LOCUS       sequence-447341-       12286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controls for Bicistronic BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 12286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pBi2FC-C-GRF4-P2A:mTagBFP2
FEATURES             Location/Qualifiers
     source          1..12286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1166..1417
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1354..1375
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1418..1444
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1463..1487
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2310..2334)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2342..2398
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2399..2419
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2420..2440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2441..2461
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3192..3444
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3493..3509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3493..3509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3506..3528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3517..3533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3541..3547,3548..3565,3566..3571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3586..3607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3643..3895)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4046..5071)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5123..5302)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5808..5832
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6353..7144
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7390..7978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7879..7898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8132..8149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8164..8304
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8230..8249)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8390..8412
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8648..8842
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8908..9981)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10410..11039)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gaatggacaa gcagaagaac ggcatcaagg ccaacttcaa
     1201 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     1261 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     1321 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1381 cgccgggatc actctcggca tggacgagct gtacaagtac ccatacgatg ttccagatta
     1441 cgctgggccc aggcctacta gtacaagttt gtacaaaaaa gcaggctccg cggccgcaat
     1501 ggcggcacca ccagcatcat cctcggcgag ggaagagttc gtgtacctcg caaagctcgc
     1561 agagcaagcg gaacgttacg aagaaatggt tgaattcatg gaaaaagtcg ctgaagccgt
     1621 tgacaaagac gaactcaccg tcgaagaacg taatctcctc tccgtcgctt acaaaaacgt
     1681 aatcggcgct cgtcgtgctt cctggagaat catctcttcc attgaacaaa aagaagagag
     1741 tcgtggtaac gatgaccatg tgaccacgat ccgtgattac agaagcaaga tcgaatctga
     1801 gttatcgaaa atctgtgacg gtattcttaa gcttcttgat actagacttg ttcctgcttc
     1861 tgctaatgga gattctaagg ttttttacct taagatgaag ggagattatc ataggtattt
     1921 ggctgagttt aagactggtc aagagaggaa agatgctgct gaacatactc tcaccgctta
     1981 caaagctgct caggatattg ctaatgctga attggctcca acgcatccga ttcgtcttgg
     2041 tttggctttg aatttctctg tgttttacta tgagattctt aattctccag atcgtgcttg
     2101 taatctcgct aagcaggcgt ttgatgaagc cattgctgag ttagatactc ttggagagga
     2161 gtcatacaag gatagtacct tgatcatgca gcttcttcgt gacaatctta ctctctggac
     2221 ttctgacatg caggacgaaa gtccggagga gattaaagaa gcagcagcac caaagcctgc
     2281 tgaagaacag aaggagatca ggcgcgccga cccagctttc ttgtacaaag tggttctcga
     2341 ggcaaccaac tttagcctgc tcaagcaagc aggagatgtt gaggaaaatc ctggcccccc
     2401 taagaaaaag agaaaggttc cgaaaaagaa aagaaaggtg ccaaaaaaaa agagaaaggt
     2461 aatggtgagc aagggcgagg agcttatcaa agagaatatg cacatgaagt tgtatatgga
     2521 gggtactgtg gataaccatc actttaaatg tacatcagaa ggagagggaa agccttacga
     2581 gggtactcaa acaatgagaa ttaaggttgt ggagggtggt cctcttccat ttgctttcga
     2641 tatcttggca acatcttttc tctacggttc aaaaactttc attaaccata cacagggtat
     2701 tccagatttc ttcaagcagt cttttccaga gggattcact tgggaaagag ttactactta
     2761 tgaggatggt ggtgttctta ctgcaactca agatacatct cttcaggatg gatgcttgat
     2821 ctataacgtt aagatcagag gagttaattt cacttcaaac ggacctgtta tgcaaaagaa
     2881 aacattgggt tgggaggctt ttactgagac attgtaccca gcagatggag gtttggaagg
     2941 aagaaacgat atggctctta aattggttgg aggttctcat ctcatcgcaa atgctaaaac
     3001 tacatataga tcaaagaaac ctgctaagaa cctcaaaatg ccaggagttt attacgtgga
     3061 ttacagactt gaaagaatta aagaggcaaa caacgaaact tatgttgaac agcatgaggt
     3121 tgctgtggca agatactgtg atcttccttc taaactgggg cacaaactta attaagagct
     3181 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     3241 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     3301 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     3361 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     3421 cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattggt tccggaacca
     3481 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3541 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3601 cacattaggc tgaattaggc gcgcctattt ctattaattc ccgatctagt aacatagatg
     3661 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
     3721 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
     3781 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
     3841 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga
     3901 aattcggggg atcgatcccg cgaactgtgg acgagaactg tgccaccaag cgtaaggccg
     3961 ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc
     4021 agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg ctggggcgtc
     4081 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     4141 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     4201 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     4261 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
     4321 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     4381 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     4441 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     4501 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     4561 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     4621 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     4681 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
     4741 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     4801 tgcaataggt caggctctcg ctgaatgccc caatgtcaag cacttccgga atcgggagcg
     4861 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     4921 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     4981 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     5041 cagacgtcgc ggtgagttca ggctttttca tatcttattg ccccccggga tcagatctgg
     5101 attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg
     5161 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     5221 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt
     5281 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     5341 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc
     5401 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     5461 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
     5521 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
     5581 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     5641 ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg
     5701 aagctgatcc gtcgagatta atagaaataa attcagccta attcggcgtt aattcagtac
     5761 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     5821 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     5881 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     5941 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     6001 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     6061 cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     6121 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     6181 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     6241 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     6301 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga
     6361 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     6421 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     6481 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     6541 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     6601 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     6661 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     6721 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     6781 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     6841 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     6901 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     6961 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     7021 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     7081 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     7141 ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     7201 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     7261 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac
     7321 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7381 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7441 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7501 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7561 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7621 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7681 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7741 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7801 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7861 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7921 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7981 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8041 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8101 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8161 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     8221 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     8281 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     8341 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     8401 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     8461 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     8521 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     8581 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     8641 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     8701 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     8761 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     8821 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
     8881 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     8941 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     9001 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     9061 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     9121 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     9181 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     9241 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     9301 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     9361 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     9421 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     9481 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     9541 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     9601 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     9661 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     9721 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     9781 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     9841 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     9901 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     9961 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
    10021 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
    10081 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
    10141 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
    10201 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
    10261 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
    10321 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
    10381 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
    10441 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
    10501 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
    10561 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
    10621 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
    10681 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
    10741 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
    10801 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    10861 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    10921 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    10981 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    11041 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    11101 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    11161 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    11221 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    11281 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    11341 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    11401 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    11461 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    11521 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    11581 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    11641 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    11701 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    11761 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    11821 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    11881 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    11941 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    12001 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    12061 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    12121 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    12181 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga
    12241 accctgtggt tggcatgcac atacaaatgg acgaacggat aaacct
//
