatagcat gcataagcgg agctatgatg agtggtattg
      181 ttttgttcgt cacttgtcac tcttttccaa cacataatcc cgacaacaac gtaagagcat
      241 ctctctctct ccacacacac tcatgcatgc atgcattctt acacgtgatt gccatgcaaa
      301 tctcctttct cacctataaa tacaaaccaa cccttcacta cactcttcac tcaaaccaaa
      361 acaagaaaac atacacaaat agcaaaacaa gggcgaattc tcaacacaac atatacaaaa
      421 caaacgaatc tcaagcaatc aagcattcta cttctattgc agcaatttaa atcatttctt
      481 ttaaagcaaa agcaattttc tgaaaatttt caccatttac gaacgatagc atggtgagca
      541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1201 gccgccactc caccggcggc atggacgagc tgtacaagaa ggacgagctg taagcgccgc
     1261 ccggctgcat cgctgaaatc accagtctct ctctacaaat ctatctctct ctataataat
     1321 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1441 ctaattccta aaaccaaaat ccagtggtac caagcttcga cttgccttcc gcacaataca
     1501 tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag
     1561 cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt
     1621 ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga
     1681 atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct
     1741 gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta
     1801 tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa
     1861 cgaagctgag tttatataca gctagagtcg aagtagtgat tgggagacca acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     3001 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     3061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc
     3121 tcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3181 agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct
     3241 gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat
     3301 ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg
     3361 agttttgcag ttccgatgag ataaaccaat accatggtta tactagtctc aacttttgat
     3421 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtgacactg tttgcactgg
     3481 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
     3541 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
     3601 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
     3661 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
     3721 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgtttca gccatatcac
     3781 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
     3841 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
     3901 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
     3961 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
     4021 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
     4081 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
     4141 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
     4201 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
     4261 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
     4321 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     4381 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     4441 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     4501 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     4561 tgatcactga cttggttcta accaaaaaaa aaaaaatgtt taatttacat atctaaaagt
     4621 aggtttaggg aaacctaaac agtaaaatat ttgtatatta ttcgaatttc actcatcata
     4681 aaaacttaaa ttgcaccata aaattttgtt ttactattaa tgatgtaatt tgtgtaactt
     4741 aagataaaaa taatattccg taagttaacc ggctaaaacc acgtataaac cagggaacct
     4801 gttaaaccgg ttctttactg gataaagaaa tgaaagccca tgtagacagc tccattagag
     4861 cccaaaccct aaatttctca tctatataaa aggagtgaca ttagggtttt tgttcgtcct
     4921 cttaaagctt ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag
     4981 gtgatcttct ttctccaaat cctctctcat aactctgatt tcgtacttgt gtatttgagc
     5041 tcacgctctg tttctctcac cacagcctct agatggatta caaggaccac gacggggatt
     5101 acaaggacca cgacattgat tacaaggatg atgatgacaa gatggctccg aagaagaaga
     5161 ggaaggttgg catccacggg gtgccagctg ctgacaagaa gtactcgatc ggcctcgata
     5221 ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga
     5281 agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc
     5341 tcctgttcga cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc
     5401 ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg
     5461 cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata
     5521 agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga
     5581 agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc
     5641 tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg
     5701 agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga
     5761 cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta
     5821 tcctgtccgc taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg
     5881 gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca
     5941 acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat
     6001 acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc
     6061 tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg
     6121 agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg
     6181 atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct
     6241 tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg
     6301 agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg
     6361 tgaagctcaa tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc
     6421 cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc
     6481 ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact
     6541 acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg
     6601 agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt
     6661 tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc
     6721 actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga
     6781 ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc
     6841 tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga
     6901 agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc
     6961 tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg
     7021 agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga
     7081 tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc
     7141 tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc
     7201 gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc
     7261 gcaatttcat gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg
     7321 ctcaggtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc
     7381 cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca
     7441 tgggcaggca caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc
     7501 agaagggcca gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc
     7561 tggggtccca gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc
     7621 tctacctgta ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta
     7681 acaggctcag cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact
     7741 ccattgacaa caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc
     7801 cttcagagga ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc
     7861 tgatcacgca gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc
     7921 tggacaaggc gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg
     7981 ttgcgcagat tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc
     8041 gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc
     8101 agttctacaa ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg
     8161 cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg
     8221 gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca
     8281 aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca
     8341 ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg
     8401 agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac
     8461 aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc
     8521 tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt
     8581 acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga
     8641 agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc
     8701 gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga
     8761 agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga
     8821 agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca
     8881 agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg
     8941 acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg
     9001 agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc
     9061 tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc
     9121 atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta
     9181 tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt
     9241 cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc gacaagcggc
     9301 cagcggcgac gaagaaggcg gggcaggcga agaagaagaa gtgagctcag agctttcgtt
     9361 cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca
     9421 ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt
     9481 tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg
     9541 aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat
     9601 tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac
     9661 attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag
     9721 gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca
     9781 gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt
     9841 tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc attgctttat
     9901 aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta
     9961 tgacttgcca attgggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10021 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10081 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10141 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10201 ggctagagca gcttgccaac atggtggagc acgacactct cgtctactcc aagaatatca
    10261 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
    10321 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
    10381 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
    10441 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
    10501 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac
    10561 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    10621 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    10681 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    10741 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
    10801 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    10861 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    10921 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct
    10981 ctctctacaa atctatctct ctcgagcttt cgcagatctg tcgatcgacc atggggattg
    11041 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
    11101 actgggcaca acagacaatc ggctgctctg atgccgccgt gtttcggctg tcagcgcagg
    11161 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaggacg
    11221 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
    11281 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
    11341 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
    11401 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
    11461 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
    11521 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
    11581 atctcgtcgt gacacatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
    11641 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
    11701 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
    11761 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
    11821 tcttctgagc gggactctgg ggttcggatc gatcctctag ctagagtcga tcgacaagct
    11881 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
    11941 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
    12001 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc
    12061 cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
    12121 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
    12181 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
    12241 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
    12301 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
    12361 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc
    12421 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
    12481 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc
    12541 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
    12601 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
    12661 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
    12721 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
    12781 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
    12841 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
    12901 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
    12961 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
    13021 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt
    13081 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
    13141 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
    13201 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
    13261 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
    13321 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
    13381 tacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgctgaga
    13441 gatcccctca taatttcccc aaagcgtaac catgtgtgaa taaattttga gctagtaggg
    13501 ttgcagccac gagtaagtct tcccttgtta ttgtgtagcc agaatgccgc aaaacttcca
    13561 tgcctaagcg aactgttgag agtacgtttc gatttctgac tgtgttagcc tggaagtgct
    13621 tgtcccaacc ttgtttctga gcatgaacgc ccgcaagcca acatgttagt tgaagcatca
    13681 gggcgattag cagcatgata tcaaaacgct ctgagctgct cgttcggcta tggcgtaggc
    13741 ctagtccgta ggcaggactt ttcaagtctc ggaaggtttc ttcaatctgc attcgcttcg
    13801 aatagatatt aacaagttgt ttgggtgttc gaatttcaac aggtaagtta gttgctagaa
    13861 cccatggctc ctttgccgac gctgagtaga ttttaggtga cgggtggtga caatgagtcc
    13921 gtgtcgagcg ctgatttttt cggcctttag agcgagattt atacaataga atttggcatg
    13981 agattggatt gcttttagtc agcctcttat agcctaaagt ctttgagtga ctagatgaca
    14041 tatcatgtaa gttgctgata ggtttccagt tttccgctcc taggtctgca tattgtactt
    14101 ttcctcttac tcgacttaac cagtaccaac ccagcttctc aacggattta taccatggca
    14161 ctttaaagcc agcatcactg acaatgagcg gtgtggtgtt actcggtaga atgctcgcaa
    14221 ggtcggctag aaattggtca tgagctttct ttgaacattg ctctgaaagc gggaacgctt
    14281 tctcataaag agtaacagaa cgaccgtgta gtgcgactga agctcgcaat accataagtc
    14341 gtttttgctc acgaatatca gaccagtcaa caagtacaat gggcatcgta ttgcccgaac
    14401 agataaagct agcatgccaa cggtatacag cgagtcgctc tttgtggagg tgacgattac
    14461 ctaacaatcg gtcgattcgt ttgatgttat gttttgttct cgctttggtt ggcaggttac
    14521 ggccaagttc ggtaagagtg agagttttac agtcaagtaa tgcgtggcaa gccaacgtta
    14581 agctgttgag tcgttttaag tgtaattcgg ggcagaattg gtaaagagag tcgtgtaaaa
    14641 tatcgagttc gcacatcttg ttgtctgatt attgattttt cgcgaaacca tttgatcata
    14701 tgacaagatg tgtatccacc ttaacttaat gatttttacc aaaatcatta ggggattcat
    14761 cagcactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    14821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    14881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    14941 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    15001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    15061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    15121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    15181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    15241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    15301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    15361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    15421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    15481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    15541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
    15601 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
    15661 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
    15721 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
    15781 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
    15841 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
    15901 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
    15961 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
    16021 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
    16081 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
    16141 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
    16201 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
    16261 aacagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
    16321 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
    16381 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
    16441 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
    16501 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
    16561 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
    16621 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
    16681 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
    16741 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
    16801 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
    16861 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
    16921 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
    16981 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
    17041 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
    17101 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
    17161 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
    17221 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
    17281 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
    17341 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
    17401 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta
    17461 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
    17521 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
    17581 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
    17641 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
    17701 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
    17761 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
    17821 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
    17881 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
    17941 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
    18001 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
    18061 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
    18121 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
    18181 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
    18241 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
    18301 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
    18361 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
    18421 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
    18481 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
    18541 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
    18601 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
    18661 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
    18721 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    18781 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    18841 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    18901 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    18961 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    19021 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    19081 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    19141 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    19201 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    19261 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    19321 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    19381 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    19441 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    19501 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    19561 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    19621 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
    19681 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
    19741 ctcttttctc ttag
//
