LOCUS       sequence-44925-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-BRAF
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat
       61 aatgccaact ttgtacaaga aagttggcaa gtggacagga aacgcaccat atccccctgc
      121 ctggatgggt gtttttggag aagcacaagc atatagacta aaatcctctg tttggaaacc
      181 agcccgattc aaggagggtt ctgatgcact gcggtgaatt tttggcaatg agcgggccag
      241 cagctcaata gaggcgagaa tttggggaaa gagtggtctc tcatctcttt tctttttgag
      301 gcactctgcc attaatctct tcatggcttt tggacagtta ctccgtacct tactgagatc
      361 tggagacagg tatcctcgtc ccaccataaa aattatctgg tccctgttgt tgatgtttga
      421 ataaggtaac tgtccagtca tcaattcata cagaacaatt ccaaatgcat atacatctga
      481 ctgaaagctg tatggatttt tatcttgcat tctgatgact tctggtgcca tccacaaaat
      541 ggatccagac aactgttcaa actgatggga cccactccat cgagatttca ctgtagctag
      601 accaaaatca cctattttta ctgtgaggtc ttcatgaaga aatatattat tactcttgag
      661 gtctctgtgg
//
