LOCUS       sequence-447797-        3840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows for expression of recombinant antibodies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Institute for Protein Innovation (IPI)
  TITLE     IPI empty backbones for antibody production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTipi2.1
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..70)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(67..270)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(96..116)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     748..770
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(808..826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        894..998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(999..1067,1068..1859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1217..1236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2030..2618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2519..2538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2772..2789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2906..2954
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2979..2998
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     misc_feature    3020..3608
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3126..3137
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3535..3555
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(3677..3696)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          3679..3811
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
       61 taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      121 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      181 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      241 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      301 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      361 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      421 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      481 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      541 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      601 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc tagttaagcc
      661 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      721 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      781 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      841 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      901 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      961 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1021 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1081 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1141 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1201 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1261 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1321 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1381 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1441 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1501 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1561 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1621 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1681 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1741 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1801 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1861 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1921 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2641 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2701 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2761 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2821 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2881 aagcgggcag tgagcgcaac gcaatgaaca aacgacccaa cacccgtgcg ttttattctg
     2941 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     3001 tagcctcccc cgtttccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     3061 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
     3121 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
     3181 acacaggcga gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca
     3241 gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
     3301 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
     3361 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
     3421 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
     3481 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
     3541 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
     3601 ggttgattgt cgagatatca gagctcgcgg ccgctagcaa gcttctcgag ggtacctcta
     3661 gagaattcac tagtcgacct gtggagagaa aggcaaagtg gatgtcagta agaccaatag
     3721 gtgcctatca gaaacgcaag agtcttctct gtctcgacaa gcccagtttc tattggtctc
     3781 cttaaacctg tcttgtaacc ttgatactta cctgcaggtc cggaggctgg atcggtcccg
//
