LOCUS       sequence-449839-        3706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI039
FEATURES             Location/Qualifiers
     source          1..3706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             325..576
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             592..825
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             835..1770
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             883..1776
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     primer_bind     complement(1820..1838)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1906..2010
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2232..3023
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3194..3706,1..76)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3683..3702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatgaaa gcgttaacgg ccaggcaaca agaggtgttt
      361 gatctcatcc gtgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
      421 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
      481 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag
      541 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccgtcgag cgcccccccg
      601 accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtggc gatggcgcat
      661 gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc cccgggtccg
      721 ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga cttcgagttt
      781 gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtggggatcc gtctgctgga
      841 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
      901 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
      961 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
     1021 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
     1081 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
     1141 gagatcctga tgattggtct tgtctggcgc tccatggagc acccagggaa gctactgttt
     1201 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
     1261 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
     1321 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
     1381 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
     1441 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
     1501 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
     1561 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggagatgctg
     1621 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
     1681 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
     1741 acgggggagg cagagggttt ccctgccaca gtctgagctt gctttgagac cacgaagtgg
     1801 ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1861 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1921 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1981 atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg
     2041 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     2101 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2161 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2221 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2281 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2341 cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa
     2401 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2461 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2521 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2581 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2641 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2701 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2761 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2821 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2881 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     2941 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga
     3001 tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg
     3061 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3121 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatag
//
