LOCUS       sequence-448011-       14968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express RUBY transgene under the control of desired promoter 
            (Note: R2 Gateway R1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14968)
  TITLE     Siddiqi August 2024 Plasmids
REFERENCE   2  (bases 1 to 14968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGENT R2 GW R1 RUBY
FEATURES             Location/Qualifiers
     source          1..14968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    461..585
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(626..931)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(728..747)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1273..1932)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1847..1866
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1986..1992,1993..2010,2011..2016))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2041..2165)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2178..6128
                     /label=RUBY
     CDS             3678..3734
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4569..4625
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6725..6742)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6725..6741)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6734..6756)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6970..7646
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     7583..7603
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             7709..8242
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     polyA_signal    8299..8473
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8551..8575
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9096..9887
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10133..10721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10622..10641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10875..10892
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10907..11047
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10973..10992)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11133..11155
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11391..11585
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11651..12724)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13153..13782)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctgctct agagggcccg acgtcgcatg cctgcaggtc actggatttt
      241 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
      301 gggtttctta tatgctcaac acatgagcga aaccctataa gaaccctaat tcccttatct
      361 gggaactact cacacattat tctggagaaa aatagagaga gatagatttg tagagagaga
      421 ctggtgattt ttgcggactc tagcatggcc gcgggatatc accactttgt acaagaaagc
      481 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      541 acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact
      601 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
      661 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
      721 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
      781 aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
      841 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
      901 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
      961 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
     1021 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
     1081 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
     1141 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
     1201 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
     1261 ggatccacgc gtttacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1321 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1381 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1441 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1501 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1561 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1621 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1681 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     1741 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1801 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1861 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1921 ttttttctcc attttagctt ccttagctcc tgaaaatctc gacggatcct aactcaaaat
     1981 ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgcggccgc
     2041 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     2101 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa
     2161 cttgtgatat cactagtatg gatcatgcga ccctcgccat gatcctcgcg atctggttca
     2221 tcagcttcca cttcatcaag ctgctgttct cccagcagac caccaagctg cttccgccag
     2281 gaccaaagcc gcttccgatc atcggcaaca tccttgaggt gggcaagaag ccgcatcggt
     2341 ccttcgccaa cctcgccaag attcacggcc cactcatttc cctcagactc ggctctgtga
     2401 ccaccatcgt tgtgtcctct gccgacgtgg ccaaagagat gttcctcaag aaggatcacc
     2461 cgctctccaa ccgcacgatc ccgaatagtg ttacagccgg cgaccaccac aagctcacca
     2521 tgtcttggct cccggtgtct ccgaagtggc gcaacttccg caagattacc gccgtgcatc
     2581 tgctctcccc acagagactc gatgcctgcc agacattcag gcacgccaag gtgcagcagc
     2641 tctacgagta cgttcaagag tgcgcccaga aaggccaggc cgtggatatt ggcaaggccg
     2701 cctttacgac cagcctcaac ctcctcagca agctgttctt cagcgtcgag ctggcgcacc
     2761 acaagtccca taccagccaa gagttcaaag agctgatctg gaacatcatg gaagatatag
     2821 gcaagccgaa ctacgccgac tacttcccga ttctcggctg cgttgaccca tctggcatta
     2881 gaagaaggct cgcctgctcc ttcgacaagc tgatcgccgt gttccagggc atcatctgcg
     2941 agagactcgc cccagattcc tccaccacaa ctaccaccac caccgacgac gtgctcgatg
     3001 tgctcctcca gctgttcaag cagaacgagc tgacgatggg cgagatcaac cacctcctcg
     3061 tggacatctt cgacgccggc accgatacca catcctccac attcgagtgg gtgatgaccg
     3121 agctgatccg caatccagag atgatggaaa aggcccaaga ggaaatcaag caggtcctcg
     3181 gcaaggacaa gcagatccaa gagtccgaca tcatcaacct gccgtacctc caggcgatca
     3241 tcaaagagac actccgcctc catccgccga ccgtgttctt gctcccaaga aaggccgaca
     3301 ccgatgtcga gctgtacggc tacatcgtgc cgaaggatgc ccagatcctc gtgaacctct
     3361 gggccattgg cagggaccca aacgcctggc agaacgccga tattttcagc ccagagcgct
     3421 tcatcggctg cgagatcgat gttaagggcc gcgatttcgg cctccttcca tttggcgctg
     3481 gccgcagaat ttgcccaggc atgaatctcg ccatcaggat gctcaccctc atgctcgcca
     3541 cactcctcca gttcttcaac tggaagctcg aaggcgacat ctccccgaag gacctcgaca
     3601 tggacgagaa gttcggcatt gcgctccaaa agaccaagcc gctcaagctc atcccgattc
     3661 cgcgctacgg tagcggagct accaatttta gcctccttaa gcaggcaggt gatgtagaag
     3721 agaaccccgg gcctatgaag atgatgaacg gcgaggacgc caacgaccag atgatcaaag
     3781 agtccttctt catcacccac ggcaacccga tcctcaccgt cgaggataca catccgctca
     3841 ggccgttctt cgagacatgg cgcgagaaga ttttctccaa gaagccgaag gccatcctca
     3901 tcatctccgg ccactgggag acagtgaagc caaccgtgaa cgccgtgcac atcaacgaca
     3961 ccatccacga cttcgacgac tacccagccg ccatgtacca gttcaagtac ccagctccag
     4021 gcgagccaga gcttgcgaga aaggtggaag agatcctcaa gaagtccggg ttcgagacag
     4081 ccgagacaga ccaaaagagg ggccttgatc acggcgcctg ggttccactc atgctcatgt
     4141 atccagaggc ggacatcccg gtgtgccagc tctcagttca gccacatctc gacggcacct
     4201 accactacaa tctcggcaga gccctcgcgc cgctcaagaa tgatggcgtg ctcattattg
     4261 gctccggcag cgccacacat ccactcgatg agacaccgca ctacttcgat ggtgttgccc
     4321 cttgggccgc tgccttcgat tcttggctta ggaaggccct catcaacggc cgcttcgagg
     4381 aagtgaacat ctacgagagc aaggccccga actggaagct cgcccatcca tttccagagc
     4441 acttctaccc gctccacgtt gtgctcggcg ctgctggtga aaagtggaag gccgagctga
     4501 tccactcctc ctgggatcat ggcacacttt gccacggctc ctacaagttc acctccgccg
     4561 gatccggagc aaccaacttt agcctgctca agcaagcagg agatgttgag gaaaatcctg
     4621 gccccatgac cgccatcaag atgaacacca acggcgaggg cgagacacag cacatcctca
     4681 tgatcccgtt catggcgcag ggccacctca ggccatttct cgaactcgcc atgttcctct
     4741 acaagcgctc ccacgtgatc atcaccctgc tcacaactcc gctcaatgcc ggcttcctca
     4801 ggcacctcct tcaccaccat tcctactcct ccagcggcat caggatcgtc gagctgccat
     4861 tcaactccac caaccacgga ctcccaccgg gcatcgagaa caccgataag ctcacactcc
     4921 cgctcgtggt gtccctcttc cattccacca tcagcctcga tccgcacctc cgcgattaca
     4981 tctccaggca tttcagccca gccaggccac cactctgcgt gatccatgat gtgttcctcg
     5041 gctgggttga ccaggtggcc aaggatgtgg gctctacagg cgtggtgttc acaacaggcg
     5101 gcgcttatgg cacatccgcc tacgtgtcca tctggaacga tctcccgcac cagaactact
     5161 ccgacgacca agagttcccg ctgccaggct tcccagagaa ccataagttc cgcaggtccc
     5221 agctccatcg gttcctcaga tatgccgacg gctccgacga ttggtccaag tatttccagc
     5281 cgcagctccg ccagtccatg aagtcttttg gctggctctg caactccgtg gaagagatcg
     5341 agacactcgg cttctccatc ctccgcaact acaccaagct gccgatctgg ggcatcggcc
     5401 cacttattgc ttccccagtg cagcactcct cctccgacaa caattcaaca ggcgccgagt
     5461 tcgtgcagtg gctcagcctc aaagagccgg actccgtcct ctacatctcc ttcggctccc
     5521 agaacacgat cagcccgacg cagatgatgg aactcgctgc tggccttgag tcctccgaga
     5581 agccattcct ctgggtgatc agagccccgt tcggcttcga catcaacgaa gagatgcgcc
     5641 cagagtggct gccagagggc tttgaggaac gcatgaaggt gaagaaacag ggcaagctcg
     5701 tgtacaagct cggcccgcag cttgagatcc tcaaccatga atccatcggc ggctttctca
     5761 cccactgcgg atggaacagc atccttgagt ctcttcgcga gggcgttccg atgcttggat
     5821 ggccacttgc tgccgagcag gcctacaacc tcaagtacct cgaagatgag atgggcgtcg
     5881 cggttgagct tgctagaggc ctcgaaggcg agatctccaa agagaaggtc aagcgcatcg
     5941 tcgagatgat ccttgagcgc aacgagggct ccaaaggctg ggagatgaag aatcgcgccg
     6001 tggaaatggg caaaaagctc aaggacgccg tgaacgagga aaaagagctg aagggctcct
     6061 ccgtgaaggc gatcgacgat ttcctcgacg ccgtcatgca ggccaaactt gagccaagcc
     6121 tccagtgata gtgaatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaaggtt
     6181 gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct
     6241 aatattaaat gaatgtaaca tctcattata atgaataaac aaatgtttct ataatccatt
     6301 gtgaatgttt tgttggatct cttctccagc atataactac tgtatgtgct atggtatgga
     6361 ctatggaata tgattaaaga taagacctaa ctgagtaagc tagcttgttc gagtattatg
     6421 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
     6481 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
     6541 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
     6601 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
     6661 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac actagatccc caaacaagct
     6721 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6781 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6841 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgccaacat
     6901 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     6961 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     7021 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     7081 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
     7141 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     7201 aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata
     7261 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
     7321 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
     7381 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     7441 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     7501 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     7561 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     7621 catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct
     7681 cgagctttcg cagatctgtc gatcgaccat ggtacgcagc agcaacgatg ttacgcagca
     7741 gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag
     7801 gctcggccct gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt
     7861 cggagacgta gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct
     7921 ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct
     7981 cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct
     8041 cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa
     8101 gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga
     8161 tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat
     8221 cgacccaagt accgccacct aacaattcgt tcaagccgag atcggcttcc cggcctagag
     8281 tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat
     8341 tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     8401 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac
     8461 taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa
     8521 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     8581 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     8641 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     8701 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     8761 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     8821 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     8881 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     8941 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     9001 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     9061 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
     9121 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     9181 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     9241 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     9301 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     9361 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     9421 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     9481 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     9541 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     9601 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     9661 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     9721 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     9781 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     9841 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
     9901 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
     9961 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
    10021 cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg
    10081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10261 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10321 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10381 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10441 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10501 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10561 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10621 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10681 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10741 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10801 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10861 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
    10921 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
    10981 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
    11041 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    11101 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    11161 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
    11221 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
    11281 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
    11341 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
    11401 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
    11461 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
    11521 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
    11581 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
    11641 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
    11701 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
    11761 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
    11821 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
    11881 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
    11941 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
    12001 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
    12061 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
    12121 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
    12181 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    12241 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    12301 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    12361 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    12421 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    12481 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    12541 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    12601 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    12661 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    12721 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
    12781 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    12841 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    12901 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    12961 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    13021 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    13081 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    13141 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    13201 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    13261 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    13321 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    13381 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    13441 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    13501 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    13561 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    13621 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    13681 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    13741 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    13801 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    13861 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    13921 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    13981 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    14041 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    14101 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    14161 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    14221 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    14281 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    14341 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    14401 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    14461 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    14521 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    14581 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    14641 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    14701 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    14761 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    14821 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    14881 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    14941 agcagcggag gggttggatc aaagtact
//
