LOCUS       sequence-449297-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the A71 3C protease (isoform B) used in 
            crystallisation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Enterovirus A71 (EV-A71) isolate TW-2553-1998 ...
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggttcga gtcatcacca ccaccaccac atggcgtcca tgtccgatag cgaagttaac
       61 caggaagcca aacctgaggt gaaacctgag gtcaagcctg agactcatat aaacttgaaa
      121 gtttccgacg gcagttccga aatcttcttc aagattaaga agactacacc gttacggcgg
      181 ttgatggaag cattcgccaa gcgacaaggc aaagagatgg actctcttcg ttttctctac
      241 gacggcatac ggatacaagc agatcaaaca cccgaggacc ttgacatgga agacaacgac
      301 ataatcgagg cgcacagaga acagattggt ggcggtccct ctctggactt cgcactcagt
      361 ctgctccgtc gtaatatccg tcaagtgcaa accgatcaag gccatttcac tatgctgggt
      421 gttcgagatc gcctggctgt attgccccgt cacagccagc cgggtaaaac tatctgggtc
      481 gagcacaagt tagtgaagat cgtcgatgcc gttgagctcg tggatgaaca aggggtgaac
      541 ctcgagttga cattagttac gttagataca aacgaaaagt tccgggacat tactcggttc
      601 atacctgaaa ccataagccc tgcttcggac gcgacccttg ttatcaacac agagcatatg
      661 ccgagtatgt ttgtaccagt aggcgatgtg gtacaatacg gctttctcaa tctctccggg
      721 aagcctacac atcggaccat gatgtataac ttcccaacga aggcaggaca atgtggcggg
      781 gttgttacag cagttggaaa agttattggg attcatatag gtggaaacgg ccgccaggga
      841 ttttgtgctg ctttgaagag agggtatttc tgtagtgagc aataatga
//
