LOCUS       sequence-44774-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F3-GCT
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..33
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             34..99
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tgccccatgg ctccaaagaa gaagcgtaag gtagactaca aagaccatga cggtgattat
       61 aaagatcatg acatcgatta caaggatgac gatgacaagg gtacccgccc atatgcttgc
      121 cctgtcgagt cctgtgaccg ccgcttcagt cagtctggtg acctgacccg tcacatccgg
      181 attcacacag gccagaagcc cttccagtgt cgcatctgca tgaggaactt cagccagtct
      241 ggtgacctca cccgccacat ccgcacccac acaggcgaga agccttttgc atgcgacatc
      301 tgcggcagga agttcgcgca ccggcagagc ctgacacggc acaccaagat ccacacaggt
      361 ggatcccaac tagtcaaaag tgaactggag gagaagaaat ctgaacttcg tcataaattg
      421 aaatatgtgc ctcatgaata tattgaatta attgaaattg ccagaaattc cactcaggat
      481 agaattcttg aaatgaaggt aatggaattt tttatgaaag tttatggata tagaggtaaa
      541 catttgggtg gatcaaggaa accggacgga gcaatttata ctgtcggatc tcctattgat
      601 tacggtgtga tcgtggatac taaagcttat agcggaggtt ataatctgcc aattggccaa
      661 gcagatgaaa tgcaagacta tgtcgaagaa aatcaaacac gaaacaaaca tatcaaccct
      721 aatgaatggt ggaaagtcta tccatcttct gtaacggaat ttaagttttt atttgtgagt
      781 ggtcacttta aaggaaacta caaagctcag cttacacgat taaatcatat cactaattgt
      841 aatggagctg ttcttagtgt agaaagagct tttaattggt ggagaaatga ttaa
//
