LOCUS       sequence-447285-       11450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT1G48590.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT1G48590-mKOk
FEATURES             Location/Qualifiers
     source          1..11450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1297..1321)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2302..2320)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2303..2320)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2349..2358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2388..2407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2389..2407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2414..2431)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2414..2430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2423..2445)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2588..2771
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2772..2813,2814..2822,2823..3593)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2853..2872)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3463..3482
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4218..4470
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4563..4587
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5108..5899
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6145..6733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6634..6653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6887..6904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6919..7059
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6985..7004)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7145..7167
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7403..7597
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7663..8736)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9165..9794)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11125..11149
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11314..11335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11350..11355,11356..11373,11374..11380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11388..11404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11393..11415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11412..11428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11412..11428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcatgat cgtttcattt ctctattttt
      721 cttcactttt tcctactagc ttgagaaaaa ttaatcttgt tgcaggggag aagcataaag
      781 atcggaggat gaaaccttct ttaatggata gtctactagg tctcctccgt attcgcatca
      841 aacgcggcgt caacctcgca gtccgtgatc tcaacagtag tgatccttac gttgtcgtca
      901 aaatggcaaa acagaaattg aagactcgag ttatttacaa aaatgtaaat cctgagtgga
      961 atgaagactt gactctctca gtttcagacc ccaatcttac cgtcctcttg acggtgtatg
     1021 attatgacac gttcaccaaa gacgacaaga tgggagatgc agaattcggg attaagccgt
     1081 tcgttaatgc gttgaagatg cacttacatg atctcccatc tgggaccatc gtaaccacgg
     1141 tccagcctag ccgcgacaac tgcctagccg aggagtctcg ggttatatgg tctgatggta
     1201 agctcgttca agatatagtt cttaggctga gacatgtcga atgtggtgag gttgaggctc
     1261 aacttcaatg gattgatctt cccggaaagg gtctagaccc agctttcttg tacaaagtgg
     1321 tgatgggacg tccgcggaga tctacgcgtg tcgactcgag atatccaact agtatggtga
     1381 gcgttatcaa gcctgagatg aagatgaggt actacatgga cggaagcgtg aacggacacg
     1441 agttcactat tgaaggcgaa ggtactggaa ggccttacga gggacatcaa gagatgactc
     1501 tcagagtgac catggctgaa ggtggaccta tgcctttcgc tttcgatctc gtgtctcacg
     1561 tgttctgcta cggacacaga gtgttcacta agtaccctga agagatcccg gactacttca
     1621 agcaagcttt ccctgaggga ctctcttggg agagatcttt ggaattcgag gacggtggat
     1681 ccgcttctgt gtctgctcat atctctctca ggggcaacac cttctaccac aagagcaagt
     1741 ttaccggggt gaacttccct gctgatggac cgatcatgca gaaccagtct gttgattggg
     1801 agccgtctac tgagaagatc accgcttctg atggtgtgct caagggtgat gtgaccatgt
     1861 accttaagct cgaaggtggt ggaaaccaca agtgccagtt caagactacc tacaaggccg
     1921 ccaaagaaat cctcgagatg cctggtgatc actacatcgg acataggctc gtgagaaaga
     1981 ccgagggaaa catcactgag caggttgagg atgctgtggc tcattctcaa ttgttataag
     2041 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     2101 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     2161 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     2221 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag gcatgcaagc
     2281 tagcttacta gtgatgcata ttctatagtg tcacctaaat ctgcggccgc actagtgata
     2341 tcccgcggcc atggcggccg ggagcatgcg acgtcgggcc caattcgccc tatagtgagt
     2401 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2461 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2521 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag
     2581 cgttaatggg tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc
     2641 gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca
     2701 ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca
     2761 aataatctgc aatggcaatt accttatccg caacttcttt acctatttcc gcccggatcc
     2821 gggcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2881 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2941 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     3001 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     3061 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     3121 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     3181 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     3241 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     3301 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     3361 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3421 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3481 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3541 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     3601 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3661 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     3721 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccgatc caacacttac
     3781 gtttgcaacg tccaagagca aatagaccac gaacgccgga aggttgccgc agcgtgtgga
     3841 ttgcgtctca attctctctt gcaggaatgc aatgatgaat atgatactga ctatgaaact
     3901 ttgagggaat actgcctagc accgtcacct cataacgtgc atcatgcatg ccctgacaac
     3961 atggaacatc gctatttttc tgaagaatta tgctcgttgg aggatgtcgc ggcaattgca
     4021 gctattgcca aaatcgaaat acccctcacg catgcattca tcaatattat tcatgcgggg
     4081 aaaggcaaga ttaatccaac tggcaaatca tccagcgtga ttggtaactt cagttccagc
     4141 gacttgattc gttttggtgc tacccacgtt ttcaataagg acgagatggt ggagtaaaga
     4201 aggagtgcgt cgaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc
     4261 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     4321 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     4381 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     4441 cgcgcgcggt gtcatctatg ttactagatc gaccggcatg caagctgata attcaattcg
     4501 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     4561 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     4621 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     4681 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     4741 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     4801 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc
     4861 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     4921 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     4981 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     5041 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     5101 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     5161 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     5221 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     5281 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     5341 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     5401 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     5461 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     5521 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     5581 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     5641 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     5701 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     5761 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     5821 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     5881 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
     5941 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     6001 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     6061 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6781 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6841 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6901 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6961 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7021 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     7081 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7141 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     7201 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     7261 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     7321 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     7381 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     7441 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     7501 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     7561 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     7621 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     7681 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     7741 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     7801 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     7861 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     7921 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     7981 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     8041 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     8101 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     8161 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     8221 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     8281 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     8341 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     8401 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     8461 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     8521 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     8581 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     8641 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     8701 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
     8761 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
     8821 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
     8881 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
     8941 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
     9001 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     9061 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     9121 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     9181 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     9241 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
     9301 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
     9361 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
     9421 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
     9481 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
     9541 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
     9601 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
     9661 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
     9721 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
     9781 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
     9841 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
     9901 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
     9961 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    10021 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    10081 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    10141 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    10201 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    10261 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    10321 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    10381 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    10441 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    10501 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    10561 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    10621 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    10681 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    10741 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
    10801 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
    10861 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
    10921 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
    10981 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca
    11041 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
    11101 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
    11161 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct agccaatacg
    11221 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    11281 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    11341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    11401 acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
//
