LOCUS       sequence-448725-       14904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of AePHOT-Citrine in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Yong LK, Keino I, Kanna Y, Noguchi M, Fujisawa M, Kodama Y
  TITLE     Functional comparison of phototropin from the liverworts Apopellia 
            endiviifolia and Marchantia polymorpha.
  JOURNAL   Photochem Photobiol. 2023 Oct 26. doi: 10.1111/php.13869.
  PUBMED    37882095
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Photochem 
            Photobiol. 2023 Oct 26. doi: 10.1111/php.13869."
COMMENT     Sequence Label: pMpGWB306-AePHOT
FEATURES             Location/Qualifiers
     source          1..14904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(137..153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(137..153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(150..172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(185..191,192..209,210..215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    336..510
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2599..2944)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     gap             3145..3244
                     /estimated_length=100
     misc_feature    3492..3516
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4037..4828
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5074..5662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5563..5582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5816..5833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5848..5988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5914..5933)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6074..6096
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6332..6526
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6592..7665)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8094..8723)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10024..10048
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10236..10258
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10250..10267
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10251..10267
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10782..11127
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    11149..11173
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11380..11391
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(13853..13877)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(13888..13890,13891..13893,13894..14604)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(13933..13954)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(14194..14213)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14541..14562
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      14629..14881
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
       61 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tggttccgga
      121 accaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      241 aactcacatt aggctgaatt aggcgcgcct atttctgaat tcaacacatt gcggacgttt
      301 ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt
      361 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
      421 gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct
      481 gggaactact cacacattat tatggagaaa ctcgagttag tatatagtcc ttccatcacc
      541 ctctgtgata atctccttaa aacttgctcc cccaggaatc ataggcagaa cgtgctcctg
      601 gtggggaact atcacatcca gtaaataagg accgggagaa tctagcatag tttgaattgc
      661 tgctcttagt tcatccttct tcaccacacg cgcatttggc accttgaaac cgtctgcaac
      721 tgtacagaag tcggggtaga tctgctcctc gtgatctgga tcacctaagt aagtatgtgc
      781 tctgttggcc ttgtagaatc tatcctccaa ttgtacgacc attcctagat gttggttgtt
      841 taagatcatg atcttcacgg gtaacttctc cacaaaaatt gtagccaact cctgtacgtt
      901 catcacaaag ctaccatcgc catcgatgtc gatcacagga atatctggtt gagttgcggc
      961 agcccccaaa gcagaaggaa gtccgaatcc catggctcca agacctccag atgtcaacca
     1021 acgccgcggt tcgttgtact tgaaccactg ggctgcccac atttgatgtt ggccaacacc
     1081 agtagatatg atggcatttc caccagtgag ttcgtacagc gtctgtatgg cagattgagg
     1141 agcaatggca tcctcgaata cgggaaactt cattggccat ttctgctttt gttcttctag
     1201 ttctgccacc cagttggaga agtcgaaagt cctcttttga ctcttcaaga gggcattgat
     1261 ttcacttagc gcaggtttca catcagcaca catggacaca tggggctgtt tgttctttcc
     1321 aatttcagca gggtcaatat cgatatgaac gatgctggac ctgctagcaa aagtctccag
     1381 tttaccagtc actcgatcgt cgaaacgcac tccaaatgcc aacaacagat ctgcttggtc
     1441 cacggcgtag ttggcgtaaa cagttccatg cattccaagc attttcaaac ttaatggatc
     1501 agactcggga tacgctccca aacccattaa agtcgatgtg ataggaattc cggtaagctc
     1561 gacaaattcc ctcaactctt cgctggcgtg attgcaacct cctccagaat acactacagg
     1621 cttcttagaa ctagaaatga gtcttataat ctgctcgatt aacgttggtt ccggtggccg
     1681 aggcagtcgt tgtaagtagc ccttcagttg cattggctga gaccaatctg gaatagccat
     1741 ttgctgttgc acgtccttgg gaatatctac caagactggg cccggcctac ctgaagctgc
     1801 caaaaagaaa gcctccctga ttacgcgagg gatgtcatca atgttcataa caaggtaatt
     1861 gtgcttggtg atcgatctcg taacctccac aattggagtt tcttgaaaag catcggtacc
     1921 aatcatccta ctggatactt gacctgtaat ggccactagt ggcacactgt ccaagagggc
     1981 atcagcgagc ccggtaacaa gattggtcgc tccaggaccg gatgtggcta tacaaacccc
     2041 tactcgtcca gacgatttag cataaccttc agcagcgaaa atctccccct gctcgtggcg
     2101 acagagaatg ttgcggatgc tgctggaacg tgtaagagcc tggtgaatct ccatagacgc
     2161 tccgccggga taagcgaaca ccgttctaat cccttctcgc tcaagagcct ccaccaaaat
     2221 gtcggctcct ttgcggggct cgtcatcctt gtagcgagac tgaaagtcgg acggggttgg
     2281 tgcaacgagt gacttcccgc tgctaagccg tggtccactc acatcgactg cagactggat
     2341 cttctcgcta acagcttggg ctttcacttc cagtgaacgc tcagtcttcc gagaggtgtt
     2401 gtatgcatgt cccctcagag cggctcccga gaagaaacca ctcctcacct ttagctcttt
     2461 agctcccttg caacaagacc ttgccgatgg ctgcaccttc tccaggctcg ctatgctcaa
     2521 aggcacagct gcgcatccac taattgacgc catgctcgag agagatagat ttgtagagag
     2581 agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     2641 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     2701 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     2761 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     2821 atcgcaatta tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     2881 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     2941 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     3001 gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     3061 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     3121 catcttgaac gatagccttt ccttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     3301 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     3361 tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gaattcagaa ataaattcag
     3421 cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     3481 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     3541 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     3601 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     3661 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     3721 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     3781 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     3841 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     3901 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     3961 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     4021 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     4081 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     4141 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     4201 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     4261 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     4321 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     4381 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     4441 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     4501 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     4561 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     4621 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     4681 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     4741 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     4801 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     4861 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     4921 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     4981 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5041 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5101 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5161 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5221 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5281 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5641 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5701 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5761 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5821 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     5881 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5941 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     6001 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6061 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6121 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     6181 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     6241 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     6301 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     6361 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     6421 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     6481 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     6541 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     6601 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     6661 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     6721 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     6781 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     6841 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     6901 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     6961 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     7021 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     7081 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     7141 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     7201 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     7261 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     7321 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     7381 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     7441 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     7501 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     7561 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     7621 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     7681 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     7741 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     7801 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     7861 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     7921 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     7981 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     8041 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     8101 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     8161 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     8221 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     8281 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     8341 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     8401 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     8461 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     8521 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     8581 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
     8641 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
     8701 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
     8761 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
     8821 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
     8881 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
     8941 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
     9001 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
     9061 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
     9121 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
     9181 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
     9241 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
     9301 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
     9361 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
     9421 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
     9481 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
     9541 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
     9601 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
     9661 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
     9721 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
     9781 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
     9841 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
     9901 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
     9961 ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct
    10021 taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac
    10081 gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg
    10141 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10201 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10261 ggccagtgcc aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag
    10321 aatgctaacc cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac
    10381 ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa
    10441 agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt
    10501 actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt
    10561 ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata
    10621 gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga
    10681 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc
    10741 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
    10801 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
    10861 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
    10921 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
    10981 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
    11041 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
    11101 ggaagttcat ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac
    11161 aaaaaagcag gcttcatgat gccgtccgcg gagccatccg ttcctccgaa gcctttcaac
    11221 gctcgtaagc cagctgatac ttcatccaaa gtagatgcgt ctcgaactgc tcgcggctct
    11281 ctcgaggttt tcggcggtgc cccctcactt ctgtacacca aacaatcccc agatgttctt
    11341 gttccagcag gatctcggca agggggcttc acggatgaaa tagaaggacg atcaggaaat
    11401 ttggctcaaa ctctcctgaa ggagagggag aagccagcgc ttcccactga gcggcctggg
    11461 agaatagagc cagggaaggc gcgggatcaa gtcgacaagt ggttgtctac tgtggatgaa
    11521 agagtgccac ctccgaccaa gggttttatc agggaaccca gtaatgattt ccttctaaat
    11581 tacagaaaga atggaacccc tttctggaat ctccttacaa tagcacccat taaagacgac
    11641 aatgggaaga ttctgaaata cattggaatg caggtggaag ttagcaagta tactgaagga
    11701 aatagggcaa acgatgtccg acccaatgga atgccagcat ccctgataaa atatgatgct
    11761 cggcagcaag acagggcggc aagttctgtt caagaactgg tcggtgctct caaacatcca
    11821 aatggtaatg gacaccaagc atcacctcca cgaaaaagca ctggtggcgg tgctccacca
    11881 ctgttttcat ttccaccctt gaaagatgag ctacagctga agttaccttc ccgctattca
    11941 actcaatctt ctgcaatggg gaagattcct gaggactata atcagccgaa atttagatca
    12001 aaaagtgaat cagtgaacag aactccagat gaagcagacc cacgttcagt ggggggaaat
    12061 gctgggaaga gccaaagcac tcgtcggcct tccggactga tgcagttgat taagaaacca
    12121 gcaaagcctg caccacctcc accagaagta gatgatcttg atctagatga ttctgtaccg
    12181 aatctggagg acagagaaag tttggataat tcagagcggg ctacggagat tcgtcgaggc
    12241 atggacttag caactactct agagcgaatt gagaagaatt ttgttattac agatccacgt
    12301 ctgcctgaca atcccattat ctttgcatca gatcattttc tagagttgac ggagtacacg
    12361 cgtgaggaaa tcattggacg gaattgtagg tttctgcaag gtcctgacac agatatgggt
    12421 gtggttcgga agattagtga tgcaattaga tctcaacaaa acatcacggt gcaactttta
    12481 aattacacca aaacagggaa gccattctgg aacctttttc atcttgaggc tatgaaggac
    12541 aacaagggag agttgcagta cttcatcgga gttcaacttg atggcagtga gcacattgaa
    12601 ccaatcagac gacgaatgtc agagagaaca gaagaagaag ggaaaaaaat tgtgcaatct
    12661 acggcaaaaa atgttgatgg agctttgaga gagctcccag atgcaaacac gagtctggag
    12721 gacttgtggg caaaccattc acgtcttgtt cttcccaagc ctcacaaatg gaacagcgct
    12781 acatggcatg ccgtacgcaa ggttctgagt actggcgaga aactgggact caatcacttt
    12841 cgaccgatta aacctcttgg ttgtggggat acaggaagtg ttcatttggt cgagctaaga
    12901 aacacgtctg agttttacgc catgaaggcg atggagaagg ctgtgatggt taaccgtaat
    12961 aaggttcatc gtgctcgtgc agaaagagag attcttgaga aaatggatca ccctttcttg
    13021 cccactcttt atggttcttt ccagacacga acacatgtgt gtcttatcac agacttttgc
    13081 cctggtggtg agctttttct cttgttggaa agacaacctc agaagagatt tcaagaggac
    13141 tcggcaaggt tttatgcagc agaaattata ttggctcttg agtatctcca ctgtgtaggg
    13201 gtagtgtaca gggacttgaa acctgagaac gtcttaatac agcgagatgg acatgtacag
    13261 ctcaccgact ttgatctatc ctttttgact gcctcacgac cccagcttta taaaccagct
    13321 gtgccttctg gacgacgtaa gagaagcgta aacaaggaat cattacgccc gatccttctg
    13381 acagaacccc ctgcacggtc aaattcattt gtcgggactg aggaatacat tgctcctgaa
    13441 attattcttg gactcgggca caacagctct gtggactggt gggccctagg gattctcttg
    13501 tatgagatgt tatttggacg aacaccattc cgaggccgta acaggcaaag aacattcgcc
    13561 aatgtgttgc ataaagatct tggttttcca tcatcaatac ctgtgagcat tggtgcaaag
    13621 caagtaatac gtggtctctt gcatagagat cccaagaagc gattgggttc agataaggga
    13681 gccaatgatt tgaagtctca cccctttttc agtgacatca attggcctct tattcgttgc
    13741 atgattcctc caaagctaga aactccagta ttccatattg ggaaggaacc agagactaaa
    13801 gatttggact gggatgatgg ggatccaact gcagcctcct ttgatggtgc tgacccagct
    13861 ttcttgtaca aagtggttga taacagcatg gtgagcaagg gcgaggagct gttcaccggg
    13921 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
    13981 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
    14041 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc
    14101 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
    14161 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
    14221 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
    14281 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
    14341 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
    14401 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
    14461 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
    14521 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
    14581 ctcggcatgg acgagctgta caagtgagct tagagctcga atttccccga tcgttcaaac
    14641 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
    14701 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
    14761 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
    14821 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
    14881 cgggaattgg ttccggaacc aatt
//
