LOCUS       sequence-449420-        4090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Shp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pENTR2b-PTPN11
FEATURES             Location/Qualifiers
     source          1..4090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2319..2418)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2530..2549)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2541..3350
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2608..2627)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3440..4028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3929..3948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccgg taccgaggag atctgccgcc gcgatcgcca tgacatcgcg gagatggttt
      541 cacccaaata tcactggtgt ggaggcagaa aacctactgt tgacaagagg agttgatggc
      601 agttttttgg caaggcctag taaaagtaac cctggagact tcacactttc cgttagaaga
      661 aatggagctg tcacccacat caagattcag aacactggtg attactatga cctgtatgga
      721 ggggagaaat ttgccacttt ggctgagttg gtccagtatt acatggaaca tcacgggcaa
      781 ttaaaagaga agaatggaga tgtcattgag cttaaatatc ctctgaactg tgcagatcct
      841 acctctgaaa ggtggtttca tggacatctc tctgggaaag aagcagagaa attattaact
      901 gaaaaaggaa aacatggtag ttttcttgta cgagagagcc agagccaccc tggagatttt
      961 gttctttctg tgcgcactgg tgatgacaaa ggggagagca atgacggcaa gtctaaagtg
     1021 acccatgtta tgattcgctg tcaggaactg aaatacgacg ttggtggagg agaacggttt
     1081 gattctttga cagatcttgt ggaacattat aagaagaatc ctatggtgga aacattgggt
     1141 acagtactac aactcaagca gccccttaac acgactcgta taaatgctgc tgaaatagaa
     1201 agcagagttc gagaactaag caaattagct gagaccacag ataaagtcaa acaaggcttt
     1261 tgggaagaat ttgagacact acaacaacag gagtgcaaac ttctctacag ccgaaaagag
     1321 ggtcaaaggc aagaaaacaa aaacaaaaat agatataaaa acatcctgcc ctttgatcat
     1381 accagggttg tcctacacga tggtgatccc aatgagcctg tttcagatta catcaatgca
     1441 aatatcatca tgcctgaatt tgaaaccaag tgcaacaatt caaagcccaa aaagagttac
     1501 attgccacac aaggctgcct gcaaaacacg gtgaatgact tttggcggat ggtgttccaa
     1561 gaaaactccc gagtgattgt catgacaacg aaagaagtgg agagaggaaa gagtaaatgt
     1621 gtcaaatact ggcctgatga gtatgctcta aaagaatatg gcgtcatgcg tgttaggaac
     1681 gtcaaagaaa gcgccgctca tgactatacg ctaagagaac ttaaactttc aaaggttgga
     1741 caagggaata cggagagaac ggtctggcaa taccactttc ggacctggcc ggaccacggc
     1801 gtgcccagcg accctggggg cgtgctggac ttcctggagg aggtgcacca taagcaggag
     1861 agcatcatgg atgcagggcc ggtcgtggtg cactgcagtg ctggaattgg ccggacaggg
     1921 acgttcattg tgattgatat tcttattgac atcatcagag agaaaggtgt tgactgcgat
     1981 attgacgttc ccaaaaccat ccagatggtg cggtctcaga ggtcagggat ggtccagaca
     2041 gaagcacagt accgatttat ctatatggcg gtccagcatt atattgaaac actacagcgc
     2101 aggattgaag aagagcagaa aagcaagagg aaagggcacg aatatacaaa tattaagtat
     2161 tctctagcgg accagacgag tggagatcag agccctctcc cgccttgtac tccaacgcca
     2221 ccctgtgcag aaatgagaga agacagtgct agagtctatg aaaacgtggg cctgatgcaa
     2281 cagcagaaaa gcggccgctg ataactcgag atatctagac ccagctttct tgtacaaagt
     2341 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2401 aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat
     2461 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     2521 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     2581 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     2641 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     2701 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     2761 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     2821 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     2881 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     2941 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     3001 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     3061 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     3121 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     3181 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     3241 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     3301 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     3361 ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca gaccccgtag
     3421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3841 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4021 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4081 ctcacatgtt
//
