LOCUS       sequence-44846-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-HA-Kdrl-3p-FokI-KK
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..35
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             36..125
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 actgcaccat ggctccaaag aagaagcgta aggtataccc atacgatgtt cctgactatg
       61 cgggctatcc ctatgacgtc ccggactatg caggatcgta tccatatgac gttccagatt
      121 acgctgctca tggtacccgc ccatatgctt gccctgtcga gtcctgtgac cgccgcttca
      181 gccgtagcga cgcgctcacc cgccatatcc ggattcacac aggccagaag cccttccagt
      241 gtcgcatctg catgaggaac ttcagctgtg cgcatcacct cacccgccac atccgcaccc
      301 acacaggcga gaagcctttt gcatgcgaca tctgcggcag gaagttcgcg cagttgtcta
      361 acctcacccg ccataccaaa atccatacag gtggatccca actagtcaaa agtgaactgg
      421 aggagaagaa atctgaactt cgtcataaat tgaaatatgt gcctcatgaa tatattgaat
      481 taattgaaat tgccagaaat tccactcagg atagaattct tgaaatgaag gtaatggaat
      541 tttttatgaa agtttatgga tatagaggta aacatttggg tggatcaagg aaaccggacg
      601 gagcaattta tactgtcgga tctcctattg attacggtgt gatcgtggat actaaagctt
      661 atagcggagg ttataatctg ccaattggcc aagcagatga aatgcaacga tatgtcaaag
      721 aaaatcaaac acgaaacaaa catatcaacc ctaatgaatg gtggaaagtc tatccatctt
      781 ctgtaacgga atttaagttt ttatttgtga gtggtcactt taaaggaaac tacaaagctc
//
