LOCUS       sequence-449196-       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing LGC-47 in RIM neuron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Kumar S, Sharma AK, Leifer AM
  TITLE     An inhibitory acetylcholine receptor gates context dependent 
            mechanosensory processing in C. elegans.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204.
  PUBMED    38585821
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204."
COMMENT     Sequence Label: 
            pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6666..7376
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(8321..8343)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8443..8462
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8540..8558)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8626..8730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8731..8799,8800..9591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8949..8968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9762..10350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10251..10270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10504..10521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10638..10659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10674..10679,10680..10697,10698..10704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10712..10728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10717..10739
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10736..10752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10736..10752
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcagaagc ttgggagcaa cagttattct caagggagag agtattgcag tggttggttt
       61 tttttttgca aggaaggcaa taaaacttga actttctcta aatttcaggc caaggacttt
      121 gcacttcgtc acgctaaaga gaatcactta aagtacatta atggttatga tgcaattgat
      181 attcttgctg gacaaggaac aattggactt gaaattcttg atcaagttcc agatgttgat
      241 acaattcttg taccagttgg aggtggtgga ttaattgctg gaatcgcaac agctgttaag
      301 acattgaagc cggatgttca tatttatgta agctcatttc tcataattgc aaattttaaa
      361 gtaaccgttc gaactatttc cattttaaaa tgaatatttt gtttcttttt cagggaattg
      421 aatcagaaac ctgcccatcg ttcactgaag catatgaagc tggccatata ataacttccc
      481 aggccaaagc gtcacttgct gatggtctcg ccgttcccac agtcggcgga aattctttgg
      541 agacagcaaa aggcttggtt gataaggttt gatagttgat aactgggtca ttacaactga
      601 taagaaatgt tgcaggttat cacagtgaaa gaagaaagca ttgcgctgag tattttaaga
      661 cttttggaag ttgaaaaggc tgttgttgaa ggaggaggag ctgttggatt agcagctatt
      721 ttggaaggaa aagtaccaga attgaaagga aagaaagttg tttcaattct ttccggtgga
      781 aatattgata caacagtact tggaagatca attgaaagag gattagctgt agatggaaga
      841 cttgttcgtt tagaagttgt cgtatcagat agaccaggag gtattgctga actaacaact
      901 acaattgctc atcttggagc atcaatcaaa gatattttcc acgaaagagc atggatctca
      961 acggatgtat tccacgttaa agttaaggtt gtggcagaaa ctcgtggaaa agagcacgtg
     1021 gaggagattg agactgcatt gaagaaaatt tatgataatg ttatacttca ttgaactctc
     1081 tattttcaaa tatattaaaa taaaacgatt tcactgatga atttatgtat tggagcagca
     1141 tactgttaca gtgggctgaa aataaattat ttttaacttt tttttttgga aaaaaccaac
     1201 ttacatttac actttgctgg cacgaataat tatagggaca ctgactataa gtagttggtg
     1261 tacatagttt cggagcacca ttgacaagta atgtcttccc gttgatacaa attaaagaag
     1321 aagcaaggcg acaacataaa aattgggata cttgatttga cggtgcacat gaatatccaa
     1381 ttggacatga tgtgtcaagt acagctgtgc aagtacgaac tgcgttatcc acttcatttt
     1441 tgtaagaatt ccaaccagtc ggacatgaaa ggttttccgg aatcggggtc gaacctaaaa
     1501 ttctaaatag aaaaatcgct aagttatgtg gatttcagtt gacgagccta tctttaatat
     1561 atttacccgt taaacaacac acatctacga acggatcatc agatggtgca caatcataac
     1621 cggatgggca acttgtgata gcactcgtga tttcacaagt ataagtttga ccagcaatct
     1681 gttgataaac cggtcgccca tcagcgcatc cgatcgttga tccagttgac ccttgtccac
     1741 agcaaacata tgttgctaga agtactgata gctggcacgt gtacccggcc tgaaagttgt
     1801 tttttttttg tttcaatcag aataaaataa gataccgatg agcattgtgt tccgggtgtt
     1861 gtgcacatct cgagagctcc atttgtaata agtgcaattt ggttgttaga gcaaattctc
     1921 tgcgagcttg aggatctgca acatatgaat gcagagttga gagtactttg catacacgat
     1981 gaaccagatg gacacgtgga tacagaagga ctacaggata cctaaacatt atttggtatt
     2041 aagattggga agttttaagt tctacaacca accgttcctc catttgaacc aactgctggt
     2101 gtgaaatcag atgggcaaga tgaagttgat attgaagagg aacaacatag aaaagtggaa
     2161 gcaacattag aagattgtac acattgatat ccagttatac aattagtcgg attattttgt
     2221 gagcattcta catatcctat cgtggatggt tgagaaactg cattatttgg gcatattggt
     2281 gtaacaccag aaggttgaga acaacatatt aaaactcctt gaccatttgc agcagtgaca
     2341 catatcgaac tgaaaacaga gttgattttt agaagttaca actttcaatt taatcaaaat
     2401 ctttatcagc aaaaagttac aaaaaaaaac cgaaaaggtt ggttaaattt ttatattcga
     2461 acgctaatgg gatgatgcaa gtgaatttct cgtcatttct cgacaaatat tttgcaatgc
     2521 aagtgtgcgg agaaatgaca tgaaatgtcg tgaaattagc aatttctccg caatttttcc
     2581 gcatttctcg acaattcccg acatgcgaaa aatactcatt tttcgtcaaa aaccacgtca
     2641 ttggttcacg gctttacctt ctcagacttt cacaatattt tatttttcct attttacact
     2701 actttcaaca aacgcgaagt ctgtgaaggt aaagtcgtga actaatgacg tggttctgac
     2761 gaaaaatgag tttttcgcat gtcgggaatt gtcgagaaac gcggaaaaat tgcggagaaa
     2821 ttgcgaattt cacgacattt catgtcattt ctccgcacac ttgcattgca aaaaatttgt
     2881 cgagaaatga cgagaaattc acttgcatca tcccataaca gtatttagat acaaacaatc
     2941 taaactttat aagaatttta aacaatatca cctaacttcg tcggtcaact tccaagatta
     3001 tggtaactga gaacaaaaca tttgagaaat gtttgaaaag atttactatg atatttgttc
     3061 atttttagaa catttttaat ttaaaaaaat ttagaaatca ccttgctgga caatcagtat
     3121 ttgatccaac agatgtacaa aataaattag ttccaccatt tgaaactaac ggatttgcat
     3181 tattcggaca tgtgaacctt ggttgttgcg cacaaatgaa agagaaatag aaggatagaa
     3241 tgaaaagaat cttgtcgtac atttctgtat gagccgcccg tagtggagca tgtttgggaa
     3301 acagaggagc atagagtgac caggagcaca ctacctgaac gcaaatataa atcgcccgtc
     3361 actcccattt tgtttgcatt gttattatcg gggtatatga ctattatcgt gttaaaacag
     3421 gtgtgtagac attttttcct gaatttttca ttcgtgctct gttgaatgga tttaggacag
     3481 atataattac agattcaaag tatcttatgc atagataagt ggaatggatt tataaaaaac
     3541 actcgcaaat ctataaaaaa cactcgcaac ttcctaactc aggaagaaat atgtatggga
     3601 aacgatgact aacattctcc aatggtagaa caataatttt tcggaaattt caggtaacca
     3661 aagaacatga agtcctggaa gtaaaaaaat aattcgtccc cattcggatt tatacaaaac
     3721 atttcttcaa gttcttcccg ttatctcaat aatattctga atcctattaa gcgtcaagag
     3781 cagctttacg tgttataagt ggtttgatgt caattgcata ctcacccgaa ttcgcaacta
     3841 ccgtaggaag ctctagtaga agtgaagaaa agagcgaaaa cgtcatcgtc tattttgaga
     3901 gatttatttt tcaagtcgtt tagttttctt ttttctccac agaatgttga aattttattc
     3961 aacattgcta tttaattgtt taatttttat gtcgaaaaca attttaatcg gctatatcta
     4021 aaatcgtggc gagaccctct aaaaatttta tttatgttcc tttgttacaa aagtgcggga
     4081 aactttaaac tctgaattat tcgaaacgtc ccacatgttt ttcttctgtg aattttgtgt
     4141 ttgttgaccg atttttgatg caactcttat catttttgat tatatttatc agggaacttg
     4201 gttgtccgtg cactttttcg tctcaaatac aaaatctcac ggcgtgacgg acaatttcgc
     4261 gaagcatttt ggtctcggcc caattttcct tctttctttt ttcttcaaac gcataaccat
     4321 aaaaactcca ttagcccagg gaaaagtatt gcagcataaa ttgtgcaaac tcccagtatt
     4381 ggtcaattaa ctgtgcgagc aacggtgcat ttttcaggac cgcccaagga tccccgggat
     4441 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     4501 gaggaccctt ggagggtacc gagctcagaa aaaatgctct ccacattgtt tctgttgctc
     4561 tatgccaccc gggaaacggt caatgcaatg actgccgaga tcagcccaac tgtttcttat
     4621 ttattagatc gaggttgtgc aaatgatacg gaaattattg atcatcttct tatcgaccgt
     4681 gctctatact acaataaaca taagctacca tctccacaag tagacgtgag gattgagatg
     4741 tgggtacaag aagtgacatc agtttccgag ctaacgcaag attttgaaat agatttatac
     4801 atgaacgaat attggacaga ccccgggtta gcctacgaca ttctcaatcc ttgtcagggc
     4861 aatctatcat ttgattgggc tgtcatgcag aatatctgga ctccaaacac atgcttcatc
     4921 aattccaaaa aggcacaact tcacagctcg ccatttacta atgttttctt aatggtcttt
     4981 ccaaatggct cagtgtggtc aaattggagg ataaagagta cgggcccttg tgtgatggat
     5041 ctgacaaagt ttccaatgga cagtatcgaa tgtacactga cttttgaatc attcaactac
     5101 aacaaggacg aagttttcat gcgatggggc gatcctccat taacgatctt caaacccatc
     5161 gaactacctg atttcacaat gacaaacttt agtacttcaa ataaatttca gctttatgct
     5221 gccggctact ggagtgaact caatgtgaac tttctcttcc gccgaaggtc tggatggtat
     5281 ctcttgcaag gttacattcc aacctatatg acagtgttca tttcttggat tccattttac
     5341 ttgggcccaa aagcaatacc cgctcggact atgataggtg tcaatgccct actggcgctt
     5401 accttccagt ttggtaatat aatccgaaac ctaccacgag tgtcgtatat caaagccatc
     5461 gacgtctgga tgttgagtgg catatgcttt gtttttgcat ctttgtttga acttgcaacc
     5521 atcggttttt tgatgcgaaa tgaaggcaaa ccggcgacaa aatcaagccg ttcaactaaa
     5581 aattctaatc gttggcaaca accaagtgtt tgccgagaag acactcaaag aaagtcacaa
     5641 aatatcgtat tctgtgagcg attagacaac acgtcaagat taatgtttcc agtagtgttc
     5701 acattctaca acttcatata ttggtttgtt tacatgaaac aagcatggga tcaggcacca
     5761 taaggtaccg ctgtctcatc ctactttcac ctagttaact gcttgtctta aaatctatgc
     5821 ttctctttag tatctaaaat tttcctagaa gcttacaagt atataaatgg tctcttctca
     5881 ataaaggttg tatatttatt catcttattg aatctgccat ttcctcgttt ttgcgagttt
     5941 atataccttc caattttctt tctattgtat tttcaacttc taattttaat tcagggaaac
     6001 tgcttcaacg catcatgtct gattccgctg ttgttgccgc cgctgtcgag ccaaaggtcc
     6061 caaaggctaa ggccgccaag gccgccaagc cagccgccaa ggccgccaag ccaaccaaag
     6121 ttgccaaggc caaggctcca gtcgctcacc caccatacat caacatgatc aaggaggcca
     6181 tcaagcagct caaggaccgc aaaggagctt ccaagcaggc aatcctcaag ttcatctccc
     6241 aaaactacaa gctcggagac aatgtcatcc agatcaatgc tcatctccgt caggctctca
     6301 agcgtggtgt caccagcaag gcccttgttc aagccgccgg atccggagcc aacggacgtt
     6361 tccgtgtgcc agagaaggcc gccgccgcca agaagccagc agcagccaag aagccagcag
     6421 cagccaagaa accagcagcc gccaagaaag ccactggaga gaagaaggca aagaagccag
     6481 ccgctgccaa gccaaagaag gccgccaccg gagataagaa ggtcaagaag gccaagagcc
     6541 caaagaaggt tgccaagcca gctgccaaga aggtcgccaa gtctccagcc aagaaggccg
     6601 ctccaaagaa gatcgccaag ccagctgcca agaaggccgc caagccagcc gccaaggccg
     6661 catgcatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca
     6721 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     6781 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     6841 tcgacatcct ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg
     6901 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     6961 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     7021 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     7081 agaaaacact cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctgg
     7141 aaggcagaac cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca
     7201 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     7261 tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg
     7321 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaattccg
     7381 gactcagatc tcgagctcaa gcttcgaatt ccaactgagc gccggtcgct accattacca
     7441 acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt aaactgagtt
     7501 ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc tctgacttct
     7561 aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat
     7621 ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac
     7681 ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa
     7741 aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac acaatgttct
     7801 gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac
     7861 cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct
     7921 tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta
     7981 tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt
     8041 tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac
     8101 aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg
     8161 gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta
     8221 aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg
     8281 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     8341 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8401 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     8461 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8521 gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8581 ataatggttt cttagacgtc aggtggcact tttcgtggaa atgtgcgcgg aacccctatt
     8641 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8701 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8761 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8821 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8881 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8941 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     9001 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9061 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9121 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9181 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9241 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9301 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9361 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9421 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9481 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9541 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9601 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9661 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9721 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9781 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9841 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9901 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9961 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10021 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10081 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10141 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10201 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10261 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10321 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10381 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10441 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10501 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10561 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    10621 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10681 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10741 aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta ctaactaact
    10801 attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac gatggatacg
    10861 ctaacaactt ggaaatgaaa taagcttgca tgcctgcag
//
