LOCUS       sequence-449358-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to generate stable cell lines that express PERK(LD)-EGFP. This 
            plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Xu F, Wang L
  TITLE     Deciphering ER stress-unfolded protein response relationship by 
            visualizing unfolded proteins in the ER.
  JOURNAL   Cell Rep. 2024 Jun 25;43(6):114358. doi: 
            10.1016/j.celrep.2024.114358. Epub 2024 Jun 11.
  PUBMED    38865243
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-06-25"; volume: "43(6):114358. doi"; pages: " 
            10.1016/j.celrep.2024.114358. Epub 2024 Jun 11"
COMMENT     Sequence Label: pLVX-PERK(LD)-EGFP
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2820..2829
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4086..4796
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             4086..4796
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(4125..4146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4386..4405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4733..4754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4847..5346
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4882..4905)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5279..5298
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5367..5966
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5367..5386)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5863..5883
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5980..6568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6033..6053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6451..6462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6775..7408
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7537..7553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7537..7553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7550..7572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7561..7577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7585..7591,7592..7609,7610..7615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7630..7651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7768..7785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7939..8527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8019..8038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8698..9489,9490..9558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9321..9340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9559..9663)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9711..9845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9748..9767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9802..9821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10013,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat cgctagcgct accggactca gatctcgagg
     2821 ccgccatgga gcgcgccacc cggcccgggc cgcgcgcgct gctgctgctt ctgttcctgc
     2881 tgctgggctg cgcggcgggg atctcggcgg tcgcgcccgc ccgcagtttg cttgctcccg
     2941 cgtcggagac agtgtttggc ttaggggcag cggccgcccc gacttcggcc gcgcgggtgc
     3001 ctgcggtggc aacggccgaa gtgaccgtgg aggacgccga ggcattgccg gctgccgctg
     3061 gcgaaccgga gtcacgcgcg acggagcccg atgacgacgt ggaactgcgg cctcgcggca
     3121 ggtccttggt aatcatcagc actttagatg gacgaatcgc tgcactggat gccgagaatg
     3181 atgggaaaaa gcagtgggat ttggacgtgg ggtctggttc cttggtttca tctagcctca
     3241 gcaagccaga ggtgtttggg aacaagatga tcatcccctc cctggatgga gacctcttcc
     3301 agtgggaccg ggaccgagag agcatggagg ccgtcccctt cacggtggag tccctgctcg
     3361 aatcttccta caagtttgga gatgatgttg ttctggttgg agggaaatct ctgactacat
     3421 acggactcag tgcttacagt ggaaagctga ggtatatctg ttctgccttg ggatgtcgcc
     3481 gatgggatag tgatgaaatg gaagaagagg aagacatctt gcttctgcag cgtacgcaga
     3541 agactgtgcg agctgtcggg cctcgaagcg gcagtgagaa gtggaatttc agtgttggcc
     3601 actttgaact tcggtatatt ccagacatgg aaactagagc cggattcatt gaaagcacct
     3661 ttaaaccggg tggaaacaaa gaagactcta aaattatttc agatgtggaa gaacaagaag
     3721 ccaccatgct ggacacagtg ataaaagttt ccgttgctga ttggaaggtc atggcgttta
     3781 gtaggaaggg aggccgcctg gaatgggagt accagttttg tactcccatc gcgtccgcct
     3841 ggctggtgag ggatggcaag gtcatcccca tcagcctgtt tgatgataca agttacacag
     3901 ccagcgaaga agccttggga gacgaagaag acattgtaga ggctgctcgg ggagccacag
     3961 agaacagcgt gtacttaggg atgtacagag gccagctgta cctgcagtcg tccgtcaggg
     4021 tctcagaaaa gttccctaca agcccaaagg ccttggagtc tgtaaatggc gaaggtagtg
     4081 gaagcagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4141 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4201 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4261 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4321 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4381 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4441 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4501 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4561 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4621 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4681 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4741 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaaag
     4801 atgaactgta agggcccggg atcccgcgac tctagataat tctaccgggt aggggaggcg
     4861 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     4921 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     4981 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     5041 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     5101 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     5161 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     5221 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     5281 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     5341 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     5401 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5461 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5521 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     5581 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     5641 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     5701 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     5761 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     5821 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     5881 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     5941 gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct ggattacaaa
     6001 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6061 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6121 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6181 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6241 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6301 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6361 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6421 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6481 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6541 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     6601 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6661 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6721 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     6781 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6841 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     6901 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     6961 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7021 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7081 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     7141 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     7201 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7261 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7321 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7381 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7441 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     7501 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7561 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7621 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7681 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7741 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7801 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7861 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7921 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7981 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8041 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8101 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8161 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8281 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8341 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8401 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8461 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8521 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8581 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8641 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8701 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8761 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8821 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8881 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8941 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9001 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9061 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9121 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9181 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9241 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9301 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9361 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9421 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9481 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9541 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9601 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9661 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
     9721 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9781 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9841 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     9901 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     9961 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gtt
//
