LOCUS       sequence-447334-       12068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G55530.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G55530-mKOk
FEATURES             Location/Qualifiers
     source          1..12068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1915..1939)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2920..2938)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2921..2938)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2967..2976
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3006..3025)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3007..3025)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3032..3049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3032..3048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3041..3063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3206..3389
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3390..3431,3432..3440,3441..4211)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3471..3490)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4081..4100
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4836..5088
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5181..5205
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5726..6517
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6763..7351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7252..7271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7537..7677
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7603..7622)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7763..7785
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8021..8215
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8281..9354)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9783..10412)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11743..11767
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11932..11953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11968..11973,11974..11991,11992..11998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12006..12022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12011..12033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12030..12046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12030..12046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggactcccca caatctgttg
      721 tttcaccatt caagattggt gagtcagaga atgagaactc aaattctgtg cagagttctg
      781 gaaaccaatc gaatggcata aattccaatg ggaaagattc taaaagttgt ggccggcaag
      841 accttgttgg tgctcttgag gtttatgtcc atcaggcaag ggatatccat aacatttgta
      901 tatatcacaa gcaagacgtt tatgccaagc tttgccttac aagtgatcct gataagtctg
      961 tctcgaccaa aatcatcaat ggcggtggga ggaatccggt ttttgatgac aatgttaagc
     1021 ttgatgtcag ggttctggac acttccctca aatgtgagat ttacatgatg agcagggtga
     1081 agaattatct tgaggaccag ctgcttggtt ttactctggt tcctatgtcg gaactgctct
     1141 tcaagaatgg gaaactcgag aaagagttct ctctttcttc cactgatctc taccattcgc
     1201 cagctggttt tgttcaattg tcactctcct attatggatc ctaccctgac gtgatggcta
     1261 ttccctctat gccttcgtct gtttctattg atgaaactac caaggatcca gaagggtctg
     1321 aatcagttcc gggtgagttg gataagatag agtttccaga tcctaatgtt gctaatgaaa
     1381 atgagaagat ggtttccgag tattttggga tctcttgttc cactattgac tcagaaactt
     1441 ctgacagctt ggttacttcc gatgctgaga atcatgtgac taattctgtc acttctatac
     1501 tgaagcaaga ttcacctgag agcagcaatg caacaaatgg agctgcttct cctcacgctt
     1561 cagctcactc cgccacagaa acaccaaacc atgaacatct ctctgtcgtg aactccaaag
     1621 cgagttccca ggagtcagag agcgaggctt ctggtgagac atctgaggag aagactgtga
     1681 aatccgtcct cactgtgaag gttgagccag agtcaaaggt ggtgcagcag gatatagtcg
     1741 acatgtacat gaaaagcatg caacagttta cagattcact ggctaagatg aagcttcctc
     1801 tggacattga cagcccaaca aaatcagaaa attcaagctc tgattctcag aagctgccaa
     1861 caccaaagag caacaacgga tcccgtgtgt tctatgggag caggcctttc ttccacccag
     1921 ctttcttgta caaagtggtg atgggacgtc cgcggagatc tacgcgtgtc gactcgagat
     1981 atccaactag tatggtgagc gttatcaagc ctgagatgaa gatgaggtac tacatggacg
     2041 gaagcgtgaa cggacacgag ttcactattg aaggcgaagg tactggaagg ccttacgagg
     2101 gacatcaaga gatgactctc agagtgacca tggctgaagg tggacctatg cctttcgctt
     2161 tcgatctcgt gtctcacgtg ttctgctacg gacacagagt gttcactaag taccctgaag
     2221 agatcccgga ctacttcaag caagctttcc ctgagggact ctcttgggag agatctttgg
     2281 aattcgagga cggtggatcc gcttctgtgt ctgctcatat ctctctcagg ggcaacacct
     2341 tctaccacaa gagcaagttt accggggtga acttccctgc tgatggaccg atcatgcaga
     2401 accagtctgt tgattgggag ccgtctactg agaagatcac cgcttctgat ggtgtgctca
     2461 agggtgatgt gaccatgtac cttaagctcg aaggtggtgg aaaccacaag tgccagttca
     2521 agactaccta caaggccgcc aaagaaatcc tcgagatgcc tggtgatcac tacatcggac
     2581 ataggctcgt gagaaagacc gagggaaaca tcactgagca ggttgaggat gctgtggctc
     2641 attctcaatt gttataagcg gccatgctag agtccgcaaa aatcaccagt ctctctctac
     2701 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     2761 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     2821 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     2881 acctgcaggc atgcaagcta gcttactagt gatgcatatt ctatagtgtc acctaaatct
     2941 gcggccgcac tagtgatatc ccgcggccat ggcggccggg agcatgcgac gtcgggccca
     3001 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     3061 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3121 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3181 gcgaatggaa attgtaagcg ttaatgggtt tctggagttt aatgagctaa gcacatacgt
     3241 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
     3301 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
     3361 atattcactc tcaatccaaa taatctgcaa tggcaattac cttatccgca acttctttac
     3421 ctatttccgc ccggatccgg gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3481 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3541 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     3601 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3661 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3721 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3781 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3841 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3901 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     3961 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4021 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4081 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4141 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4201 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4261 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4321 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4381 ccccgatcca acacttacgt ttgcaacgtc caagagcaaa tagaccacga acgccggaag
     4441 gttgccgcag cgtgtggatt gcgtctcaat tctctcttgc aggaatgcaa tgatgaatat
     4501 gatactgact atgaaacttt gagggaatac tgcctagcac cgtcacctca taacgtgcat
     4561 catgcatgcc ctgacaacat ggaacatcgc tatttttctg aagaattatg ctcgttggag
     4621 gatgtcgcgg caattgcagc tattgccaaa atcgaaatac ccctcacgca tgcattcatc
     4681 aatattattc atgcggggaa aggcaagatt aatccaactg gcaaatcatc cagcgtgatt
     4741 ggtaacttca gttccagcga cttgattcgt tttggtgcta cccacgtttt caataaggac
     4801 gagatggtgg agtaaagaag gagtgcgtcg aagcagatcg ttcaaacatt tggcaataaa
     4861 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     4921 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     4981 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     5041 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga ccggcatgca
     5101 agctgataat tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     5161 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     5221 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     5281 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     5341 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     5401 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     5461 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     5521 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     5581 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     5641 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     5701 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     5761 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     5821 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     5881 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     5941 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     6001 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     6061 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     6121 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     6181 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     6241 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     6301 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     6361 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     6421 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     6481 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     6541 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     6601 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     6661 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     6721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6841 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6901 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6961 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7021 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7081 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7141 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7201 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7261 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7321 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7381 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7441 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7501 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     7561 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     7621 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     7681 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7741 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     7801 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     7861 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     7921 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     7981 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     8041 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     8101 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     8161 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     8221 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     8281 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     8341 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     8401 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     8461 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     8521 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     8581 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     8641 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     8701 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     8761 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     8821 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     8881 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     8941 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     9001 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     9061 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     9121 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     9181 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     9241 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     9301 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     9361 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
     9421 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     9481 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     9541 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     9601 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     9661 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
     9721 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
     9781 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
     9841 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
     9901 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
     9961 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
    10021 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
    10081 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
    10141 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
    10201 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
    10261 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
    10321 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
    10381 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
    10441 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
    10501 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
    10561 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
    10621 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    10681 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    10741 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    10801 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    10861 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    10921 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    10981 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    11041 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
    11101 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
    11161 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
    11221 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
    11281 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
    11341 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
    11401 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
    11461 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
    11521 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag
    11581 gggttggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatcccgagg
    11641 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    11701 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
    11761 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
    11821 gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11881 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11941 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    12001 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
    12061 gagctcgg
//
