LOCUS       sequence-448441-       11568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express METTL121B gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:METTL121B_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..11568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    4983..5117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(5419..6132,6133..6135,6136..6138))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5422..6132,6133..6135,6136..6138))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5464..5485)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5813..5832
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6072..6093
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7319..7335)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7319..7335)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7390..7408)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7418..7435)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7418..7434)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7427..7449)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7575..8030)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7712..7733)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7924..7943
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8057..8161
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8162..8230,8231..9022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8380..8399)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9193..9781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9682..9701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9935..9952
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10069..10090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10105..10110,10111..10128,10129..10135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10143..10159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10148..10170
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10167..10183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10167..10183
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10202..10222
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10204..10222
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acctgtggtc tgtgtgggtc ctaatgcccc agtatattga tggagactct atactgctcg
       61 gtgagctcca tctttcggat aagatgttaa aacaaggtcc cgactctctg tggtggtaaa
      121 aaattcccag gatgtccttc ctaccctggc atcctggaca aatttgctca ctgtcctctg
      181 tccatcatgg cctcctaacc atccccatat cataattggc tttatcactc tagctggggc
      241 cgaaatcgca tacttccatt ttatgaggta cgctaaaatc agtatgcgag ccaagtaata
      301 tgtccgaata cttccggcga gattctagag tgtgcattcg atgcacgcta cgctattcca
      361 tgatgccccg tgagagaatt cttgaatcgg agagaagcaa cgcaactcat gcgggtaggt
      421 tacgtgacga tgacatattg gcggatgtag tacgtccaag ttccattcat actgctcaca
      481 ttcatatttt atagaatgtt cttttcccac agccaagtag taagtttaaa ttcaaataca
      541 gtacctacta agtagtagta gacggtttcg gacgcagccc ctgtctcctc tccaccaata
      601 tactggtgtg tagtgtgcgg tctggtgcaa tatggctgcc atcgcgtcat ccaggtggat
      661 gctgcacact ggtggttgat gaggagattt tgcccaaaaa agtgttttga gtttccagaa
      721 aagcgcgata taaatgtaag gaattatcat tagcttatta taatggaact ttctggataa
      781 cttgtgccag agctaggggc cattaaaact ttgccctggg gtacctcagg gagctgcaag
      841 gaagtgctat agggctaatg aaatgtttgg gggaaaaaaa aaaattttat aagtaaaaat
      901 aaaactaaaa taattaataa tttcattgtt gaaaattata gtaaaattga taaacaagat
      961 aagcagcatt agtataaaaa actaaaaaca aacgtaattt aaaataatta aattgtttta
     1021 aataaattat acaaaataat gtttacaacg acacggtggt gcagtaggta gtgctgtcgc
     1081 cagcaaagca agaaggtcgc tggttcgagc ctcagctgga tcacttgatg tttctgtgtg
     1141 gagcattcgc gtgcgtttaa ccccagatta ataaagagac taagccgaaa agaaaataaa
     1201 tgtatgaaaa tgtttacgta aaatgtaaat aaatgcatga ttaataaaat atatgaattt
     1261 taaagattga ggtagaattg ttttattatt cacacatttg gttattttta atcagtgaca
     1321 acttgtgatg ctctttatat tgtttattac gctactttga atattcagtc tcaaattgca
     1381 tgtaaatacg acttcaaagc agaattagtt taatttaatt gcctgtaaac agtgttgtac
     1441 tttgatggtg attgtctgat aataatatga tttcactatt taaaatttga aaaaaaaaaa
     1501 tctgaaaaat actcaccggc cactttatta ggtacacctg tctaactgct tgttaacgta
     1561 aatttctaat cagccaatca ccaggcagca actcagtgca tttaggcatg tagacatggt
     1621 caagacgatc tgctgcagtt caaagcgagc atcagaatgg agaagcaagg tgatttaagt
     1681 gactttaaac gtgacgtgtt tgttggtgcc agacgggctg gtctgagtat ttcagaaact
     1741 gctgatttac tggggttttc acacacaaac atctctgggg tttacagaga atggtccaaa
     1801 aaaagataat atccagtgag cggcagttct gtgggggcaa atgtcttgtt gatgccagag
     1861 gtcagaggag aatggcctga ctggttccag ctgatagaaa ggcaacagta attcaaataa
     1921 ccactcgtta gaactgaggc atgcagaaga gcacctttga acacaacatg tcgaaccttg
     1981 aggcgggtgg gctacagcag ctcaagacca catcgggtgc cactcctgtc agctaagaac
     2041 aggaaactga ggctacaatt cgcacaggct caacataagt agacaataga agattgaaca
     2101 ttatgttgcc tggtcagatg gatacattct accttgtatc aacagttcag gctggtgatg
     2161 gtggtgtaat ggtgtggggg atatattctt ggcacacttt gggcccatta gtaccaatta
     2221 accatcgtgt caacgccaca gcctaactga gtattgttgc tgaccatgtt catttctttt
     2281 tgaccacagt gtacccatct tctgatggct acttcctgca ggataatgca ccatgtcata
     2341 aagcgtgaat gatctcagac tggtttcttg aacatgacaa tgagttcact gtgctcgaat
     2401 gacctccaca gtcaccagat ctcaatccaa tagagcgcct ttggggtgtg gtagaatgga
     2461 agattcgcat cctggatgtg cagccgacaa atctgcatga tgctagcatg tcaatatgga
     2521 ccaaaatctc tgaggaattt tccagtacct ggttgaaatg caaaaggaag ggaatctatg
     2581 ctaaggcaaa aagaggtcca acccagtact agtggggtgt acataataaa gtggccggtg
     2641 agtgtatatt gttaaggata tctagataat ccaaataatt tacagcacta tttaaaaaaa
     2701 aaactttatg tcaccaagtc tgcatttatt tgatcacaat tacagtcgaa aaagcaatat
     2761 cgggatacat tgtttccatt taaaataact atattatatt ttaaatattt ttgacaatgt
     2821 tatttcttta atgccaaagc taaattgtat tcaatctgaa ttttagtgtc acatgatcct
     2881 tcagaaaata ttaacacatt gctaaaaatg tatattatta tcaatttgga tttttttttt
     2941 ttttttgtat attaacctat gctattggtt actatacaaa agtattactg tgaatatatg
     3001 catatacaga accgtatcaa gcgcgggggg tggttgggtt atgtccccat ttggaaagac
     3061 ctggaaacat tcataatgac taaaattgca cggaaaaata taatatctat gagtattgct
     3121 gatcagcagc acaaaaccag aactccatat taataaacag ccaatgatgc agtgagtttg
     3181 tgcatgtttt cattcaaact cgtgtgattg tgctcaaaca cctgttgtga gcagcagttg
     3241 caaacagtaa acctcaagag gagccagtca accttcacaa acactcaacc tctggctcac
     3301 tcactaaaca attcgcgtta atatctaaaa taaaaatttc cagcaccaaa cagctctcag
     3361 atttcggagt acactgacca agaggtaaga agtaacgtta cattactagg acgactattg
     3421 taagactaga tatgtaatat ttggaagcgt acgtgtttta agtgacatat agagtagagg
     3481 aactgatgga aatacgattt gcataatgta aaatgatata tattaaaata taggctacag
     3541 taggtaacgt ggtttacata taacgttact gtgttaatgt agtgctcagt tactcgtgtt
     3601 gacagttatt attagtgctt gctaatttgt ttgttaatgc ttctcttgcg tatttgatta
     3661 ccttcaggcg aatctaaaac atatcagatg tagaaacggc agaaaaatgt tgaagaaaat
     3721 acgagaatag tagcttttgg agcacataga aaagtacatc agataaagcc taccgtttaa
     3781 acgcagacgc cgctctcgca ctgaatgctg ggtactgtag tccttatatt gtatgcttaa
     3841 tgtgcgtctc aacgggaaac tagagttagg aaaactacaa tttcctgtgg aaaatgtttc
     3901 tgttttcggg gaaacagcga aagaaagata tggttccgcg tttttttctt actttgaata
     3961 ataaaataag acctaactac acacttcgct tactcttatg ctaatttgaa gcactagcat
     4021 tctttgatac aatgataatc atttaaacat cacagttata ctacaaatac tatggttaaa
     4081 ctatgtctag tgtagtgtaa cactatggtt gccattgtta tctttaagtt taagtattat
     4141 ctgtggtaaa gccatggttg gtttttataa gaggtaataa aattaacatt gtacaattaa
     4201 atgactataa attaatgttt ttttttattt tttttacaca gctaccttca aaaagggatc
     4261 cactcccttg gcgggccgga tggcggaccc cggcccagat cccgaatctg agtcggaatc
     4321 ggtgttcccg cgggaggtcg ggctctttgc agactcttac tcggagaaga gccagttctg
     4381 tttctgtggg catgtgctga ccatcacgca gaactttggg tcccgcctcg gggtggcggc
     4441 gcgcgtgtgg gacgcggccc tgagcctgtg caattatttc gagagtcaaa atgtggattt
     4501 ccgaggcaag aaggtgatcg aactgggtgc ggggacaggc atcgtgggga tcttggcagc
     4561 gctgcagggg ggggatgtta ccatcactga cctgcccctg gccctagaac agatccaggg
     4621 caacgtccag gccaatgtgc cagctggagg ccaggcccag gtgcgtgcct tgtcctgggg
     4681 gattgaccat catgtcttcc ctgcaaacta tgacctggtg ctgggggctg atatcgtgta
     4741 cctggaaccc accttccctc tgctgctggg gaccctccaa cacctgtgca ggccccatgg
     4801 caccatctat ctggcctcca agatgagaaa ggagcatggg acagagagct tctttcagca
     4861 cctcctgccc cagcatttcc aactggagct ggctcagcgg gatgaggatg aaaatgtcaa
     4921 catctatagg gccaggcaca gggaaccaag acctgcttga catcaccctt gctatcgatg
     4981 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     5041 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     5101 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     5161 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     5221 atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag
     5281 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5341 actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga
     5401 ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     5461 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     5521 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     5581 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     5641 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     5701 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     5761 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     5821 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     5881 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     5941 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     6001 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     6061 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     6121 ttcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac
     6181 tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt
     6241 ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc
     6301 tgataactgc acaagtgctc agattccagc agggtggaaa tgagataaag tgtgcagatg
     6361 gggaggggga cgtgaatgag agatttgagg gatgaaaagg atggatgaac gcattgaaaa
     6421 tagccccttt cacaaaataa taccagtaaa ttgtcatata attaatgacc agacctttac
     6481 ctgtaaatga tgtgatgttc atatacagaa gaatgccaac aatttacaga tgattttaca
     6541 aattcttaat tttccttttc taaatggatt tgttcacaca ttatctcttc aaagcaattg
     6601 aattttctgg aaaagattgg ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac
     6661 atggaatgaa gcaaacagca gcctaagaca ggatggcagg aaaaatgtca catgtttaaa
     6721 ataagagtga aaccaacgac ctggacttat agagactgtg tgtccttagg caaagctctt
     6781 gtacaaacag tgcttgtgac tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg
     6841 cttggatgta tcataaaaaa gaatcggttc tctgtatttg acattttctg taatttatct
     6901 aaagttacaa ctggctatgc ctgattttta tcactattga atcaatcatt ttaaagaatg
     6961 ttaagtctgc tgatttggag agctcattta catttatttt gaatgtctct tatttaacac
     7021 aactgattta agctttagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct
     7081 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
     7141 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
     7201 agatgtgaca tgattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     7261 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
     7321 tccactagtt ctagagcggc cattaccctg ttatccctag gccgccaccg cggtggagct
     7381 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     7441 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7501 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7561 tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     7621 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     7681 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     7741 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     7801 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     7861 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     7921 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     7981 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     8041 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8101 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8161 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8221 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8281 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8341 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8401 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8461 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8521 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8581 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8641 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8701 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8761 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8821 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8881 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8941 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9001 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9061 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9181 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9241 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9301 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9361 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9421 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9481 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9541 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9601 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9661 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9721 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9781 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9841 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9901 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9961 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10021 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    10081 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10141 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    10201 cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaattacc
    10261 ctgttatccc tatcgagcaa aatcaatcct aatgtaaaat agtatacgtt catcattata
    10321 atgcaaacac tgtagaactg ttgtgttgtg ccttgcattt cattctcagt ttcttatcgt
    10381 ctgtctcttt ttacgacaga gctagaaaaa actagccttc atttgccatc gccgtatgca
    10441 aatccactcc agaactcaac aggtttgttc tgcggcgggt gtaagtgtga acaccggcat
    10501 gtaagtgcgc ttgagcagga gaataaagcg ctcagagaca ccggaggaat cctttatttt
    10561 gcgttgcgcc tcaggggatt cgctgagcct tctcttcatg gccctgctct tcagacacag
    10621 gtgctccgct gtttgtgttt ttcactgttc ttcaggcact cgtggttacg ggtttttgtt
    10681 ttgcactctt gcacacacat ctctcggcct ccaaaacaaa cacacaactg cacgagggct
    10741 cgaagccccc accgaaaaac aaaaacatta tgctaatgag gaaggcacag ctgttccctt
    10801 cttcactctc tctctcgttc tttcaacaac agtcgcctaa tttctctcgt tcctttaaag
    10861 agccgccaag cagaaacagt taatgcaaat ataagcctgc gggaaataag gagaattttt
    10921 acaaaatccg agcttcccct cctgccaaaa tgtgatgtgt gactcacgca ttcgatattt
    10981 ggtgtaatga gagtgaatca ttctgctcca caccgaaccc gatgccacag accactcaga
    11041 tgcacaccga ggagactatt ttaacagcac attacatcca gactgtcaca gatgcattac
    11101 caagagtgtg atttcagtgt cgaatgcagg tcttaaagga aaatctgttg cgattgcatg
    11161 tgcgctaact agacacgacc aacgaatggg aacaatctgt gaaatattat acaatcacag
    11221 gcaattaaaa gatgtttaaa gttcaattta gttttaatta atatgttata taaaactaat
    11281 ctgttttaaa tgtatattta tgattttagc tttagctagc ctcttgacca gcagctgaag
    11341 tgtccggcaa gtacctaaaa gggagaccac atgggaaagc taggttgctc ccggaagagg
    11401 tgttagtaaa ccagcaggtc tacctgtggt ctgtgtgggt cctaatgccc cagtataaac
    11461 cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcta
    11521 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtca
//
