LOCUS       sequence-446838-       12491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) 
            fused to myc-tag under the spleen focus-forming virus (SFFV) 
            promoter and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12491)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..12491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2423..2452
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4243..4263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4661..4672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5711..5730
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5904..5913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5910..5912,5913..5915,5916..6629)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5955..5976)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6216..6235)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6563..6584
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6724..6957
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6982..7000)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6983..7000)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7290..7309)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7523..7542)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7642..7664
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7702..7720)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7788..7892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7893..7961,7962..8753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8111..8130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8924..9512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9413..9432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9666..9683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9753..9773)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9758..10087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9938..10073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11221..11286
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11416..11436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11861..11995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11898..11917)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11952..11971
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12163..12491,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggaacaaaa actcatctca gaagaggatc tgatggccaa
     2461 gaaccgcagg gacagaaaca gttggggtgg attttcggaa aagacatatg aatggagctc
     2521 agaagaggag gagccagtga aaaaggcagg accagtccaa gtcctcattg tcaaagatga
     2581 ccattccttt gagttagatg aaactgcatt aaatcggatc cttctctcgg aggctgtcag
     2641 agacaaggag gttgttgctg tatctgttgc tggagcattt agaaaaggaa aatcattcct
     2701 gatggacttc atgttgagat acatgtacaa ccaggaatca gttgattggg ttggagacta
     2761 caatgaacca ttgactggtt tttcatggag aggtggatct gagcgagaga ccacaggaat
     2821 tcagatatgg agtgaaatct tccttatcaa taaacctgat ggtaaaaagg ttgcagtgtt
     2881 attgatggat actcagggaa cctttgatag tcagtcaact ttgagagatt cagccacagt
     2941 atttgccctt agcacaatga tcagctcaat acaggtatat aacttatccc aaaatgtcca
     3001 ggaggatgat cttcagcacc tccagctttt cactgagtat ggcagactgg caatggagga
     3061 aacattcctg aagccatttc agagtctgat atttcttgtt cgagactgga gtttcccata
     3121 cgaattttca tatggagccg atggtggtgc caaattcttg gaaaaacgcc tcaaggtctc
     3181 agggaaccag catgaagaac tacagaacgt cagaaaacac atccattcct gtttcaccaa
     3241 catttcctgt tttctgctac ctcatcctgg cttaaaagta gctaccaatc caaactttga
     3301 tggaaaattg aaagaaatag atgatgaatt catcaaaaac ttgaaaatac tgattccttg
     3361 gctacttagt cccgagagcc tagatattaa agagatcaat gggaataaaa tcacctgccg
     3421 gggtctggtg gagtacttca aggcttatat aaagatctat caaggtgaag aattaccaca
     3481 tcccaaatcc atgttacagg ccacagcaga agctaacaat ttagcagccg tggcaactgc
     3541 caaggacaca tacaacaaaa aaatggaaga gatttgtggt ggtgacaaac catttctggc
     3601 cccaaatgac ttgcagacca aacacctgca acttaaggaa gaatctgtga agctattccg
     3661 aggggtgaag aagatgggtg gggaagaatt tagccggcgt tacctgcagc agttggagag
     3721 tgaaatagat gaactttaca tccaatatat caagcacaat gatagcaaaa atatcttcca
     3781 tgcagctcgt accccagcca cactgtttgt agtcatcttt atcacatatg tgattgctgg
     3841 tgtgactgga ttcattggtt tggacatcat agctagccta tgcaatatga taatgggact
     3901 gacccttatc accctgtgca cttgggcata tatccggtac tctggagaat accgagagct
     3961 gggagctgta atagaccagg tggctgcagc tctgtgggac cagggaagta caaatgaggc
     4021 tttgtacaag ctttacagtg cagcagcaac ccacagacat ctgtatcatc aagctttccc
     4081 tacaccaaag tcggaatcta ctgaacaatc agaaaagaaa aaaatgtaaa gcggccgcga
     4141 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     4201 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4261 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4321 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4381 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4441 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4501 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4561 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4621 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4681 ccttccttcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     4741 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     4801 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     4861 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     4921 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     4981 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     5041 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     5101 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     5161 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     5221 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     5281 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     5341 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     5401 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     5461 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     5521 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     5581 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     5641 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     5701 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     5761 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     5821 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagct
     5881 tggtaccgag ctcggatcaa ttcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     5941 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     6001 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     6061 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt
     6121 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6181 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     6241 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     6301 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg
     6361 tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca
     6421 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     6481 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     6541 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     6601 ctctcggcat ggacgagctg tacaagtaac tcgagggaat taattcgagc tcggtacctt
     6661 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6721 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6781 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6841 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6901 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     6961 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     7021 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     7081 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     7141 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     7201 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
     7261 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     7321 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     7381 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     7441 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     7501 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     7561 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     7621 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     7681 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7741 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7801 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7861 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7921 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7981 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8041 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8101 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8161 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8221 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8281 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8341 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8401 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8461 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8521 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8581 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8641 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8701 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8761 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8821 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9061 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9601 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9661 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9721 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     9781 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9841 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9901 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9961 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10021 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    10081 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
    10141 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
    10201 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
    10261 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
    10321 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
    10381 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
    10441 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
    10501 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
    10561 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
    10621 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
    10681 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
    10741 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
    10801 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
    10861 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
    10921 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    10981 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    11041 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    11101 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    11161 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    11221 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    11281 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    11341 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    11401 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    11461 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    11521 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    11581 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    11641 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    11701 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    11761 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    11821 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    11881 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11941 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    12001 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    12061 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    12121 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
    12181 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
    12241 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
    12301 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
    12361 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
    12421 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
    12481 tcaagaactg c
//
