LOCUS       sequence-446792-       14855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under 
            the spleen focus-forming virus (SFFV) promoter and Emerald under the
            Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14855)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-KTN1-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..14855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6607..6627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7025..7036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8075..8094
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      8268..8277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8274..8276,8277..8279,8280..8993)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8319..8340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8580..8599)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8927..8948
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9088..9321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9346..9364)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9347..9364)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9654..9673)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9887..9906)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10006..10028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10066..10084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10152..10256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10257..10325,10326..11117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10475..10494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11288..11876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11777..11796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12030..12047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12117..12137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12122..12451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12302..12437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13585..13650
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13780..13800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14225..14359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14262..14281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14316..14335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(14527..14855,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggagtttta tgagtcagca tattttattg ttcttattcc
     2461 ttcaatagtt attacagtaa ttttcctctt cttctggctt ttcatgaaag aaacattata
     2521 tgatgaagtt cttgcaaaac agaaaagaga acaaaagctt attcctacca aaacagataa
     2581 aaagaaagca gaaaagaaaa agaataaaaa gaaagaaatc cagaatggaa acctccatga
     2641 atccgactct gagagtgtac ctcgagactt taaattatca gatgctttgg cagtagaaga
     2701 tgatcaagtt gcacctgttc cattgaatgt cgttgaaact tcaagtagtg ttagggaaag
     2761 aaaaaagaag gaaaagaaac aaaagcctgt gcttgaagag caggtcatca aagaaagtga
     2821 cgcatcaaag attcctggca aaaaagtaga acctgtccca gttactaaac agcccacccc
     2881 tccctctgaa gcagctgcct cgaagaagaa accagggcag aagaagtcta aaaatggaag
     2941 cgatgaccag gataaaaagg tggaaactct catggtacca tcaaaaaggc aagaagcatt
     3001 gcccctccac caagagacta aacaagaaag tggatcaggg aagaagaaag cttcatcaaa
     3061 gaaacaaaag acagaaaatg tcttcgtaga tgaacccctt attcatgcaa ctacttatat
     3121 tcctttgatg gataatgctg actcaagtcc tgtggtagat aagagagagg ttattgattt
     3181 gcttaaacct gaccaagtag aagggatcca gaaatctggg actaaaaaac tgaagaccga
     3241 aactgacaaa gaaaatgctg aagtgaagtt taaagatttt cttctgtcct tgaagactat
     3301 gatgttttct gaagatgagg ctctttgtgt tgtagacttg ctaaaggaga agtctggtgt
     3361 aatacaagat gctttaaaga agtcaagtaa gggagaattg actacgctta tacatcagct
     3421 tcaagaaaag gacaagttac tcgctgctgt gaaggaagat gctgctgcta caaaggatcg
     3481 gtgtaagcag ttaacccagg aaatgatgac agagaaagaa agaagcaatg tggttataac
     3541 aaggatgaaa gatcgaattg gaacattaga aaaggaacat aatgtatttc aaaacaaaat
     3601 acatgtcagt tatcaagaga ctcaacagat gcagatgaag tttcagcaag ttcgtgagca
     3661 gatggaggca gagatagctc acttgaagca ggaaaatggt atactgagag atgcagtcag
     3721 caacactaca aatcaactgg aaagcaagca gtctgcagaa ctaaataaac tacgccagga
     3781 ttatgctagg ttggtgaatg agctgactga gaaaacagga aagctacagc aagaggaagt
     3841 ccaaaagaag aatgctgagc aagcagctac tcagttgaag gttcaactac aagaagctga
     3901 gagaaggtgg gaagaagttc agagctacat caggaagaga acagcggaac atgaggcagc
     3961 acagcaagat ttacagagta aatttgtggc caaagaaaat gaagtacaga gtctgcatag
     4021 taagcttaca gataccttgg tatcaaaaca acagttggag caaagactaa tgcagttaat
     4081 ggaatcagag cagaaaaggg tgaacaaaga agagtctcta caaatgcagg ttcaggatat
     4141 tttggagcag aatgaggctt tgaaagctca aattcagcag ttccattccc agatagcagc
     4201 ccagacctcc gcttcagttc tagcagaaga attacataaa gtgattgcag aaaaggataa
     4261 gcagataaaa cagactgaag attctttagc aagtgaacgt gatcgtttaa caagtaaaga
     4321 agaggaactt aaggatatac agaatatgaa tttcttatta aaagctgaag tgcagaaatt
     4381 acaggccctg gcaaatgagc aggctgctgc tgcacatgaa ttggagaaga tgcaacaaag
     4441 tgtttatgtt aaagatgata aaataagatt gctggaagag caactacaac atgaaatttc
     4501 aaacaaaatg gaagaattta agattctaaa tgaccaaaac aaagcattaa aatcagaagt
     4561 tcagaagcta cagactcttg tttctgaaca gcctaataag gatgttgtgg aacaaatgga
     4621 aaaatgcatt caagaaaaag atgagaagtt aaagactgtg gaagaattac ttgaaactgg
     4681 acttattcag gtggcaacta aagaagagga gctgaatgca ataagaacag aaaattcatc
     4741 tctgacaaaa gaagttcaag acttaaaagc taagcaaaat gatcaggttt cttttgcctc
     4801 tctagttgaa gaacttaaga aagtgatcca tgagaaagat ggaaagatca agtctgtaga
     4861 agagcttctg gaggcagaac ttctcaaagt tgctaacaag gagaaaactg ttcaggattt
     4921 gaaacaggaa ataaaggctc taaaagaaga aataggaaat gtccagcttg aaaaggctca
     4981 acagttatct atcacttcca aagttcagga gcttcagaac ttattaaaag gaaaagagga
     5041 acagatgaat accatgaagg ctgttttgga agagaaagag aaagacctag ccaatacagg
     5101 gaagtggtta caggatcttc aagaagaaaa tgaatcttta aaagcacatg ttcaggaagt
     5161 agcacaacat aacttgaaag aggcctcttc tgcatcacag tttgaagaac ttgagattgt
     5221 gttgaaagaa aaggaaaatg aattgaagag gttagaagcc atgctaaaag agagggagag
     5281 tgatctttct agcaaaacac agctgttaca ggatgtacaa gatgaaaaca aattgtttaa
     5341 gtcccaaatt gagcagctta aacaacaaaa ctaccaacag gcatcttctt ttccccctca
     5401 tgaagaatta ttaaaagtaa tttcagaaag agagaaagaa ataagtggtc tctggaatga
     5461 gttagattct ttgaaggatg cagttgaaca ccagaggaag aaaaacaatg accttcggga
     5521 gaaaaactgg gaagcaatgg aagcattggc atcaactgaa aaaatgctgc aggacaaagt
     5581 gaacaagact tccaaggaaa ggcagcaaca ggtggaagct gttgagttgg aggctaaaga
     5641 agttctcaaa aaattatttc caaaggtgtc tgtcccttct aatttgagtt atggtgaatg
     5701 gttgcatgga tttgaaaaaa aggcaaaaga atgtatggct ggaacttcag ggtcagagga
     5761 ggttaaggtt ctagagcaca agttgaaaga agctgatgaa atgcacacat tgttacagct
     5821 agagtgtgaa aaatacaaat ccgtccttgc agaaacagaa ggaattttac agaagctaca
     5881 gagaagtgtt gagcaagaag aaaataaatg gaaagttaag gtcgatgaat cacacaagac
     5941 tattaaacag atgcagtcat catttacatc ttcagaacaa gagctagagc gattaagaag
     6001 cgaaaataag gatattgaaa atctgagaag agaacgagaa catttggaaa tggaactaga
     6061 aaaggcagag atggaacgat ctacctatgt tacagaagtc agagagctga aagatctgtt
     6121 gactgaattg cagaaaaaac ttgatgattc atattctgaa gcagtaagac agaatgaaga
     6181 gctaaatttg ttgaaggcac agttaaatga aacactcaca aaacttagaa ctgaacaaaa
     6241 tgaaagacag aaggtagctg gtgatttgca taaggctcaa cagtcactgg agcttatcca
     6301 gtcaaaaata gtaaaagctg ctggagacac tactgttatt gaaaatagtg atgtttcccc
     6361 agaaacggag tcttctgaga aggagacaat gtctgtaagt ctaaatcaga ctgtaacaca
     6421 gttacagcag ttgcttcagg cggtaaacca acagctcaca aaggagaaag agcactacca
     6481 ggtgttagag tgaagcggcc gcgactctag agtcgacctg caggcatgca agcttgatat
     6541 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6601 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6661 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6721 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6781 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6841 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6901 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6961 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7021 cccttcggcc ctcaatccag cggaccttcc ttcccgcggg cgcccccctc ctcacggcga
     7081 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
     7141 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
     7201 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
     7261 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
     7321 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
     7381 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc
     7441 tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga
     7501 gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg
     7561 ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg
     7621 ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga
     7681 ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg
     7741 gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc
     7801 ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt
     7861 cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta
     7921 ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc
     7981 ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt
     8041 tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc
     8101 tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt
     8161 gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt
     8221 ttggcttttt tgttagacga agcttggtac cgagctcgga tcaattcgcc accatggtga
     8281 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     8341 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     8401 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     8461 ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     8521 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     8581 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     8641 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     8701 agtacaacta caacagccac aaggtctata tcaccgccga caagcagaag aacggcatca
     8761 aggtgaactt caagacccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     8821 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     8881 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     8941 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taactcgagg
     9001 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     9061 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9121 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9181 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9241 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9301 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
     9361 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     9421 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     9481 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     9541 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     9601 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     9661 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     9721 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     9781 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     9841 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
     9901 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9961 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    10021 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
    10081 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
    10141 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    10201 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10261 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10321 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10381 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10441 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    10501 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10561 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10621 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10681 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10741 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10801 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10861 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10921 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10981 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    11041 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    11101 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    11161 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    11221 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11281 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11341 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11401 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11461 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11521 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11581 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11641 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11701 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11761 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11821 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11881 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    11941 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    12001 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    12061 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
    12121 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    12181 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    12241 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    12301 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    12361 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    12421 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
    12481 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
    12541 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
    12601 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
    12661 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
    12721 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
    12781 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
    12841 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
    12901 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
    12961 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
    13021 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
    13081 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
    13141 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
    13201 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
    13261 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
    13321 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
    13381 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
    13441 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
    13501 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
    13561 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
    13621 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
    13681 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
    13741 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
    13801 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
    13861 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
    13921 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
    13981 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
    14041 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
    14101 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
    14161 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    14221 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    14281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    14341 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    14401 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    14461 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    14521 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
    14581 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
    14641 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
    14701 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
    14761 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
    14821 cccgagagct gcatccggag tacttcaaga actgc
//
