LOCUS       sequence-447331-       12224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT4G28430.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12224)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT4G28430-mKOk
FEATURES             Location/Qualifiers
     source          1..12224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2071..2095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3076..3094)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3077..3094)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3123..3132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3162..3181)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3163..3181)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3188..3205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3188..3204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3197..3219)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3362..3545
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3546..3587,3588..3596,3597..4367)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3627..3646)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4237..4256
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4992..5244
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5337..5361
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5882..6673
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6919..7507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7408..7427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7661..7678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7693..7833
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7759..7778)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7919..7941
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8177..8371
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8437..9510)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9939..10568)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11899..11923
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12088..12109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12124..12129,12130..12147,12148..12154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12162..12178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12167..12189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12186..12202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12186..12202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggactctact accacacctc
      721 cttctctccg ttcgaatact agatccgctt tgcgtctagc tcgaaacaac aaaactctcg
      781 taaaatctca cattccattt ctcgatttgg ttttattgtc tccgaagaac aacaacggta
      841 ctccttaccc atcacctgtt tctctcagtt caccatcttc tcctgttact cttcgtgaga
      901 ttctcctttt atctccttcc cctcttcgta aatcgagaac ccgtttaagc aatcggttcg
      961 atatggaagc tgcggaggct gctgtgacgg cacgacggag taagacgaag ggagggcaaa
     1021 atggtctttt ggcctcgccg tcgccgagga atttccggag gtcgagacta aggtcggagg
     1081 cgatggtaga tacaaaggaa aatacggagc caattgttgt cgtgaccgat gaaaagaaac
     1141 aaaaccagag gaaacagaag aagttgggtc gatctaagaa ggagaagcac agttctgttc
     1201 ctctgttagc ttctcctagt ccatcttcag atcaacctca agatgtttgt caaggtgatt
     1261 tagagcggat tagagagaat ataagcgatt tgattatgtg gagagatgtt gcgaaatcaa
     1321 cactttggtt tggttttggc tgtatatgtt tcctatctac ttgctttgct gctaaaggat
     1381 ttaactttag tgttttctca gcgatctcct atcttggact tttgtttctt ggggtatcgt
     1441 tcttgtcaaa tactcttcgc caaagggtta ctgaagaagc gcggagagag cttaaattga
     1501 gtgaagatga tgtattacga atagctagac gaatgcttcc catcaccaat ttagcaattt
     1561 caaagacgag tgagcttttc tctggtgaac cagcaatgac actcaaagtg gcaccttttg
     1621 ttctaatggg agctgagtat ggttacctca taacattgtg gagattatgt gcttttggtt
     1681 tcttccttag cttcactatt ccaaagctat attcatgtta cgcaagtcag cttaaccaaa
     1741 aagttgagtg tgcacaaagg agatttgttg aagcttgggg agtttgcaca cacaagaaat
     1801 tcgtagcagg ctctgcagtt actgcattct ggaatctaac ttctctcaag acccgtttta
     1861 ttgcagtgtt tatcatagta gtagtaatca gatacagacg acagaatcta cagttagatt
     1921 ccgaggatga ggaggagaag aagcaacaag aaaaaactca cccggagcaa caaaaatcgc
     1981 cggaggataa gtcaacttca ccgcggtcag cagaagaaga gcaagcgttg gtgctagttg
     2041 cagaaaccaa agcaccaaag aagctttatc acccagcttt cttgtacaaa gtggtgatgg
     2101 gacgtccgcg gagatctacg cgtgtcgact cgagatatcc aactagtatg gtgagcgtta
     2161 tcaagcctga gatgaagatg aggtactaca tggacggaag cgtgaacgga cacgagttca
     2221 ctattgaagg cgaaggtact ggaaggcctt acgagggaca tcaagagatg actctcagag
     2281 tgaccatggc tgaaggtgga cctatgcctt tcgctttcga tctcgtgtct cacgtgttct
     2341 gctacggaca cagagtgttc actaagtacc ctgaagagat cccggactac ttcaagcaag
     2401 ctttccctga gggactctct tgggagagat ctttggaatt cgaggacggt ggatccgctt
     2461 ctgtgtctgc tcatatctct ctcaggggca acaccttcta ccacaagagc aagtttaccg
     2521 gggtgaactt ccctgctgat ggaccgatca tgcagaacca gtctgttgat tgggagccgt
     2581 ctactgagaa gatcaccgct tctgatggtg tgctcaaggg tgatgtgacc atgtacctta
     2641 agctcgaagg tggtggaaac cacaagtgcc agttcaagac tacctacaag gccgccaaag
     2701 aaatcctcga gatgcctggt gatcactaca tcggacatag gctcgtgaga aagaccgagg
     2761 gaaacatcac tgagcaggtt gaggatgctg tggctcattc tcaattgtta taagcggcca
     2821 tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct
     2881 ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct
     2941 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     3001 aataaaattt ctaattccta aaaccaaaat ccagtgacct gcaggcatgc aagctagctt
     3061 actagtgatg catattctat agtgtcacct aaatctgcgg ccgcactagt gatatcccgc
     3121 ggccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt
     3181 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3241 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3301 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa
     3361 tgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     3421 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     3481 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
     3541 ctgcaatggc aattacctta tccgcaactt ctttacctat ttccgcccgg atccgggcag
     3601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4381 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4441 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4501 ccagcgcggg gatctcatgc tggagttctt cgcccacccc gatccaacac ttacgtttgc
     4561 aacgtccaag agcaaataga ccacgaacgc cggaaggttg ccgcagcgtg tggattgcgt
     4621 ctcaattctc tcttgcagga atgcaatgat gaatatgata ctgactatga aactttgagg
     4681 gaatactgcc tagcaccgtc acctcataac gtgcatcatg catgccctga caacatggaa
     4741 catcgctatt tttctgaaga attatgctcg ttggaggatg tcgcggcaat tgcagctatt
     4801 gccaaaatcg aaatacccct cacgcatgca ttcatcaata ttattcatgc ggggaaaggc
     4861 aagattaatc caactggcaa atcatccagc gtgattggta acttcagttc cagcgacttg
     4921 attcgttttg gtgctaccca cgttttcaat aaggacgaga tggtggagta aagaaggagt
     4981 gcgtcgaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     5041 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     5101 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     5161 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     5221 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
     5281 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     5341 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     5401 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     5461 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     5521 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     5581 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     5641 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     5701 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     5761 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     5821 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     5881 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5941 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     6001 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     6061 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     6121 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     6181 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     6241 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     6301 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     6361 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     6421 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     6481 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     6541 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     6601 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     6661 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     6721 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     6781 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     6841 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7021 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7561 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7681 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7741 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     7801 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     7861 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7921 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     7981 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     8041 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     8101 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     8161 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     8221 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     8281 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     8341 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     8401 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     8461 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     8521 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     8581 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     8641 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     8701 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     8761 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     8821 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     8881 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     8941 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     9001 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     9061 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     9121 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     9181 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     9241 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     9301 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     9361 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     9421 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     9481 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     9541 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     9601 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     9661 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
     9721 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
     9781 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
     9841 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
     9901 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
     9961 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    10021 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    10081 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    10141 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    10201 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    10261 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    10321 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    10381 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    10441 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    10501 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    10561 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    10621 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    10681 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    10741 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    10801 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    10861 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    10921 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    10981 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    11041 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    11101 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    11161 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    11221 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    11281 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    11341 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    11401 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    11461 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    11521 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    11581 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    11641 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    11701 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag
    11761 tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    11821 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
    11881 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
    11941 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagccaa tacgcaaacc
    12001 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    12061 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    12121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    12181 tcacacagga aacagctatg acatgattac gaattcgagc tcgg
//
