LOCUS       sequence-44717-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, 
            Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, 
            Hannon GJ, Lowe SW
  TITLE     A rapid and scalable system for studying gene function in mice using
            conditional RNA interference.
  JOURNAL   Cell. 2011 Apr 1;145(1):145-58.
  PUBMED    21458673
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-04-1"; volume: "145(1)"; pages: "145-58"
COMMENT     Sequence Label: pTGMP-p16/p19.478
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..117)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(114..152)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(184..202,203..225,226..244,245..267,268..286,287..309,
                     310..328,329..351,352..370,371..393,394..412,413..435,
                     436..454)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(518..534)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(550..572)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ctctcgccct tgctcaccat ggtggtcgag aggctggatc ggtcccggtg tcttctatgg
       61 aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg
      121 cttatatagg cctcccaccg tacacgccta cctcgacccg ggtaccgagc tcgactttca
      181 ctttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
      241 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
      301 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
      361 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
      421 ctcgactttc acttttctct atcactgata gggagtggta aactcgatgc tctagaggat
      481 cgatccccca gatctaattc cggcgcctag agaaggagtg agggctggat aaagggagga
      541 tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg gaggaagagg
      601 aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa aagccaaggc
      661 ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac
      721 cttgatctta acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac
      781 gttaaaggtt ggccattctg cagagc
//
