LOCUS       sequence-447440-        8789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses precursor peptide and lanthipeptide synthetase from 
            LanII-2B (FAST-RiPPs) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, 
            Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, 
            Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
  TITLE     A scalable platform to discover antimicrobials of ribosomal origin.
  JOURNAL   Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
  PUBMED    36253467
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-17"; volume: "13(1):6135. doi"; pages: " 
            10.1038/s41467-022-33890-w"
COMMENT     Sequence Label: pET28:LanII-2B
FEATURES             Location/Qualifiers
     source          1..8789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3489..3507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3508..3532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3549..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3877..3924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3929..3947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3948..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      7218..7265
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7306..7761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7393..7412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7603..7624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7853..8668)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7853..8668)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8576..8595
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8660..8679
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaatatggta atacgactca ctatagggga attgtgagcg gataacaatt ccaaggagat
     3541 atacaatgca ccaccaccac caccacgcaa tgaagaaaga gtcgattgtc cgtgcgtgga
     3601 aagatccagc gtttcgtgca agtctgtcag cggaagagcg tgccagcctg cctgaaagcc
     3661 ctagcggtcg cgcattgacc gaattggacg aaagcgaatt gctgggtgtt acaggtggaa
     3721 aagccgcttt agaaccgtcg accgggtgca cggatggcgt tccgacttgc gggattgttc
     3781 cattttgtga ccttgaagtt gcgtaaagaa ggtatttgcc cgataatcaa taccccaggc
     3841 ttctaacttt ttccactcgc ttgagccggc tacggtctag cataacccct tggggcctct
     3901 aaacgggtct tgaggggttt tttgccctta atacgactca ctatagggga attgtgagcg
     3961 gataacaatt ccaaggagat atacaatgca attcattgcc ccggtgtttc catggaagaa
     4021 agctgccttt ctgcacgagc gtgcaactgc aaaacatgaa gatccgccac ccgaatgtga
     4081 accggtactc caggaagcag agcgtcgcgc gagcgcgtgg cgtcaactga tggccggaga
     4141 tgatgccaca ctggatgaac gtttgaaaag cgtgggtctt gatcgtgaag catttctccg
     4201 tattctggtc cgcagtggca atcaagagga tgcaccagaa gataatggtc cgtgggtttc
     4261 tctgattgag gaagttttgg aacagcgcca tgctggagaa cctttaccac catctcttca
     4321 accaccgtca ggtccgggac aacctgggtt ggcgttttcc ggattcgttc agcccttctt
     4381 gcgcctggca gcagcacgct tgcgtacggt taccgccgct ttacaggccc gttttggact
     4441 tggtctgccg ttactgagtc ccgaagccga agcgtcactt ctggaagggc tggcacgccg
     4501 cttacaaagt ctgtcaacgc gtactctgat ccttgaattg aatgttgcac gtgtaatgga
     4561 agaattaccg ggcgatacgc cgcaagaacg ttttcatcat ttctccacgg ttcgcctgca
     4621 agaacctcgt gtactggcac ctctgcttga ggaatatccg gtacttgcac gcctgttggc
     4681 gacgtccgcg gaacgttggt tgacagtgag cctggaactc ttagaacgtc tggcttcgga
     4741 acgtgaacaa ttggggcaat cctttctggg cgggcaagat attgggacgc tggtaggctt
     4801 acagagtggt gtgtccgatc ttcatcgtga aggccgtggc gttgtgctgt tacgtttcag
     4861 cagcggactg cgtcttgtgt ataaaccgaa atcgctggcg gtagatgttc gctttcaacg
     4921 tctgttacaa acactgaatg cgtggggagc acgtcatccg catcgcgtac tgacagtatt
     4981 ggatcgcggc acacatggtt gggttgaaca cgttaaagca gaaggctgcg acagtcgtga
     5041 agcacttcaa cgcttttact ggcgtcaagg tagcagcctg gccttgttgc atctgttagc
     5101 agcagtagat tttcatttgg aaaatctgat tgcagcaggt gaatatccgg tgctggtaga
     5161 tttagaggcc ctcttccatc agcgtctcgc actggaaacg ggtgatagcg ccatgcgccg
     5221 tgcctggggt gaacttgatc gttctattat ttcagttggt atgttgccga tgctgatttt
     5281 cgggcgtgcc ggccgtgccg gtgttgatat gagcggactc ggcggtgaag cgggtcaacg
     5341 ttctccgcat gcggttccta tggtcgaaga tgcccattcg gatactatgc gcatggtacg
     5401 tcgtcaagga cgtacctctg gttccagcaa ccgtcctctg ctgcagggcc agccggtcga
     5461 tccagcagga tttactgaag atattgtaca gggttttgaa gagacgcatc gcctgttacg
     5521 tcagcatcgt gaagaacttg gtgcgcgcct gcagtcattt gcagatgttg aagttcgtca
     5581 tatcgtacgt gcaacgcagc gttacgcgct tctgttgcaa gaaagcgtgc atccagattt
     5641 cctgcgcgaa ggccttgaac gtgataaagt gttagataac ctgtgggctg aagcgtcgct
     5701 tgttccggct ctgcgccgcc tcgtacctag tgaacatgct gatctgcgtt taggagatat
     5761 ccctctcttc acaacacgcc cgggtcagcg tcacctctgg aactccaccg gggaatgtat
     5821 ccgtgactac tttgcacgtg actcgctgtc ggaagtacgt gaacgtttag ctcgcatgga
     5881 tgaaaaggat tgtgattggc aagtctcact gatccgcaaa tccatggtgt ctcttgacaa
     5941 ggggcgctct acggcgcgtc cggcagcacg tactaccccg ccgccatcat tgccgccggc
     6001 ctcacgtgag gattgtctgg ctgcagcagt ttcgattggc gagcaattag cggcgcgcgc
     6061 cattcgtggt aaaacggatg tatcatggat cggcatgtcg ctggaagatc tgggtcagtg
     6121 gcgctggtcg ttaagtcctt taggtacgga cctttacgaa ggtgtaggcg gtgtgtccct
     6181 ctttctcgcg tacctggcac gtgaaacagg gcgtgcggat tttgaacaac tggcccgtgc
     6241 aggccttgaa actgtgcgcg atgcttggcg caatcctgat cccagcgatg caggtgtggg
     6301 cgcctatgtc ggtcgtacaa gttccgcgta cgtgctggca cacttgtccg cactgtggaa
     6361 tcaaccagca cttttggacg aagtgattgc aggcctgccg acgttggaac cgctgattga
     6421 tgcggataca cgcctcgatt tattaagcgg tagcgccggc tgtgcactgg tgctgttggg
     6481 actccatgcc cgtaccggtg atcctcgttt actggatgct gctcgtcgtt gcggtgaacg
     6541 cttgctggtc actgcccagc cgtgtcctga aggtggtgta ggctggaaag gtccggcagc
     6601 atggcgcccg ctttccggtt ttagtcatgg tgccgcagga attgcatatg cactgctggc
     6661 cctcgcagcc gcaaccgggg acacgcgcta tcgcgaattg gcacatcaag cacttatcca
     6721 tgaacgcgcg ctgttcgtgc cagaacgtgg taattggaaa gacttacgtg agccggaaac
     6781 ctcgacgggt gaagcgagct cgggatttat ggtcacctgg tgtcatggtg caccaggcat
     6841 tgccctggga cgcttgtgtt cacttcgcta tttggatggt cctgaagttc gtgcagaatt
     6901 agaaacagcc cttacaacaa cgttaagcga aggttttggc ggtggtcatt gcctgtgcca
     6961 tggtgatctg ggcaacgtcg aaagtctctt actggcggga gaagttttag gtgatgagcg
     7021 ttggtcacat gcagcacgcg agcgtgcagc gcattcactc catcaggcac gtactcgcgg
     7081 ttggttatgc ggtctgccgc gcggtacaga gacgcccggt ctgcttatgg gactcgcggg
     7141 aattggttat ggtttattac gtctggccgc tcctgaacgc gtcccaccag tattaacgct
     7201 ggccatgccg taacggtcta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     7261 ttttgagcgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
     7321 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7381 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7441 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7501 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7561 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7621 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7681 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7741 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa
     7861 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     7921 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     7981 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     8041 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     8101 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     8161 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     8221 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     8281 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     8341 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     8401 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     8461 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     8521 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     8581 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc
     8641 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     8701 acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8761 gaccccgtag aaaagatcaa aggatcttc
//
