LOCUS       sequence-449143-       11070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing ACC-1 in RIM neuron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Kumar S, Sharma AK, Leifer AM
  TITLE     An inhibitory acetylcholine receptor gates context dependent 
            mechanosensory processing in C. elegans.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204.
  PUBMED    38585821
REFERENCE   2  (bases 1 to 11070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204."
COMMENT     Sequence Label: 
            pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR
FEATURES             Location/Qualifiers
     source          1..11070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6837..7547
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(8492..8514)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8614..8633
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8711..8729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8797..8901
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8902..8970,8971..9762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9120..9139)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9933..10521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10422..10441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10675..10692
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10809..10830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10845..10850,10851..10868,10869..10875)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10883..10899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10888..10910
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10907..10923
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10907..10923
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcagaagc ttgggagcaa cagttattct caagggagag agtattgcag tggttggttt
       61 tttttttgca aggaaggcaa taaaacttga actttctcta aatttcaggc caaggacttt
      121 gcacttcgtc acgctaaaga gaatcactta aagtacatta atggttatga tgcaattgat
      181 attcttgctg gacaaggaac aattggactt gaaattcttg atcaagttcc agatgttgat
      241 acaattcttg taccagttgg aggtggtgga ttaattgctg gaatcgcaac agctgttaag
      301 acattgaagc cggatgttca tatttatgta agctcatttc tcataattgc aaattttaaa
      361 gtaaccgttc gaactatttc cattttaaaa tgaatatttt gtttcttttt cagggaattg
      421 aatcagaaac ctgcccatcg ttcactgaag catatgaagc tggccatata ataacttccc
      481 aggccaaagc gtcacttgct gatggtctcg ccgttcccac agtcggcgga aattctttgg
      541 agacagcaaa aggcttggtt gataaggttt gatagttgat aactgggtca ttacaactga
      601 taagaaatgt tgcaggttat cacagtgaaa gaagaaagca ttgcgctgag tattttaaga
      661 cttttggaag ttgaaaaggc tgttgttgaa ggaggaggag ctgttggatt agcagctatt
      721 ttggaaggaa aagtaccaga attgaaagga aagaaagttg tttcaattct ttccggtgga
      781 aatattgata caacagtact tggaagatca attgaaagag gattagctgt agatggaaga
      841 cttgttcgtt tagaagttgt cgtatcagat agaccaggag gtattgctga actaacaact
      901 acaattgctc atcttggagc atcaatcaaa gatattttcc acgaaagagc atggatctca
      961 acggatgtat tccacgttaa agttaaggtt gtggcagaaa ctcgtggaaa agagcacgtg
     1021 gaggagattg agactgcatt gaagaaaatt tatgataatg ttatacttca ttgaactctc
     1081 tattttcaaa tatattaaaa taaaacgatt tcactgatga atttatgtat tggagcagca
     1141 tactgttaca gtgggctgaa aataaattat ttttaacttt tttttttgga aaaaaccaac
     1201 ttacatttac actttgctgg cacgaataat tatagggaca ctgactataa gtagttggtg
     1261 tacatagttt cggagcacca ttgacaagta atgtcttccc gttgatacaa attaaagaag
     1321 aagcaaggcg acaacataaa aattgggata cttgatttga cggtgcacat gaatatccaa
     1381 ttggacatga tgtgtcaagt acagctgtgc aagtacgaac tgcgttatcc acttcatttt
     1441 tgtaagaatt ccaaccagtc ggacatgaaa ggttttccgg aatcggggtc gaacctaaaa
     1501 ttctaaatag aaaaatcgct aagttatgtg gatttcagtt gacgagccta tctttaatat
     1561 atttacccgt taaacaacac acatctacga acggatcatc agatggtgca caatcataac
     1621 cggatgggca acttgtgata gcactcgtga tttcacaagt ataagtttga ccagcaatct
     1681 gttgataaac cggtcgccca tcagcgcatc cgatcgttga tccagttgac ccttgtccac
     1741 agcaaacata tgttgctaga agtactgata gctggcacgt gtacccggcc tgaaagttgt
     1801 tttttttttg tttcaatcag aataaaataa gataccgatg agcattgtgt tccgggtgtt
     1861 gtgcacatct cgagagctcc atttgtaata agtgcaattt ggttgttaga gcaaattctc
     1921 tgcgagcttg aggatctgca acatatgaat gcagagttga gagtactttg catacacgat
     1981 gaaccagatg gacacgtgga tacagaagga ctacaggata cctaaacatt atttggtatt
     2041 aagattggga agttttaagt tctacaacca accgttcctc catttgaacc aactgctggt
     2101 gtgaaatcag atgggcaaga tgaagttgat attgaagagg aacaacatag aaaagtggaa
     2161 gcaacattag aagattgtac acattgatat ccagttatac aattagtcgg attattttgt
     2221 gagcattcta catatcctat cgtggatggt tgagaaactg cattatttgg gcatattggt
     2281 gtaacaccag aaggttgaga acaacatatt aaaactcctt gaccatttgc agcagtgaca
     2341 catatcgaac tgaaaacaga gttgattttt agaagttaca actttcaatt taatcaaaat
     2401 ctttatcagc aaaaagttac aaaaaaaaac cgaaaaggtt ggttaaattt ttatattcga
     2461 acgctaatgg gatgatgcaa gtgaatttct cgtcatttct cgacaaatat tttgcaatgc
     2521 aagtgtgcgg agaaatgaca tgaaatgtcg tgaaattagc aatttctccg caatttttcc
     2581 gcatttctcg acaattcccg acatgcgaaa aatactcatt tttcgtcaaa aaccacgtca
     2641 ttggttcacg gctttacctt ctcagacttt cacaatattt tatttttcct attttacact
     2701 actttcaaca aacgcgaagt ctgtgaaggt aaagtcgtga actaatgacg tggttctgac
     2761 gaaaaatgag tttttcgcat gtcgggaatt gtcgagaaac gcggaaaaat tgcggagaaa
     2821 ttgcgaattt cacgacattt catgtcattt ctccgcacac ttgcattgca aaaaatttgt
     2881 cgagaaatga cgagaaattc acttgcatca tcccataaca gtatttagat acaaacaatc
     2941 taaactttat aagaatttta aacaatatca cctaacttcg tcggtcaact tccaagatta
     3001 tggtaactga gaacaaaaca tttgagaaat gtttgaaaag atttactatg atatttgttc
     3061 atttttagaa catttttaat ttaaaaaaat ttagaaatca ccttgctgga caatcagtat
     3121 ttgatccaac agatgtacaa aataaattag ttccaccatt tgaaactaac ggatttgcat
     3181 tattcggaca tgtgaacctt ggttgttgcg cacaaatgaa agagaaatag aaggatagaa
     3241 tgaaaagaat cttgtcgtac atttctgtat gagccgcccg tagtggagca tgtttgggaa
     3301 acagaggagc atagagtgac caggagcaca ctacctgaac gcaaatataa atcgcccgtc
     3361 actcccattt tgtttgcatt gttattatcg gggtatatga ctattatcgt gttaaaacag
     3421 gtgtgtagac attttttcct gaatttttca ttcgtgctct gttgaatgga tttaggacag
     3481 atataattac agattcaaag tatcttatgc atagataagt ggaatggatt tataaaaaac
     3541 actcgcaaat ctataaaaaa cactcgcaac ttcctaactc aggaagaaat atgtatggga
     3601 aacgatgact aacattctcc aatggtagaa caataatttt tcggaaattt caggtaacca
     3661 aagaacatga agtcctggaa gtaaaaaaat aattcgtccc cattcggatt tatacaaaac
     3721 atttcttcaa gttcttcccg ttatctcaat aatattctga atcctattaa gcgtcaagag
     3781 cagctttacg tgttataagt ggtttgatgt caattgcata ctcacccgaa ttcgcaacta
     3841 ccgtaggaag ctctagtaga agtgaagaaa agagcgaaaa cgtcatcgtc tattttgaga
     3901 gatttatttt tcaagtcgtt tagttttctt ttttctccac agaatgttga aattttattc
     3961 aacattgcta tttaattgtt taatttttat gtcgaaaaca attttaatcg gctatatcta
     4021 aaatcgtggc gagaccctct aaaaatttta tttatgttcc tttgttacaa aagtgcggga
     4081 aactttaaac tctgaattat tcgaaacgtc ccacatgttt ttcttctgtg aattttgtgt
     4141 ttgttgaccg atttttgatg caactcttat catttttgat tatatttatc agggaacttg
     4201 gttgtccgtg cactttttcg tctcaaatac aaaatctcac ggcgtgacgg acaatttcgc
     4261 gaagcatttt ggtctcggcc caattttcct tctttctttt ttcttcaaac gcataaccat
     4321 aaaaactcca ttagcccagg gaaaagtatt gcagcataaa ttgtgcaaac tcccagtatt
     4381 ggtcaattaa ctgtgcgagc aacggtgcat ttttcaggac cgcccaagga tccccgggat
     4441 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     4501 gaggaccctt ggagggtacc gagctcagaa aaaatgagtc atccgggttg gattatggtg
     4561 tcgttcctaa ccgaattact atcacaatca tcaaaaggaa tagctcagtc actggataat
     4621 tgtgcaaatg acacagaaat catcaatgca ctactcaaag atagctacaa taaacattat
     4681 atcccttctc atcctacaca tgttcgagtg gatatgtggg ttcaggaagt aacatcagtt
     4741 tcagaattaa cgcaggattt tgaaatagat ttgtatatca acgaattttg ggaagatcct
     4801 gcattagtat acgaagatat gaatccatgt aagcgaaata tatcatttga tgataaggtc
     4861 ttacaacgat tatggttgcc gaacacttgc ttcatcaatt caaaaagtgc agcaattcac
     4921 gaatctccat tcaagaacgt cttcctgatg gttttctcaa atggtactct ctggacaaac
     4981 tatcgaatga aattaacagg accttgtgat atgaaactaa aaagatttcc gttcgataag
     5041 cagaaatgct atctcacttt tgaatctttc aactacaata ctggagaagt gagaatgcag
     5101 tggaatcaac cgtatccagt aattctgctc aaaagaattg aacttcctga tttcaaatta
     5161 gtcaatttta gtgttattgc tgttgaacag atgtacccag ctggttggtg ggatgagctt
     5221 actgtagcgt ttgtattcga acgaagatat ggttggtatg ttcttcaagg atatattcct
     5281 accatggtga ccattgttat ctcttggatt tcattttatt tgggaccaag agcaatccca
     5341 gctaggacta tgcttggtgt caactctctt ctagcaatga cttttcagtt tggaaatatc
     5401 attcgaaatt tgccacgtgt atcttatgta aaagcaattg atgtttggat gttatctgga
     5461 atgcttttta tatttttatc acttcttgaa ttggcagtag ttggatttat gtcaaggaat
     5521 gaaggtctac cgcctaaagt caaaaaacgg aaacgacaag aagatgatga tgaaggattt
     5581 tcatggaaaa gtatgcaaac tagtccgcac ttggaattaa gacagttttg ggtagacaaa
     5641 cgggtgaatt cattacggaa tgacagcgca gtgccgcctg ttgaagatta tgcgccaatg
     5701 gaactcgaac aaccgtatca aaatattaca aaacgaagag aaaaacggaa atggatgagt
     5761 gggttgagga aaaaatggag ggcgatgagg gaattacggc ctgaaacagt agatttttat
     5821 agcgcaatct tcttcccaac agcgtatatg cttttcaaca tttcatattg gagtttctac
     5881 ctgacaagtc tttccgaata ttttgatgaa gatgtgaata ttgatcaacc ttaaggtacc
     5941 gctgtctcat cctactttca cctagttaac tgcttgtctt aaaatctatg cttctcttta
     6001 gtatctaaaa ttttcctaga agcttacaag tatataaatg gtctcttctc aataaaggtt
     6061 gtatatttat tcatcttatt gaatctgcca tttcctcgtt tttgcgagtt tatatacctt
     6121 ccaattttct ttctattgta ttttcaactt ctaattttaa ttcagggaaa ctgcttcaac
     6181 gcatcatgtc tgattccgct gttgttgccg ccgctgtcga gccaaaggtc ccaaaggcta
     6241 aggccgccaa ggccgccaag ccagccgcca aggccgccaa gccaaccaaa gttgccaagg
     6301 ccaaggctcc agtcgctcac ccaccataca tcaacatgat caaggaggcc atcaagcagc
     6361 tcaaggaccg caaaggagct tccaagcagg caatcctcaa gttcatctcc caaaactaca
     6421 agctcggaga caatgtcatc cagatcaatg ctcatctccg tcaggctctc aagcgtggtg
     6481 tcaccagcaa ggcccttgtt caagccgccg gatccggagc caacggacgt ttccgtgtgc
     6541 cagagaaggc cgccgccgcc aagaagccag cagcagccaa gaagccagca gcagccaaga
     6601 aaccagcagc cgccaagaaa gccactggag agaagaaggc aaagaagcca gccgctgcca
     6661 agccaaagaa ggccgccacc ggagataaga aggtcaagaa ggccaagagc ccaaagaagg
     6721 ttgccaagcc agctgccaag aaggtcgcca agtctccagc caagaaggcc gctccaaaga
     6781 agatcgccaa gccagctgcc aagaaggccg ccaagccagc cgccaaggcc gcatgcatgg
     6841 tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca
     6901 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     6961 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     7021 tggctaccag cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg
     7081 acttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     7141 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     7201 acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     7261 tcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg gaaggcagaa
     7321 ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat
     7381 acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca
     7441 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg
     7501 tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaattcc ggactcagat
     7561 ctcgagctca agcttcgaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     7621 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     7681 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     7741 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     7801 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     7861 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     7921 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     7981 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     8041 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     8101 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     8161 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     8221 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     8281 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     8341 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     8401 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     8461 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8521 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8581 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8641 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8701 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     8761 tcttagacgt caggtggcac ttttcgtgga aatgtgcgcg gaacccctat ttgtttattt
     8821 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8881 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8941 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9001 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9061 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9121 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     9181 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9241 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     9301 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9361 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9421 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     9481 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9541 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9601 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9661 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     9721 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     9781 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9841 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9901 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9961 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10021 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10081 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10141 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10201 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10261 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10321 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    10381 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    10441 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10501 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    10561 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    10621 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    10681 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    10741 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    10801 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    10861 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    10921 gaccatgatt acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt
    10981 taaattttca gagcttaaaa atggctgaaa tcactcacaa cgatggatac gctaacaact
    11041 tggaaatgaa ataagcttgc atgcctgcag
//
