LOCUS       sequence-44773-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F2-GTT-F3-GGC
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..36
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             37..102
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 actgccacca tggctccaaa gaagaagcgt aaggtagact acaaagacca tgacggtgat
       61 tataaagatc atgacatcga ttacaaggat gacgatgaca agggtacccg cccatatgct
      121 tgccctgtcg agtcctgtga ccgccgcttc agccgtagcg acaacctctt ggagcacatc
      181 cggattcaca caggccagaa gcccttccag tgtcgcatct gcatgaggaa cttcagcttt
      241 aagtcgtctc tcacccgcca catccgcacc cacacaggcg agaagccttt tgcatgcgac
      301 atctgcggca ggaagttcgc ggaccgtagc cacctcaccc gccacaccaa gatccacaca
      361 ggtggatccc aactagtcaa aagtgaactg gaggagaaga aatctgaact tcgtcataaa
      421 ttgaaatatg tgcctcatga atatattgaa ttaattgaaa ttgccagaaa ttccactcag
      481 gatagaattc ttgaaatgaa ggtaatggaa ttttttatga aagtttatgg atatagaggt
      541 aaacatttgg gtggatcaag gaaaccggac ggagcaattt atactgtcgg atctcctatt
      601 gattacggtg tgatcgtgga tactaaagct tatagcggag gttataatct gccaattggc
      661 caagcagatg aaatgcaaga ctatgtcgaa gaaaatcaaa cacgaaacaa acatatcaac
      721 cctaatgaat ggtggaaagt ctatccatct tctgtaacgg aatttaagtt tttatttgtg
      781 agtggtcact ttaaaggaaa ctacaaagct cagcttacac gattaaatca tatcactaat
      841 tgtaatggag ctgttcttag tgtagaagag cttttaattg gtggagaaat g
//
