LOCUS       sequence-44809-e        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST53-CypB-Del4 N-term GFP
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(536..635)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(652..671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(653..671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(676..692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(676..692)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(794..813)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(872..891)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcttatgtcc tacgtcaggt gtattttgac ctacgaattg gagatgaaga tgtaggccgg
       61 gtgatctttg gtctcttcgg aaagactgtt ccaaaaacag tggataattt tgtggcctta
      121 gctacaggag agaaaggatt tggctacaaa aacagcaaat tccatcgtgt aatcaaggac
      181 ttcatgatcc agggcggaga cttcaccagg ggagatggca caggaggaaa gagcatctac
      241 ggtgagcgct tccccgatga gaacttcaaa ctgaagcact acgggcctgg ctgggtgagc
      301 atggccaacg caggcaaaga caccaacggc tcccagttct tcatcacgac agtcaagaca
      361 gcctggctag atggcaagca tgtggtgttt ggcaaagttc tagagggcat ggaggtggtg
      421 cggaaggtgg agagcaccaa gacagacagc cgggataaac ccctgaagga tgtgatcatc
      481 gcagactgcg gcaagatcga ggtggagaag ccctttgcca tcgccaagga gtagaaccca
      541 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
      601 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
      661 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
      721 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
      781 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
      841 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      901 caatcagtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
      961 aacatggcaa ggtagcgttg ctatgatgta cagatgagat gtcagactaa actggctgac
     1021 gattatgctc ttccgacatc aagcatttta tccgtacttc tgatgatgca tggctactcc
     1081 accactgcga tcccgggaaa aacagcatca agtattagaa gaatatctgg atccagtgaa
     1141 atattgtgat gcctggcagg tctggctcgg atgcaatcga atc
//
