LOCUS       sequence-446857-        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Honnell V, Sweeney S, Norrie J, Parks M, Ramirez C, Jannu AJ, Xu B, 
            Teubner B, Lee AY, Bell C, Dyer MA
  TITLE     Evolutionary conservation of VSX2 super-enhancer modules in retinal 
            development.
  JOURNAL   Development. 2024 Jul 1;151(13):dev202435. doi: 10.1242/dev.202435. 
            Epub 2024 Jul 12.
  PUBMED    38994775
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-07-1"; volume: "151(13):dev202435. doi";
            pages: " 10.1242/dev.202435. Epub 2024 Jul 12"
COMMENT     Sequence Label: Vsx2 hR1-25 +SV40 base prom-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..102)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     312..329
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(523..1236,1237..1239,1240..1242))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(526..1236,1237..1239,1240..1242))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(568..589)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     917..936
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1176..1197
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1239..1248
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1283..1479)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1297..1432
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4835..4939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4940..5008,5009..5800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5158..5177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5971..6559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6460..6479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6713..6730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6798..6932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6822..6841)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6876..6895
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6938..7133)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6952..7087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7192..7213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7228..7233,7234..7251,7252..7258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7266..7282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7271..7293
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7290..7306
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7290..7306
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7649..7668
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7667..7722
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7768..7787
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(8282..9781)
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    join(9793..9872,1..473)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(9795..9872,1..496)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg
       61 ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac
      121 tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg
      181 tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt
      241 cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt
      301 caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa
      361 gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaaaataa
      421 catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg
      481 gggggaggga gagggggggg ggggggggcg gaattggccg ctttacttgt acagctcgtc
      541 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      601 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
      661 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      721 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
      781 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
      841 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      901 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      961 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     1021 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     1081 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     1141 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     1201 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc
     1261 ccgggcccgc ggtaccaagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     1321 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     1381 gtcagcgatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     1441 ttaggggcgg gactatggtt gctgactaat tgagatgcag atcgcagatc tcgagataat
     1501 tcattaaaca taacagtata tacaggtgta acatattcac ggaatctctt accactcctg
     1561 tcagcacaca gtaggcactt aataagtgct agcttttttt tgttctttgt ctctccagag
     1621 catccattaa tgatttgtgt tcacctgacc atcacccctt tacagtgagc ttaattagta
     1681 aggtacaatg tttgctaaat gaccgagacc aaggagagga aagatgtttc caagctttca
     1741 tggcggcgag gctaagggtc tctgttacca caggagatag ggaaaggtct tcctgtgcca
     1801 gggctgaagt agtgggtaca gattacaagg ggcggctagg gtggggtgag gggacagatc
     1861 tggcatggga gtggtggcct ttgacagtca gtgggagcgc agagctgtcc aggtggcccc
     1921 cagcctcacc ctgccctgtg ccgaggagag cgggaactct gttcagcctc ccaggaccat
     1981 ggctgggggc atgaactcag tgcccagggg ctgcctggag accccccttg ctttaggccc
     2041 tgcagtccca agacccaagg ggccctcttc ttcttggtct ctagttagag aactgtggtc
     2101 ttctcatctg gtgagctcca tatggcacca agggcacttt gtgagtgggg ctctgatccc
     2161 tggaaggtgc tgggcgatgg ggagggagtg gagaggaagt ggtagccaga tagcccggca
     2221 tccagcaacc gtgcgtccta gcagcccatt cacatttttc ttttctcttt cctgtttttt
     2281 tttttttttt tttttgagac agtctcactc tgttgtccag gctggagtgc agtggcacga
     2341 tctcagctca ctgcaacctc tgtttcccag gttcaagcga ttcttctgcc tcagcgtcct
     2401 gcgtagctgg gattacaggc acccgccacc tggctaattt ttgtattttt agtagaaaca
     2461 gggtttctcc atgttggcca ggctggtctc gaactcctga tctcaggtga tccactcgcc
     2521 tcggcctccc aaagtgctgg gattacagcc atgagccacc acgcctggcc agcccactca
     2581 catttttcaa gggtgcacta caggcttgag gcccgggggc tcagaatttg gggatacaat
     2641 acagcttcag cagttgttag gtgaggtggc cctgggtctt aaaccaccca tgtccactgt
     2701 tgccactagt aaagacattg aacatttacc atgtggcagg tgctccacaa gcatgatcct
     2761 actgaaccct cacaaagacc caggacagtg tttagaacaa aaaaaatttt cttcccaggg
     2821 atggaaactg tggctcaaaa acattgtctc tttctgggtt cacagaactc acaagtggcc
     2881 aggccagcat tggaagccag ctctgcctgc ccgcacaggc ctggacgccc agccacaacc
     2941 tggtgtcccc tctgcccagc tctcgccttg gctctcagta agtgcctgga gctggtcacc
     3001 acgccttgaa gctccgccct tctccgtggc ctccagttcc taccgcccca ctgccccaaa
     3061 gctgtctcac ctgcctctag gctctgtacc tcccttccag tccactcagc ttcccaggag
     3121 gtccccaagt accccagtgt ctggggacag tggtgggcag agggccccag ggcctctcag
     3181 ctgggcccat ggctgagcgc tgtggggagg cgggcgcagt gcacagagct gatcctaacc
     3241 aagtttgcca gcccagcttt tctcatggct ctcacacaat ggtctgcttt gtgcccacct
     3301 gccgataatg cttaattagg cgaggaggtg attaatttga ggtaattgac agctaattag
     3361 aggcccacag gctcattaac ttgacatctt gtcttcgtga attggctgag tagggtcagg
     3421 tgatgcaagc cgctgcttct ccctccccag ctctcccggc ccctgtaggc tgaggcgatc
     3481 acctagggga gggctgggcc gacccaggag ttcaggtggg gcagtcccaa ggccaagagt
     3541 agaggaagcc aaggcagtga gggaggaagg gtccggatgg aggtgtctgc cctgacctta
     3601 agagggagag agtgggcagg gagtgagggc ttgtgggtac ttcagcaaag acacgtaaga
     3661 gaagtggggt attaacaggg ccggagggcg ggagggagag ctggtgtcca tgaagggagg
     3721 agggatgagg ggaagcaggt gagtggggca gcctgttgga actaagcata ggctgtgagg
     3781 tgacacagac ctggggctgg atcctagctc tgctactaac cagctaggtg actgtgaacc
     3841 agtgatttaa tcgctaaacc tcaggcttct ccgctgtaga atgggaataa taatggtacc
     3901 tgcctcagga ggttgtcatg aagagtccat gaagcaccga agtaaaggac aaatctgtga
     3961 attgaatgag cagggtcagg ctgagcagga tttgtacccg ggggcctgat gagtgctcaa
     4021 tcaaatgtta actagtccat taaaattttt taaaataaaa agttaactat tcttggtggc
     4081 aggaaggagt gacaagaaga gagagtgatt gggtggaatg ggccccagtg ctgtggggag
     4141 gcaatggcaa ccccctccca agataagcca ccgttaccct gcaggacacc tgcaggcctg
     4201 atcacagtgg cctggtggaa ggtgtgggga aaagaccttc ttgttgttgc catctctttt
     4261 gggcacgggg catgcaattc agcaatatat attaaagttt aaaatgcatg tggtctttga
     4321 cccagcaatt cccctgctag tgatatatcc acagatacct aaacacaagg gtatctgctc
     4381 atcaccacat agttcgtagt agcagaagac aggaaatgac ctgaatgtcc atcacagggc
     4441 ctggctaggt aactgaaggc acatccatat agtggaatag tatgtagctg ttacatctct
     4501 aggtaccaat atgaacaaga tccaagataa aaaaaaaagc aagctgcaga acaggtgtag
     4561 tttccacaag ctgttaacta gtggtctgag ggttatggga tggcaagaag atgtattttt
     4621 tcataatctg ttcattttaa atatagtttg tatttactac cttttcaaaa agtatttaaa
     4681 aagtcatact tttttttccc aaatgaaatc taacttgaaa tctgaattta taggcaaagt
     4741 gcctctggtt gaactggggg tgggggccca gtcctaccca cctatttcct tcctcccctc
     4801 cgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5161 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6181 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6241 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6301 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6361 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6421 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6481 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6541 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6601 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6661 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6721 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6781 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
     6841 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6901 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
     6961 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
     7021 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
     7081 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
     7141 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7201 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7261 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7321 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     7381 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     7441 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     7501 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     7561 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     7621 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     7681 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     7741 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct cagtggtatt
     7801 tgtgagccag ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag
     7861 gagtgcggcc ggccgcgata tcctcgaccc cactgggctg taggtggtgg ctggatggcc
     7921 tgagccccct cctttcctgg ggtgcaggca gccacagggc acaacctgca gtccctgggg
     7981 ttggggtagg gagtccgaga gcagccaagg ggcaggtttg gtgtcatggg ctcagtcccc
     8041 agagcgcacg gggaggggaa ggttccatgg caggacctct gtatagggac tccggggatg
     8101 acgactcggg ctggaggcca ggcaggacga caggaggtgg ggagcaggag aactccggga
     8161 tggggaagca ggagccccag ggacgggaca ctcagggagc agtggccgtc tccagcagca
     8221 gcagggtccc ggccagcaga ggaagcaacg cggggaccac ggaccgcccc gggtgcgcgg
     8281 cgtcggtggt gccggcgggg ggcgccaggt cgcaggcggt gtagggctcc aggcaggcgg
     8341 cgaaggccat gacgtgcgct atgaaggtct gctcctgcac gccgtgaacc aggtgcgcct
     8401 gcgggccgcg cgcgaacacc gccacgtcct cgcctgcgtg ggtctcttcg tccaggggca
     8461 ctgctgactg ctgccgatac tcggggctcc cgctctcgct ctcggtaaca tccggccggg
     8521 cgccgtcctt gagcacatag cctggaccgt ttccgtatag gaggaccgtg taggccttcc
     8581 tgtcccgggc cttgccaggg gccagcccga agatggagct ccctcgcagg gggtagcctc
     8641 cgaaggagaa gacgtgggag tggtcggcag tgacgaggct cagcgtgtcc tcctcgctgg
     8701 tgagctggcc cgccctctca atggcgtcgt cgaacatgat cgtctcagtc agtgcccggt
     8761 aagccctgct ttcatgatga ccatggtcga tgcgaccacc ctccacgaag aggaagaagc
     8821 cgcgggggtt cctgctcagc aggcgcaggg cagcctctgt catctccatc agggaggggt
     8881 ccagtgtgga gtctcggtgg atctcgtatt tcatgtctcc aggctcaaag agacccatga
     8941 gatgggtcac agacgggtcc agggaagcct gcatgagctc agtgcggttc cacacatacc
     9001 gggcaccctg gcgcttcgcc agccattcct gcaccagatt cttcccgtcc agcctggtcc
     9061 caccttggct gtagtcatct gggtactcag ggtctggggt tcccatgcga aacatgtact
     9121 ttcggcctcc acctaggatc acgtcaatgt ccatgttgga gatgagctgc gtagcgatgt
     9181 cctggcaccc ctcctggcgg gccgaggcag gcacgtcggc gtccgagtac cagttgcggt
     9241 tcaccgtgtg ggcgtaggtg ccggctggcg aggcgtgctg cactcgtgtg gtggttacca
     9301 ctcccactga cttccctgct ttcttggccc gattcatcac ggagatgacc tcgttgccgc
     9361 gtgtcgtgtt gcactggtta aagcgggcgg ctgcactcaa gccaatggtc tggaagttgc
     9421 ccttgacccc gcacaggtag gccgtggctg tggctccact gtctggcaca tgtttgtcta
     9481 cattgtatgt cttggacaga gccacatatg ggaagcggtc catggccagg ggtatctcag
     9541 gccccagttt gtccttcttc tgccctttta ggatcctggc agctgtcacc gtagacaccc
     9601 ccatcccatc gcccaggaag atgatgaggt tcttggcggc tgtctgtgca ggctgcagct
     9661 tcttggcggc acccagggcc tcggctgcct cgcggttcca gaagtccggg ttctcctcct
     9721 caactgggat gatgcccagg gagagctgta gcctcaggcc cagcagcagc agcagcagca
     9781 gcctcgaccc catattatca tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg
     9841 gggcctagac gtttttttaa cctcgactaa ac
//
