LOCUS       sequence-449879-        5557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tn libraries Himar variant ERM backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5557)
  TITLE     Cultivarium plasmids
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGLU_9
FEATURES             Location/Qualifiers
     source          1..5557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            161..269
                     /note="incP origin of transfer"
     RBS             1797..1808
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(3628..3644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3628..3644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3641..3663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      3834..4222
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(4355..4379)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4479..5270,5271..5339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5102..5121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5340..5444)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(5543..5557,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5557,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgacctt agcgtgcgaa cagcacgcta ttgttgtatc attactcgca tccattctca
      121 ggctgtctcg tctcgtctcc gagctcttca atgaggagta cggccagcct cgcagagcag
      181 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
      241 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
      301 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
      361 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagtcgttg ggattgtcct
      421 gaccgaagcg ggacaggtcc acgagcaggc aatgcttgtt gggcatggtg aggtggatgt
      481 ctttcacttc cggcacgcag ccgaggactt tttgcgccat atcgtaaagt gtgtgctgaa
      541 cggattcgct ttcgtgttca gcgaaagagg cgagaaggag attccaagct tattcaacat
      601 agttcccttc aagagcgata caacgattat aacgaccttc caattttttg ataccatttt
      661 ggtagtactc cttcggtttt gcctcaaaat aggcctcagt ttcggcgatc acctcttcat
      721 tgcagccaaa ttttttccct gcgagcatcc ttttgaggtc tgagaacaag aaaaagtcgc
      781 tgggggccag atctggagaa tacggcgggt ggggaagcaa ttcgaagccc aattcatgaa
      841 tttttgccat cgttctcaat gacttgtggc acggtgcgtt gtcttggtgg aacaacactt
      901 ttttcttctt catgtggggc cgttttgccg cgatttcgac cttcaaacgc tccaataacg
      961 ccatataata gtcactgttg atggtttttc ccttctcaag ataatcgata aaaattattc
     1021 catgcgcatc ccaaaaaaca gaggccatta ctttgccagc ggacttttga gtctttccac
     1081 gcttcggaga cggttcaccg gtcgctgtcc actcagccga ctgtcgattg gactcaggag
     1141 tgtagtgatg gagccatgtt tcatccattg tcacatatcg acggaaaaac tcgggtgtat
     1201 tacgagttaa cagctgcaaa caccgcttag aatcatcaac acgtcgttgt ttttggtcaa
     1261 atgtgagctc gcgcggcacc catttcgcac agagcttccg catatccaaa tattgatgaa
     1321 tgatatgacc aacacgttcc tttgatatct ttaaggcctc tgctatctcg atcaacttca
     1381 ttttacggtc attcaaaatc attttgtgga tttttttgat gttttcgtcg gtaaccacct
     1441 ctttcgggcg tccactgcgt tcaccgtcct ccgtgctcat ttcaccacgc ttgaattttg
     1501 cataccaatc aattattgtt gatttccctg gggcagagtc cggaaactca ttatcaagcc
     1561 aagtttttgc ttccactgta ttttttccct tcagaaaaca gtattttatc aaaacacgaa
     1621 attccttttt ttccattgag acccagtcga aagtgaaacg tgatttcatg cgtcattttg
     1681 aacattttgt aaatcttatt taataatgtg tgcggcaatt cacatttaat ttatgaatgt
     1741 tttcttaaca tcgcggcaac tcaagaaacg gcaggttcgg atcttagcta ctagagaaag
     1801 aggagaaata ctagatggtg agtaagggtg aagccgtgat taaagagttc atgcgcttca
     1861 aagtgcatat ggagggctca atgaacggcc atgaatttga aattgaaggt gaaggagaag
     1921 gccgtccgta tgagggcacc cagaccgcca aactgaaagt cacaaagggt ggcccgctgc
     1981 cgttttcgtg ggatatttta tcccctcaat tcatgtacgg cagccgggcg tttattaaac
     2041 acccggcgga tatcccggat tactataagc aatcttttcc agaaggcttt aaatgggagc
     2101 gcgtgatgaa ttttgaagac ggaggtgctg ttaccgtgac ccaggacacg agcttagaag
     2161 acggtacctt gatctataag gtaaagctgc gtggtaccaa tttcccgccg gatgggccag
     2221 ttatgcagaa aaagactatg ggctgggaag catcgacgga gcgtctttac cccgaagacg
     2281 gcgtcctgaa aggcgatatt aaaatggccc tgcgactgaa agacggcggt cgctaccttg
     2341 cagatttcaa aacaacgtat aaagcgaaaa aagcgaaaaa acctgtgcag atgcccgggg
     2401 cgtacaacgt tgatcgcaaa ctagatatca cttcacacaa cgaagattac acggtagtcg
     2461 aacagtatga acgtagcgag gggcgccata gcaccggagg catggatgaa ctctataaat
     2521 aattcagcca aaaaacttaa gaccgccggt cttgtccact accttgcagt aatgcggtgg
     2581 acaggatcgg cggttttctt ttctcttctc aagactgggt ctcaaatgaa cgagaaaaat
     2641 ataaaacaca gtcaaaactt tattacttct aagcataata tcgacaagat tatgacaaac
     2701 ataagattaa atgaacatga taatatcttt gaaatcggct caggaaaagg ccattttacc
     2761 cttgaattag taaagaggtg taatttcgta actgccattg aaatagacca taaattatgc
     2821 aaaactacag aaaataaact tgttgatcac gataatttcc aagttttaaa caaggatata
     2881 ttgcagttta aatttcctaa aaaccaatcc tataaaatat atggtaatat accttataac
     2941 ataagtacgg atataatacg caaaattgtt tttgatagta tagctaatga gatttattta
     3001 atcgtggaat acgggtttgc taaaagatta ttaaatacaa aacgctcatt ggcattactt
     3061 ttaatggcag aagttgatat ttctatatta agtatggttc caagagaata ttttcatcct
     3121 aaacctaaag tgaatagctc acttatcaga ttaagtagaa aaaaatcaag aatatcacac
     3181 aaagataaac aaaagtataa ttatttcgtt atgaaatggg ttaacaaaga atacaagaaa
     3241 atatttacaa aaaatcaatt taacaattcc ttaaaacatg caggaattga cgatttaaac
     3301 aatattagct ttgaacaatt cttatctctt ttcaatagct ataaattatt taataagtaa
     3361 gcttgttaag ggatgcataa actgcatccc ttaacttgtt tttcgtgtgc ctattttttg
     3421 tgaatcgatt atgtcttttg cgcagtcggc tagaccgggg acttatcatc caacctgtta
     3481 cgctagcatg aagagcaccc caggatacat agattaccac aactccgagc ccttccacct
     3541 agcgtgctaa ccacgcacgc tattgttgta taggctaggt ggaggctcag tgatgataag
     3601 tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc
     3661 gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt
     3721 tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag
     3781 gccaggaacc gtaaaaaggc cgcgttgctg gcgttacagc tgggcgcgcc ccatgtcagc
     3841 cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc
     3901 gttacatccc tggcttgttg tccacaaccg ttaaacctta aaggctttaa aagccttata
     3961 tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata
     4021 ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta
     4081 gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt
     4141 acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg
     4201 ttgaactgct gatcttcaga tcggcgcgcc ggccggccga agatcctttg atcttttcta
     4261 cggggtctga cgctctattc aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag
     4321 ggggcttcaa atcgtcctcg tgataccaat tcggagcctg cttttttgta caaacttgtt
     4381 gataatggca attcaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4621 gagccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5461 gtgccagata cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat
     5521 ccaccacaag cgccagggtt ttcccagtca cgacgtt
//
