LOCUS       sequence-449278-        8814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing LGC-47 in AVA neuron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Kumar S, Sharma AK, Leifer AM
  TITLE     An inhibitory acetylcholine receptor gates context dependent 
            mechanosensory processing in C. elegans.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204.
  PUBMED    38585821
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204."
COMMENT     Sequence Label: pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR
FEATURES             Location/Qualifiers
     source          1..8814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4494..4522)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5270..5290
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6236..6258)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6358..6377
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6455..6473)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6541..6645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6646..6714,6715..7506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6864..6883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7677..8265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8166..8185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8419..8436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8553..8574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8589..8594,8595..8612,8613..8619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8627..8643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8632..8654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8651..8667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8651..8667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aatgcgaccc atcatattgt tcctattgtc atgtacaagc tgtccctgtt ttgctcgctt
       61 aatccgtcaa ccccttcccc gttctaatta gagatgagtg aattacaata gagttgaaac
      121 ggaaatctga gtgtctgtag ctttccgccg ccgcttttgt tacgagatgg tagacgtccc
      181 tttcttggtg tttattgtct cacaaactgt ataataaagc accgttgtgt aaaaggcatt
      241 tggtatcagg aagataaaat gaataaaatg caaatgcgga atcaaaaatt gccaaataat
      301 tttgaccaat aaaatatcaa aaataataga acagaaataa acaaataaaa ctacggagaa
      361 aatgtgatta ttccattaaa caacgtttta aaccaacttt attcgtcagt ggggaaggtt
      421 ttggacaaat accatagtaa aacttgttcg ttttaattat acataatcaa taattatagg
      481 tgttagaacc aatggtcgaa atttaaaaaa aaatcaacat tttagaaaat ttcggcaatt
      541 taccgaaata ttgactgaca attttacata tataagtgtt ttgaaataga atccccattg
      601 acgatgcgcc accttaaaca gtagaactgt tccaatcaga aactaaataa atacggttat
      661 tatatatata tatatatata tatatatata acttgttttt attatgcatt tctcaaaact
      721 caccaaactc aatctctaat ttacggttgt tgaggaaaat atatttatca catttagttg
      781 agactctaaa agaagtacaa cttttttctt gcaattcctc caccaatcca atttttcaag
      841 agtcaattgc ttggagtcct cgttttctcg taatccgata ttccccctat ttctgtgatt
      901 ttattcagtt tcgtcctctt acaccaaaaa tcttcatgac ttttttggtt ttctgcttaa
      961 tctttttttt gtttttaaag tctaccatcc ggcaattttt tttgcaaatg gttgctcaga
     1021 atttgacctc agattaaaat tttcaggaac ttcctggtcc catttcgtca catttaataa
     1081 caaaccaaat ccttcagctt gagtcgtcca agccaacgcg accctaattc gattccacct
     1141 ccaatttacc tttttgccgt tttcgatcaa attgtggggg ctctttttat ttgaaagtcg
     1201 taaaatggcg cgtccttgtc gaaaattgag atagctaatg gttccaaata aaatcaaatg
     1261 catgaaccta gtaggaaaaa tcgttttttt tttcatcttc caaagaaaaa gttcaagata
     1321 aacccaccac tccagcaatc cacctacgcg tttttcaccg gaaactgtca tttgtttttg
     1381 atgtatgaca gtgccagcac tgaactaaat tcccatttgt tttgttgtgg tgttatctaa
     1441 ccacgtggct ctttctctaa attttgcgta ggctgcaaga tccgcaatcc caccaaaaaa
     1501 tggtttctaa tgtacttcat acatatccca catgactcag tgctccgaaa cgcccagcaa
     1561 cccccgcgtt tgtctttaca aaaatgaggc ggaaatggga aattttcctg ctattgctat
     1621 ggtaaaatac ttaagctgta ttgagatttg ggaaattgaa agataagatg aaaaatccac
     1681 tctgtataat taaaaaggtt tatttttttt gtaatttttg taccgcaaat tttttcatta
     1741 actgattttt ctttattcat tcgatggccc agtttcagat cttcattatc atgtagttta
     1801 ttcattcaaa ctatttgtga catcatgatg gttgtttaaa aataatttcg aatgaagaaa
     1861 gcttaaacct attataattt tcttttctat aaaaaatatt gattttgtaa agcgtttggc
     1921 agtgacagta ccaaaaaatg ttttaaaatg atttaagaaa attttttatt ttatttgtat
     1981 aatacgcgta aaatgagtat actcgtcatt tgtcacgaat cgagttctca gtttgctgtg
     2041 aatatcttgt aatagaaatc gatctcatag tgtcactttt gaggcataac ttttcaggtt
     2101 gttacaagtt cattaacttg tcaagactgg gtcatgtttt aaagtgtaac tgttacccat
     2161 gtaccctaaa ctttgagaca aactcctcaa aacttgtaac cagaatccta tgtagatgac
     2221 tcacaaaacc ctgttttcaa tttgaacgaa tttcgaaact ggccaaaatg ttcccctctt
     2281 gctcacctaa tgcttttacc atttaaaaag gatggtaaca acactctttc gaaaatgtag
     2341 gcggtccatt ttcggtttct cataaccgtc tggctctatc tcctagtgcg cgaggagagg
     2401 gcgggcggag taggaatcgg gggcagcgca ctcatacact ctgcccttct cttttgcaca
     2461 cacagaaaaa gatagataca gaccaacact tattgttgtc cagtaagctt ccagaaaact
     2521 attttcagaa ttacaaacta catttttcta gaacagtcaa ttgatttttt acaagattcg
     2581 agtattctaa cattagtgaa agtatttagc cactgcaatt ttcaactgta ggtttcaact
     2641 tccaaaaaaa attcctagca tttctaattg gatgaaaaaa ggctatctag accctccatt
     2701 atttactcat aatttcatct cgtcctcatt tttaatcccc tcattgatcc catcacaatc
     2761 cgccaaaatg ttttttaaac acttttttct tcaccagccg tatgtggtca actgcctaaa
     2821 aatttcacag ctatttgtca ttccttgcag aagaacttca ttcttctttt tcgaaaggat
     2881 ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
     2941 cattttcagg aggacccttg gagggtaccg agctcagaaa aaatgctctc cacattgttt
     3001 ctgttgctct atgccacccg ggaaacggtc aatgcaatga ctgccgagat cagcccaact
     3061 gtttcttatt tattagatcg aggttgtgca aatgatacgg aaattattga tcatcttctt
     3121 atcgaccgtg ctctatacta caataaacat aagctaccat ctccacaagt agacgtgagg
     3181 attgagatgt gggtacaaga agtgacatca gtttccgagc taacgcaaga ttttgaaata
     3241 gatttataca tgaacgaata ttggacagac cccgggttag cctacgacat tctcaatcct
     3301 tgtcagggca atctatcatt tgattgggct gtcatgcaga atatctggac tccaaacaca
     3361 tgcttcatca attccaaaaa ggcacaactt cacagctcgc catttactaa tgttttctta
     3421 atggtctttc caaatggctc agtgtggtca aattggagga taaagagtac gggcccttgt
     3481 gtgatggatc tgacaaagtt tccaatggac agtatcgaat gtacactgac ttttgaatca
     3541 ttcaactaca acaaggacga agttttcatg cgatggggcg atcctccatt aacgatcttc
     3601 aaacccatcg aactacctga tttcacaatg acaaacttta gtacttcaaa taaatttcag
     3661 ctttatgctg ccggctactg gagtgaactc aatgtgaact ttctcttccg ccgaaggtct
     3721 ggatggtatc tcttgcaagg ttacattcca acctatatga cagtgttcat ttcttggatt
     3781 ccattttact tgggcccaaa agcaataccc gctcggacta tgataggtgt caatgcccta
     3841 ctggcgctta ccttccagtt tggtaatata atccgaaacc taccacgagt gtcgtatatc
     3901 aaagccatcg acgtctggat gttgagtggc atatgctttg tttttgcatc tttgtttgaa
     3961 cttgcaacca tcggtttttt gatgcgaaat gaaggcaaac cggcgacaaa atcaagccgt
     4021 tcaactaaaa attctaatcg ttggcaacaa ccaagtgttt gccgagaaga cactcaaaga
     4081 aagtcacaaa atatcgtatt ctgtgagcga ttagacaaca cgtcaagatt aatgtttcca
     4141 gtagtgttca cattctacaa cttcatatat tggtttgttt acatgaaaca agcatgggat
     4201 caggcaccat aaggtaccgc tgtctcatcc tactttcacc tagttaactg cttgtcttaa
     4261 aatctatgct tctctttagt atctaaaatt ttcctagaag cttacaagta tataaatggt
     4321 ctcttctcaa taaaggttgt atatttattc atcttattga atctgccatt tcctcgtttt
     4381 tgcgagttta tataccttcc aattttcttt ctattgtatt ttcaacttct aattttaatt
     4441 cagggaaact gcttcaacgc atcatgagta aaggagaaga acttttcact ggagttgtcc
     4501 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     4561 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     4621 tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     4681 ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg agatacccag
     4741 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     4801 gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta
     4861 ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa ggtgataccc
     4921 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     4981 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
     5041 atggaatcaa agttgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     5101 ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     5161 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     5221 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     5281 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taggcgccgg
     5341 tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta
     5401 agtttaaact gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc
     5461 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     5521 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     5581 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     5641 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     5701 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     5761 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     5821 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     5881 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     5941 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     6001 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     6061 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     6121 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta
     6181 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     6241 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     6301 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     6361 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6421 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     6481 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6661 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6721 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6781 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6841 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6901 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6961 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7021 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7081 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7141 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7201 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7261 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7321 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7381 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7441 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7501 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7561 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7801 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8341 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8401 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8461 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8581 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8641 caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga
     8701 tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc
     8761 acaacgatgg atacgctaac aacttggaaa tgaaataagc ttgcatgcct gcag
//
