LOCUS       sequence-448246-        2279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express gRNA for FXR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Chen X, Fansler MM, Janjos U, Ule J, Mayr C
  TITLE     The FXR1 network acts as signaling scaffold for actomyosin 
            remodeling.
  JOURNAL   Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015.
  PUBMED    37961296
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 5 
            August 2024. doi: 10.1016/j.cell.2024.07.015."
COMMENT     Sequence Label: BPK1520-FXR1-N202S-gRNA
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(184..288)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        395..643
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     395..415
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     566..585
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        664..739
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      complement(843..1431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(923..942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1602..2279,1..114,115..183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2225..2244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
       61 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      301 aaaagtgcca cctgacgtcg ctagctgtac aaaaaagcag gctttaaagg aaccaattca
      361 gtcgactgga tccggtacca aggtcgggca ggaagagggc ctatttccca tgattccttc
      421 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
      481 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
      541 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
      601 cgatttcttg gctttatata tcttgtggaa aggacgaaac acccagaaat gaagaggcca
      661 ctagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      721 agtggcaccg agtcggtgct ttttttaagc ttgggccgct cgaggtacct ctctacatat
      781 gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1261 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1501 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttc
//
