LOCUS       sequence-448768-       12547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 
            splicing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Graceffo E, Pedersen E, Rosario M, Krude H, Schuelke M
  TITLE     Bichromatic Splicing Detector Allows Quantification of THRA1 and 
            THRA2 Splicing Isoforms in Single Cells by Fluorescent Live-Cell 
            Imaging.
  JOURNAL   Int J Mol Sci. 2024 Dec 17;25(24):13512. doi: 10.3390/ijms252413512.
  PUBMED    39769274
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2024-12-17"; volume: "25(24):13512. doi"; pages: " 
            10.3390/ijms252413512"
COMMENT     Sequence Label: pCMV-THRA-RFP-EGFP
FEATURES             Location/Qualifiers
     source          1..12547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3375..4088
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(4334..4345)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(8012..8014,8015..8728)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8054..8075)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8315..8334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    9335..9456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9372..9391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9426..9445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9463..9918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9600..9621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9812..9831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9945..10049
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10051..10408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10051..10242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10074..10094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10259..10394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10443..11237
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10443..11237
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10497..10516)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11107..11126
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11425..11444)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11469..11516
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11845..12433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12334..12353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgccac catgggtccc gatgacattg gccagtcacc cattgtctcc
      661 atgccggacg gagacaaggt ggacctggaa gccttcagcg agtttaccaa gatcatcacc
      721 ccggccatca cccgtgtggt ggactttgcc aaaaaactgc ccatgttctc cgaggtgagt
      781 ggcagaagtg gggagagatg tgatgctgtg ctggagggaa acctgctccc ccggtcaccc
      841 agtacaggct catctgaggt tctctggaca aggagcaatt cctgtaggtc aacatacaca
      901 gataacatac acagacacac acacacacat gcatacacat acacccagca cacaggcaca
      961 catgcagaca catacacgga cacacacaca cacctagcat acagatacgt acacagacac
     1021 acatacacac acacatacac ccagcacaca gacacacaca cacctagtac acaaacacac
     1081 acacagatac atagaaacac acacagacat atacacccag cacacagaca cacacacaca
     1141 cacagacaca cacccagcac acaggcacac acacacacac acctagcaca cagccacaca
     1201 gatacataga cacacacaca cagatacaga cacacacaca cacacagaca tatacaccta
     1261 gcacacagac acacagatac atagacacac acctagcaca cagacacaca cacacagaca
     1321 cacccagcac acaggcacac acacacacac acacctagca gacacacaca tagatacata
     1381 cagacacaca cacaaacaca caggcacaca cacacagcat acagacacac gccaaggtct
     1441 ggctgctcta agaaatcagc cggctccaag tgctcccctc cacagcccct catccttcaa
     1501 gtctatgcgg ccacccaact ccaggcagcc tgaaagttct cattttccag agcactagtg
     1561 ctggagacag gctgccctac tagccatgtg acctggggta agttacctcc atcccctgtg
     1621 cctcagtgtt cccatctgga gtgggctggg gataatgatg ggtgctaact cacaggcata
     1681 attttttttt cttttttgag acagattttc gttctgtcac ccaggctgga gtgcagtgac
     1741 gcgatctggg ctcactgcaa cctccgcctc ctgagttcaa gcaattctcc tgccttagcc
     1801 tcctgagtag ctgggactac aggcatgtgc caccacgccc aactaatttt gtatttttag
     1861 tagagatggg tttcaccatg tatgttggcc aggctggtct cgaactcctg acctcaagtg
     1921 atccacccgc cttggcctcc catagtgctg gcattatagt cgtgagccac tgcgccgggc
     1981 ctcacaggca taactgtgag gcttaaatgg aatagggcat gcacatggcc caatacaagg
     2041 tcagccgttc agagtccatg ggggccttgc gggcctcacg gctcccgtag gacactctag
     2101 gggagactca aggacgcggg gaggggtatg ctgagtgctc ctgtggccct gccgctccac
     2161 agctgccttg cgaagaccag atcatcctcc tgaaggggtg ctgcatggag atcatgtccc
     2221 tgcgggcggc tgtccgctac gaccctgaga gcgacaccct gacgctgagt ggggagatgg
     2281 ctgtcaagcg ggagcagctc aagaatggcg gcctgggcgt agtctccgac gccatctttg
     2341 aactgggcaa gtcactctct gcctttaacc tggatgacac ggaagtggct ctgctgcagg
     2401 ctgtgctgct aatgtcaaca ggtacctgct gattagtcgg gagggctcag aagcttccag
     2461 gggtcccaga gattggctgg accctgggcc tgttgctcaa ctccctcctg aatcttcttc
     2521 tgggctacct ctctgtcacc tgggccatcc cctatcccct gttccttgct ctgtcacaca
     2581 cttctcactc gttccccagg cccatccctt gcctccccat ccctgctctg tgccttttcc
     2641 tgtctttgtc tccttccttc tcgctgccct gtccctctgt cactcacatt cccctttgtt
     2701 ccccactccc cactttgttc tcagccacct tccatcccct catccctcct ctgtctccca
     2761 gtcccatctc ttacccctca gtcccctccc taaccccatc tgcaccccgc ctctctgacc
     2821 accaacctca gtcctcccca ctccaaggct cctctctctc ccccagcccc cggcctgtcc
     2881 ctctgtcctc cttgcattgc cccctccccc cagtaccccc ctgcccctct ccacttccca
     2941 gctgcctccc tctccctgtg cttctcctgt ccacgtctct cagggggagc ttctcccctc
     3001 ccctccccca gcctctctgc ctctatctcc cctctagtcc tttcttccca cgtccccaca
     3061 cctcaccctc cccatctcca ggccttcggc cagcccctcg cccctcacgc ccctcttccc
     3121 tcacagaccg ctcgggcctg ctgtgtgtgg acaagatcga gaagagtcag gaggcgtacc
     3181 tgctggcgtt cgagcactac gtcaaccacc gcaaacacaa cattccgcac ttctggccca
     3241 agctgctgat gaaggtgact gacctccgca tgatcggggc ctgccacgcc agccgcttcc
     3301 tccacatgaa agtcgagtgc cccaccgaac tcttcccccc actcttcctc gaggtctttg
     3361 aggatcagga agtcatggtg tctaagggcg aagagctgat taaggagaac atgcacatga
     3421 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
     3481 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
     3541 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc
     3601 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
     3661 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     3721 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
     3781 tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg
     3841 gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct
     3901 gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
     3961 tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
     4021 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
     4081 ttaattgaag cctcaggcgg ccagagggtg tgcggagctg gtggggagga gcctggagag
     4141 aaggggcaga gctgggggct gagggagacc cccccacacc ccttctctcc ttcctctcgt
     4201 ccttggatag attcagctcc cacacacaca cccgcactgc ccaggtccct cctcagacct
     4261 ccagccctgg gacagggcaa acaactgaac ttgctatgga aaggacagtg tgggaggctg
     4321 ggggagctgt gtcctgcagt tcccaggacc ccatcctctc agaaggtagg ggaagggcgg
     4381 gaggattgag aagggacaag ccaccttgac cgtaggggaa ggaggaatgt gggctggggg
     4441 aagatgccct caactcaccc cctacacaca catgagagag agcccccacc cagttccttg
     4501 gcctaggtct cccctccagg ctgagggcct ctctacttcc ccagatgcct gggtgcaaag
     4561 aacggcttgg cttggctcct cctctggagg ttaaaattta tagtcattct aactgcactt
     4621 tggaaaccaa gcaaggggag aagacaaatg aagaaaaact agacagagag aaaaatacaa
     4681 aaaagagaga gcgagcgata gagagagatg atattaagtt attaactgag gctgaccaga
     4741 ggggaggacc ccccctttac caccccatgc actttgcgag ctgccccttc ttcccccaca
     4801 tcagagagaa atgcccccac accagagccc caagggtagg gctgccgatc agagctgtga
     4861 gttaacacaa cagggtcctg gccacccccc ttgccatacc ccgccctctg tgcccctttg
     4921 gcacccccca accctagtat gtcctctgct ttctgccact cgtgcccgct gagttccatc
     4981 tacctctcat gctggatgcc agctggcccc tcaccagcct gccctgctgc ccacacagct
     5041 cccctttaag tgcccagccc taggggcctc tccccatcca cctcctcctt ccacaccttg
     5101 gcacccaccc aggaaaaaac tgtggctcca actcctaccc tccttatatc ctgcagtatt
     5161 agctagaaac cggatgctgc tgcattggtt gggtcggggg taggagggct gtgcgtgagc
     5221 gtctcccaga accctccacc cctttagcac tctgtttccc tgtttcccat ctgtcttctg
     5281 ggctctctct aacctctgcc ctcctcccca gctcctactc actgcactaa ctctgggcgg
     5341 gcaggtacca gaatgggggt gtttagataa gtgacactta gttggggccc caggggaggg
     5401 gtggtcggtg ctcctttttc tttaaagctc ttttggccag ctgcccctct gccccgggac
     5461 cccttcccct cttcttttct ctaattcagg gactttggct tgagcccctc tcgccctcct
     5521 ggtgagggtg ctctgttggt ctgcacttgg gtgagctgtc ctcctccccc tccctgtggc
     5581 tgccgcccac tcctcagggg agagagggag agaggaggtg gtcctcccat gggagcagga
     5641 ggtggggtgg gacagagcag accagagggt gctgggctca gggctgcatg tcggcaggga
     5701 tggatgggtg gacacagatg cccccggaag ccatggggat tggggcaggg ggtgggggga
     5761 ggggtgccag ggcttcctgc gctttgcact attggggcaa aaatgtctta agcagtgggg
     5821 aacctgccac cccaccctga ccctcagcct gccacagccc cctacacaca cacacacaca
     5881 cacacacaca cacacacaca cacggacatg cacacacgga catgggaagg caatgctatg
     5941 ctgcccgtca gggcactgtc cttccccaat cgttcaggtg ttccaacagg ggtggagggc
     6001 ctggaggagc acccgctgtc acctgtgcat gtgcctgtcc caccctgctg gggcctcggg
     6061 ctgcccgcgc tgtctttgtc tctatcctct ctctctctcc tggagtactg aatcctgtat
     6121 tactccagtc ccatgggtag gccctcttgt accttcctac cacgcctggg ggcccagtgg
     6181 aattcctgac ctgtctgctg tcttccccat ccctccatcc ccaccccaac ccctccatcc
     6241 ccattctcag ccatggacac ggcagagaaa aggccttgaa gagccttagc ctcttatagg
     6301 cacttgggac tcccccaccc tccccaatca tatgagctgt gatcccatcc tccccggacc
     6361 cctcccttcc ccctcggtga tgtggggtgt atgtgtgcgt tcctctgcgt gaatgtgtga
     6421 gtgagtacac atggtagggg aggagccagg gcgactcagg agtgccacgc acctgctcta
     6481 gagcggcagc tgtcccagtc cctgctgtgg aagtggggga cagggccctc tcctgggggc
     6541 cattggtgct aatgaggggg atggccttga tgtgggtgct cagcatgcct cccccagtat
     6601 tggcccgggg cactagggca ggcagggtgg agcagcaggt gcagtatcgg tgggaggacg
     6661 ggttgctggg aataggacga gggggccagg ccaggacaag gggttgccag agccatgacc
     6721 actaccccgc ccccaccacc cgcctctcca tctcagtatt gcccctccca tcactcataa
     6781 cacacatacc tcatccagcc tcctccctgc tcagacttgg ggagggtggg ggaggagccc
     6841 ctaccccttc tcctggtggc gggtctgcag ggctgggaga gggcagggcg ttgtgagaga
     6901 gagaccgtcc ataaggagga cagtaactcc tgccctggga actcctgggc gggggggagg
     6961 gggacactgc ccagaggcgc tactgaatgt atgagaagct ggtgctgggc tggggggagg
     7021 ggggttgtgg ccagaaacag ggaaggaggt aggtctcttc cccagggagg ggtgggaccg
     7081 gcaggggtga cttgaggggc tcctactcct gccccacgtt gacaatcagt atgtctgtta
     7141 tgtgcgattt ttcaccccgt tgtgttttgg gtcaggattt taaagaaaga tatttttatg
     7201 gtaattgttg ctcgtctatt ttactatata tttatgtaat aaatatatga tgaaaataac
     7261 ccccttgggc acccccctta gacttgtgtg ctgtttccct atatcctccc atctgctggc
     7321 agagtaccca ccccacaaac tgaccagatg gagaggtggc cccccccagc cttggcagta
     7381 tttccacccc accccccaaa cgagcacaca ccacagaagc cagctcagct gtgaactatt
     7441 ggatttgaga caggaacaga acaaatcaga gggccagggg agggttgtgg gggagacaga
     7501 gtggtttaaa taggggagga ggggaagttc ggtgatgggg gagggaggca ggtatttaca
     7561 agaaggctca gggggccaga ggctcatctt ggaatatttt ataacaatat aaataagatt
     7621 ctggtttgct tttccttttc gtctcgtaaa ggagagagaa gtgcagagtt cgattctgta
     7681 caagggggca gcggcagaag gccggccggg cgggtcactg ggcgtccacc cggaaggaca
     7741 gcagcttctc ggaatgcatg ttgttcaggg tccgcaggtc cggcagcttg agcagcagct
     7801 tggtgaagcg ggaagtctcc aagggccggt tcttcagcac cagagcccga agagcccgca
     7861 gcagcgtctc ctggagctgc tccaccgaag cggaattctc catgcccgag cggtctgtgg
     7921 ggaagacgac agcagtgagg cggactcccc gagctcctct gaggaggaac cggaggtctg
     7981 cgaggacctg gcaggcaatg cagcctctcc cgtgagcaag ggcgaggagc tgttcaccgg
     8041 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     8101 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     8161 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     8221 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     8281 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     8341 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     8401 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     8461 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     8521 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     8581 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     8641 ccccaacgag aagcgcgatc acatggtcct gaaggagttc gtgaccgccg ccgggatcac
     8701 tctcggcatg gacgagctgt acaagtaaag ccccccagaa ggccgatggg gaaggagaag
     8761 gagtgccata ccttctccca ggcctctgcc ccaagagcag gaggtgcctg aaagctggga
     8821 gcgtgggctc agcagggctg gtcacctccc atcccgtaag accaccttcc cttcctcagc
     8881 aggccaaaca tggccagact cccttgcttt ttgctgtgta gttccctctg cctgggatgc
     8941 ccttccccct ttctctgcct ggcaacatct tacttgtcct ttgaggcccc aactcaagtg
     9001 tcacctcctt ccccagctcc cccaggcaga aatagttgtc tgtgcttcct tggttcatgc
     9061 ttctactgtg acacttatct cactgtttta taattagtcg ggcatgagtc tgtttcccaa
     9121 gctagactgt gtctgaatca tgtctgtatc cccagtgccc ggtgcagggc ctggcataga
     9181 gtaggtactc cataaaaggt gtgttgaatt gaagcggccg cgactctaga tcataatcag
     9241 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     9301 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     9361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9421 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     9481 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     9541 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     9601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     9661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     9721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     9781 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     9841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     9901 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    10021 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
    10081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    10201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    10261 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10381 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
    10441 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
    10501 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
    10561 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
    10621 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
    10681 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
    10741 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
    10801 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
    10861 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
    10921 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
    10981 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
    11041 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
    11101 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
    11161 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    11221 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
    11281 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    11341 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    11401 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
    11461 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
    11521 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    11581 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    11641 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
    11701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    11761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    11821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    11881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    11941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    12001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    12061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    12121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    12181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    12241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    12301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    12361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    12421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    12481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    12541 catgcat
//
