LOCUS       sequence-44924-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-BRAF
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcnat gcttttttat
       61 aatgccaact ttgtacaaaa aagttggcac catggcggcg ctgagcggtg gcggtggtgg
      121 cggcgcggag ccgggccagg ctctgttcaa cggggacatg gagcccgagg ccggcgccgg
      181 cgccggcgcc gcggcctctt cggctgcgga ccctgccatt ccggaggagg tgtggaatat
      241 caaacaaatg attaagttga cacaggaaca tatagaggcc ctattggaca aatttggtgg
      301 ggagcataat ccaccatcaa tatatctgga ggcctatgaa gaatacacca gcaagctaga
      361 tgcactccaa caaagagaac aacagttatt ggaatctctg gggaacggaa ctgatttttc
      421 tgtttctagc tctgcatcaa tggataccgt tacatcttct tcctcttcta gcctttcagt
      481 gctaccttca tctctttcag tttttcaaaa tcccacagat gtggcacgga gcaaccccaa
      541 gtcaccacaa aaacctatcg ttagagtctt cctgcccaac aaacagagga cagtggtacc
      601 tgcaaggtgt ggagttacag tccgagacag tctaaagaaa gcactgatga tgagaggtct
      661 aat
//
